LOCUS       BBa_I715011             2840 bp    DNA     linear       21-Dec-2024
DEFINITION  Lambda Model Promoter:RBS:Kan:Tet:RBS:RFP
ACCESSION   BBa_I715011
VERSION     BBa_I715011.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..891
                     /label=KanR Gene
     CDS             76..891
                     /label=BBa_J31003
     misc_feature    58..891
                     /label=BBa_S03709
     misc_feature    904..907
                     /label=conserved
     RBS             900..911
                     /label=BBa_B0034
     CDS             918..2108
                     /label=tetA(C) forward
     primer_bind     918..941
                     /label=Fwd primer J31007
     primer_bind     2089..2108
                     /label=Rev primer J31007
     misc_feature    2106..2108
                     /label=stop
     CDS             918..2108
                     /label=BBa_J31007
     misc_feature    900..2108
                     /label=BBa_S03710
     misc_feature    2121..2124
                     /label=conserved
     RBS             2117..2128
                     /label=BBa_B0034
     CDS             2135..2809
                     /label=mrfp1
     misc_feature    2816..2840
                     /label=Help:Barcodes
     CDS             2135..2815
                     /label=BBa_E1010
     misc_feature    2117..2840
                     /label=BBa_I13502
     misc_feature    900..2840
                     /label=BBa_S03720
     misc_feature    58..2840
                     /label=BBa_S03723
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgag ccatattcaa cgggaaacgt cttgctccag gccgcgatta
      121 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
      181 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
      241 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
      301 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
      361 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat
      421 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
      481 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
      541 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
      601 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
      661 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
      721 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
      781 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
      841 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atactagaga
      901 aagaggagaa atactagatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca
      961 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata
     1021 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga
     1081 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag
     1141 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg
     1201 tcctgtggat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     1261 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     1321 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     1381 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     1441 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg atgcccttga
     1501 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     1561 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca
     1621 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat
     1681 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg ccccgccacc aaacgtttcg
     1741 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg
     1801 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca
     1861 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac
     1921 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg
     1981 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg
     2041 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct
     2101 cgacctaata ctagagaaag aggagaaata ctagatggct tcctccgaag acgttatcaa
     2161 agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat
     2221 cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac
     2281 caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc
     2341 caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga
     2401 aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca
     2461 ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt
     2521 cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg
     2581 tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga
     2641 cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct
     2701 gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac
     2761 catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg
     2821 atagtgctag tgtagatcgc
//