BBa_I715016
1
BBa_I715016
pLacB:RBS.1:CAT
2007-06-19T11:00:00Z
2015-08-31T04:07:49Z
Ligation of Promoter region, RBS and Chlor Resistance from E.Coli genome
Tests backwards promotion in an uninsulated plasmid on Chlor plates
false
false
_120_
0
1761
9
In stock
false
none
false
Michael Waters
component1934989
1
BBa_J31005
component1934986
1
BBa_B0030
component1934984
1
BBa_J31013
annotation1934989
1
BBa_J31005
range1934989
1
230
889
annotation1934984
1
BBa_J31013
range1934984
1
1
200
annotation1934986
1
BBa_B0030
range1934986
1
209
223
BBa_J31013
1
BBa_J31013
pLac Backwards [cf. BBa_R0010]
2007-04-11T11:00:00Z
2015-08-31T04:08:45Z
BBa_R0010
Promoter from the lack operon cloned in the reverse orientation.
false
false
_61_
0
1144
61
Not in stock
false
Promoter from the lack operon cloned in the reverse orientation.
false
Karmella Haynes
annotation1928670
1
CAP binding site
range1928670
1
74
112
annotation1928671
1
-35
range1928671
1
58
64
annotation1928672
1
-10
range1928672
1
35
40
annotation1928669
1
end of LacI coding region (inactive)
range1928669
1
113
200
annotation1928668
1
R0010 reverse
range1928668
1
1
200
annotation1928673
1
LacI binding site
range1928673
1
1
34
BBa_J31005
1
CmR
chloramphenicol acetyltransferase (forwards, CmF) [cf. BBa_J31004]
2006-07-11T11:00:00Z
2015-08-31T04:08:45Z
pSB1AC3
When a promoter and an RBS are in front of the gene, the cell will express Chloramphenicol resistance. Because it contains full biobrick ends, this part can be used to easily add chloramphenicol resistance to any part without changing plasmid vectors.
false
true
_61_
0
918
61
In stock
true
This part is cloned into pSB1A2.
true
Erin Zwack, Sabriya Rosemond
annotation1884999
1
CmR gene
range1884999
1
1
660
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_I715016_sequence
1
tgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgtactagagattaaagaggagaaatactagatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaa
BBa_J31005_sequence
1
atggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaa
BBa_J31013_sequence
1
tgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z