LOCUS       BBa_I715013             2324 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB3K3)
ACCESSION   BBa_I715013
VERSION     BBa_I715013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..242
                     /label=BBa_J61046
     CDS             251..928
                     /label=mRFP reverse
     primer_bind     909..928
                     /label=Spe mRFP F
     primer_bind     251..270
                     /label=Xba mRFP R
     misc_feature    251..928
                     /label=BBa_J31008
     stem_loop       948..991
                     /label=stem_loop
     misc_feature    937..1016
                     /label=BBa_B0010
     terminator      937..1016
                     /label=BBa_B0010
     stem_loop       1032..1051
                     /label=T7 TE
     misc_feature    1058..1058
                     /label=stop
     polyA_site      1052..1065
                     /label=polya
     misc_feature    1025..1065
                     /label=BBa_B0012
     terminator      1025..1065
                     /label=BBa_B0012
     misc_feature    1074..1107
                     /label=BBa_J61046
     misc_feature    1120..1123
                     /label=conserved
     RBS             1116..1127
                     /label=BBa_B0034
     CDS             1134..2324
                     /label=tetA(C) forward
     primer_bind     1134..1157
                     /label=Fwd primer J31007
     primer_bind     2305..2324
                     /label=Rev primer J31007
     misc_feature    2322..2324
                     /label=stop
     CDS             1134..2324
                     /label=BBa_J31007
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat aacttcgtat aatgtatgct atacgaagtt
      241 attactagag ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga
      301 tggtgtagtc ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg
      361 gcagctgaac cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac
      421 cgtctttcag tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca
      481 tacgttcggt ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg
      541 ggaagttggt accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt
      601 cctgggtaac ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac
      661 cttccgggaa ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt
      721 tggaaccgta ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt
      781 tggtaacttt cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt
      841 cgatttcgaa ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact
      901 ctttgataac gtcttcggag gaagccatta ctagagccag gcatcaaata aaacgaaagg
      961 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1021 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagataactt
     1081 cgtataatgt atgctatacg aagttattac tagagaaaga ggagaaatac tagatgaaat
     1141 ctaacaatgc gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct
     1201 tggttatgcc ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca
     1261 gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc
     1321 tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag
     1381 ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac
     1441 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
     1501 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
     1561 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
     1621 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
     1681 agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     1741 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     1801 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct
     1861 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc
     1921 aagccttcgt cactggcccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg
     1981 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg
     2041 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
     2101 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
     2161 ttaccagcct aacttcgatc attggaccgc tgatcgtcac ggcgatttat gccgcctcgg
     2221 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
     2281 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctaa
//