LOCUS       BBa_I715014             1263 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac-RBS-Cre
ACCESSION   BBa_I715014
VERSION     BBa_I715014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=R0010 reverse
     CDS             113..200
                     /label=end of LacI coding region (inactive)
     misc_feature    74..112
                     /label=CAP binding site
     promoter        58..64
                     /label=-35
     promoter        35..40
                     /label=-10
     misc_feature    1..34
                     /label=LacI binding site
     promoter        1..200
                     /label=BBa_J31013
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1263
                     /label=BBa_J61047
ORIGIN
        1 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
       61 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      121 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      181 gagaggcggt ttgcgtattg tactagagaa agaggagaaa tactagatgt ccaatttact
      241 gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa
      301 gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct
      361 tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc
      421 cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt
      481 aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc
      541 acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt
      601 tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt
      661 tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg
      721 gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat
      781 ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag
      841 caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat
      901 ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa
      961 tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt
     1021 tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc
     1081 ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc
     1141 aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat
     1201 ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaaga
     1261 att
//