LOCUS       BBa_I715015             1045 bp    DNA     linear       25-Nov-2024
DEFINITION  pLacB:RBS.1:Kan
ACCESSION   BBa_I715015
VERSION     BBa_I715015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=R0010 reverse
     CDS             113..200
                     /label=end of LacI coding region (inactive)
     misc_feature    74..112
                     /label=CAP binding site
     promoter        58..64
                     /label=-35
     promoter        35..40
                     /label=-10
     misc_feature    1..34
                     /label=LacI binding site
     promoter        1..200
                     /label=BBa_J31013
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..1045
                     /label=KanR Gene
     CDS             230..1045
                     /label=BBa_J31003
ORIGIN
        1 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
       61 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      121 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      181 gagaggcggt ttgcgtattg tactagagat taaagaggag aaatactaga tgagccatat
      241 tcaacgggaa acgtcttgct ccaggccgcg attaaattcc aacatggatg ctgatttata
      301 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta
      361 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
      421 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
      481 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa
      541 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
      601 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
      661 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
      721 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
      781 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
      841 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
      901 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
      961 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1021 tttgatgctc gatgagtttt tctaa
//