LOCUS       BBa_I715016              889 bp    DNA     linear       25-Nov-2024
DEFINITION  pLacB:RBS.1:CAT
ACCESSION   BBa_I715016
VERSION     BBa_I715016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..200
                     /label=R0010 reverse
     CDS             113..200
                     /label=end of LacI coding region (inactive)
     misc_feature    74..112
                     /label=CAP binding site
     promoter        58..64
                     /label=-35
     promoter        35..40
                     /label=-10
     misc_feature    1..34
                     /label=LacI binding site
     promoter        1..200
                     /label=BBa_J31013
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     misc_feature    230..889
                     /label=CmR gene
     CDS             230..889
                     /label=BBa_J31005
ORIGIN
        1 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
       61 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      121 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      181 gagaggcggt ttgcgtattg tactagagat taaagaggag aaatactaga tggagaaaaa
      241 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      301 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      361 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      421 ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg agctggtgat
      481 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc
      541 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt
      601 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt
      661 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga
      721 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct
      781 gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat
      841 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaa
//