LOCUS       BBa_I715012             1301 bp    DNA     linear       25-Nov-2024
DEFINITION  pLac-RBS-CreA-hixC-CreB
ACCESSION   BBa_I715012
VERSION     BBa_I715012.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    227..796
                     /label=CreA
     CDS             227..796
                     /label=BBa_I715000
     misc_feature    805..830
                     /label=BBa_J44000
     misc_feature    840..1298
                     /label=CreB
     misc_feature    1299..1301
                     /label=stop
     misc_feature    839..839
                     /label=Inserted Base
     CDS             839..1301
                     /label=BBa_I715001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt ccaatttact
      241 gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa
      301 gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct
      361 tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc
      421 cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt
      481 aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc
      541 acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt
      601 tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt
      661 tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg
      721 gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat
      781 ctcacgtact gacggttact agagttatca aaaaccatgg tttttgataa tactagagtg
      841 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga
      901 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag
      961 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat
     1021 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat
     1081 tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg
     1141 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc
     1201 aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg
     1261 aaacaggggc aatggtgcgc ctgctggaag atggcgatta g
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