BBa_I715013
type: ComponentDefinition
persistent identity: BBa I715013
display id: BBa_I715013
version: 1
was generated by: iGEM to SBOL conversion
description: pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB3K3)
created: 2007-07-04T11:00:00Z
modified: 2015-08-31T04:07:49Z
mutable provenance: See Subparts.
owned by: james
owned by: myers
mutable description: This part is designed to report the presence of functional Cre Recombinase. We plan to put it into plasmid pSB3K3 and cotransform it with BBa_I715012 to test the functionality of our Cre Recombinase with a hixC site inserted between amino acids 190 and 191.
discontinued: false
dominant: true
experience: experience/Fails
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is in plasmid pSB1A2. We plan to put it into pSB3K3 to perform a cotransformation with BBa_I715012.
star: false
creator: Will DeLoache
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_R0010
component: BBa_B0012
component: BBa_B0010
component: BBa_J31008
component: BBa_J61046
component: BBa_J31007
component: BBa_B0034
sequence annotation: BBa_R0010
sequence annotation: BBa_J61046
sequence annotation: BBa_J31008
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_J31007
sequence: https://synbiohub.org/public/igem/BBa_I715013_sequence/1

BBa_R0010
type: Component
persistent identity: component1936791
display id: component1936791
version: 1
top level: BBa_I715013
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1936805
display id: component1936805
version: 1
top level: BBa_I715013
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1936803
display id: component1936803
version: 1
top level: BBa_I715013
definition: BBa_B0010
access: public

BBa_J31008
type: Component
persistent identity: component1936802
display id: component1936802
version: 1
top level: BBa_I715013
definition: mRFPr
access: public

BBa_J61046
type: Component
persistent identity: component1936798
display id: component1936798
version: 1
top level: BBa_I715013
definition: lox
access: public

BBa_J31007
type: Component
persistent identity: component1936816
display id: component1936816
version: 1
top level: BBa_I715013
definition: tetA(C)f
access: public

BBa_B0034
type: Component
persistent identity: component1936811
display id: component1936811
version: 1
top level: BBa_I715013
definition: BBa_B0034
access: public

BBa_J61046
type: Component
persistent identity: component1936809
display id: component1936809
version: 1
top level: BBa_I715013
definition: lox
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1936791
display id: annotation1936791
version: 1
top level: BBa_I715013
location: https://synbiohub.org/public/igem/BBa_I715013/annotation1936791/range1936791/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I715013/annotation1936791/range1936791/1
type: Range
persistent identity: range1936791
display id: range1936791
version: 1
top level: BBa_I715013
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_J61046
type: SequenceAnnotation
persistent identity: annotation1936798
display id: annotation1936798
version: 1
top level: BBa_I715013
location: https://synbiohub.org/public/igem/BBa_I715013/annotation1936798/range1936798/1
component: BBa_J61046

https://synbiohub.org/public/igem/BBa_I715013/annotation1936798/range1936798/1
type: Range
persistent identity: range1936798
display id: range1936798
version: 1
top level: BBa_I715013
direction: direction/reverse
start: 209
end: 242
orientation: inline

BBa_J31008
type: SequenceAnnotation
persistent identity: annotation1936802
display id: annotation1936802
version: 1
top level: BBa_I715013
location: https://synbiohub.org/public/igem/BBa_I715013/annotation1936802/range1936802/1
component: BBa_J31008

https://synbiohub.org/public/igem/BBa_I715013/annotation1936802/range1936802/1
type: Range
persistent identity: range1936802
display id: range1936802
version: 1
top level: BBa_I715013
direction: direction/reverse
start: 251
end: 928
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1936803
display id: annotation1936803
version: 1
top level: BBa_I715013
location: https://synbiohub.org/public/igem/BBa_I715013/annotation1936803/range1936803/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715013/annotation1936803/range1936803/1
type: Range
persistent identity: range1936803
display id: range1936803
version: 1
top level: BBa_I715013
direction: direction/forward
start: 937
end: 1016
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1936805
display id: annotation1936805
version: 1
top level: BBa_I715013
location: https://synbiohub.org/public/igem/BBa_I715013/annotation1936805/range1936805/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715013/annotation1936805/range1936805/1
type: Range
persistent identity: range1936805
display id: range1936805
version: 1
top level: BBa_I715013
direction: direction/forward
start: 1025
end: 1065
orientation: inline

BBa_J61046
type: SequenceAnnotation
persistent identity: annotation1936809
display id: annotation1936809
version: 1
top level: BBa_I715013
location: https://synbiohub.org/public/igem/BBa_I715013/annotation1936809/range1936809/1
component: BBa_J61046

https://synbiohub.org/public/igem/BBa_I715013/annotation1936809/range1936809/1
type: Range
persistent identity: range1936809
display id: range1936809
version: 1
top level: BBa_I715013
direction: direction/reverse
start: 1074
end: 1107
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1936811
display id: annotation1936811
version: 1
top level: BBa_I715013
location: https://synbiohub.org/public/igem/BBa_I715013/annotation1936811/range1936811/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715013/annotation1936811/range1936811/1
type: Range
persistent identity: range1936811
display id: range1936811
version: 1
top level: BBa_I715013
direction: direction/reverse
start: 1116
end: 1127
orientation: inline

BBa_J31007
type: SequenceAnnotation
persistent identity: annotation1936816
display id: annotation1936816
version: 1
top level: BBa_I715013
location: https://synbiohub.org/public/igem/BBa_I715013/annotation1936816/range1936816/1
component: BBa_J31007

https://synbiohub.org/public/igem/BBa_I715013/annotation1936816/range1936816/1
type: Range
persistent identity: range1936816
display id: range1936816
version: 1
top level: BBa_I715013
direction: direction/reverse
start: 1134
end: 2324
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lox
type: ComponentDefinition
persistent identity: BBa J61046
display id: BBa_J61046
version: 1
was generated by: iGEM to SBOL conversion
description: [Lox] site for recombination
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J61046_sequence/1

tetA(C)f
type: ComponentDefinition
persistent identity: BBa J31007
display id: BBa_J31007
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (forward), [cf. BBa_J31006]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This part is the coding region of the TetR gene. It requires a promoter and RBS for the cell to be able to express tetracyline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plamsid.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: tetA(C) forward
sequence annotation: Fwd primer J31007
sequence annotation: Rev primer J31007
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_J31007_sequence/1

tetA(C) forward
type: SequenceAnnotation
persistent identity: annotation1885000
display id: annotation1885000
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J31007/annotation1885000/range1885000/1
type: Range
persistent identity: range1885000
display id: range1885000
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 1191
orientation: inline

Fwd primer J31007
type: SequenceAnnotation
persistent identity: annotation1910571
display id: annotation1910571
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910571/range1910571/1
type: Range
persistent identity: range1910571
display id: range1910571
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1
end: 24
orientation: inline

Rev primer J31007
type: SequenceAnnotation
persistent identity: annotation1910572
display id: annotation1910572
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_J31007/annotation1910572/range1910572/1
type: Range
persistent identity: range1910572
display id: range1910572
version: 1
top level: tetA(C)f
direction: direction/reverse
start: 1172
end: 1191
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1910573
display id: annotation1910573
version: 1
top level: tetA(C)f
location: https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J31007/annotation1910573/range1910573/1
type: Range
persistent identity: range1910573
display id: range1910573
version: 1
top level: tetA(C)f
direction: direction/forward
start: 1189
end: 1191
orientation: inline

mRFPr
type: ComponentDefinition
persistent identity: BBa J31008
display id: BBa_J31008
version: 1
was generated by: iGEM to SBOL conversion
description: RFP reverse
created: 2007-02-15T12:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: RFP reverse was PCR amplified from mRFP (BBaE1010).
owned by: james
owned by: myers
mutable description: The mRFP coding sequence in the reverse orientation. This part requires a reverse RBS and reverse promoter to be expressed
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RFP reverse was PCR amplified from mRFP (BBaE1010) using the following primers: 5'-atgcactagtatggcttcctccgaagacgt "Spe mRFP F" 5'-gcattctagattaagcaccggtggagtgac "Xba mRFP R"
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mRFP reverse
sequence annotation: Xba mRFP R
sequence annotation: Spe mRFP F
sequence: https://synbiohub.org/public/igem/BBa_J31008_sequence/1

mRFP reverse
type: SequenceAnnotation
persistent identity: annotation1911761
display id: annotation1911761
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
type: Range
persistent identity: range1911761
display id: range1911761
version: 1
top level: mRFPr
direction: direction/reverse
start: 1
end: 678
orientation: inline

Xba mRFP R
type: SequenceAnnotation
persistent identity: annotation1911765
display id: annotation1911765
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
type: Range
persistent identity: range1911765
display id: range1911765
version: 1
top level: mRFPr
direction: direction/forward
start: 1
end: 20
orientation: inline

Spe mRFP F
type: SequenceAnnotation
persistent identity: annotation1911764
display id: annotation1911764
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
type: Range
persistent identity: range1911764
display id: range1911764
version: 1
top level: mRFPr
direction: direction/reverse
start: 659
end: 678
orientation: inline

https://synbiohub.org/public/igem/BBa_I715013_sequence/1
type: Sequence
persistent identity: BBa I715013 sequence
display id: BBa_I715013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagataacttcgtataatgtatgctatacgaagttattactagagttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagataacttcgtataatgtatgctatacgaagttattactagagaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61046_sequence/1
type: Sequence
persistent identity: BBa J61046 sequence
display id: BBa_J61046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ataacttcgtataatgtatgctatacgaagttat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31007_sequence/1
type: Sequence
persistent identity: BBa J31007 sequence
display id: BBa_J31007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31008_sequence/1
type: Sequence
persistent identity: BBa J31008 sequence
display id: BBa_J31008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z