LOCUS       BBa_I715028             2991 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac-RBS-Kan-RBS-RFP-RBS-Tet
ACCESSION   BBa_I715028
VERSION     BBa_I715028.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1042
                     /label=KanR Gene
     CDS             227..1042
                     /label=BBa_J31003
     misc_feature    209..1042
                     /label=BBa_S03709
     misc_feature    1055..1058
                     /label=conserved
     RBS             1051..1062
                     /label=BBa_B0034
     CDS             1069..1743
                     /label=mrfp1
     misc_feature    1750..1774
                     /label=Help:Barcodes
     CDS             1069..1749
                     /label=BBa_E1010
     misc_feature    1051..1774
                     /label=BBa_I13502
     misc_feature    209..1774
                     /label=BBa_S03711
     misc_feature    1787..1790
                     /label=conserved
     RBS             1783..1794
                     /label=BBa_B0034
     CDS             1801..2991
                     /label=tetA(C) forward
     primer_bind     1801..1824
                     /label=Fwd primer J31007
     primer_bind     2972..2991
                     /label=Rev primer J31007
     misc_feature    2989..2991
                     /label=stop
     CDS             1801..2991
                     /label=BBa_J31007
     misc_feature    1783..2991
                     /label=BBa_S03710
     misc_feature    209..2991
                     /label=BBa_S03721
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gccatattca
      241 acgggaaacg tcttgctcca ggccgcgatt aaattccaac atggatgctg atttatatgg
      301 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
      361 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
      421 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
      481 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
      541 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
      601 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
      661 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
      721 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
      781 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
      841 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
      901 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
      961 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     1021 gatgctcgat gagtttttct aatactagag aaagaggaga aatactagat ggcttcctcc
     1081 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
     1141 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
     1201 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
     1261 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
     1321 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
     1381 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
     1441 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
     1501 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt
     1561 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
     1621 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
     1681 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
     1741 gcttaataac gctgatagtg ctagtgtaga tcgctactag agaaagagga gaaatactag
     1801 atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc
     1861 ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc
     1921 atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca
     1981 cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta
     2041 cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac
     2101 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     2161 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     2221 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     2281 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     2341 atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
     2401 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     2461 atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     2521 tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     2581 ctcgctcaag ccttcgtcac tggccccgcc accaaacgtt tcggcgagaa gcaggccatt
     2641 atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     2701 tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     2761 caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     2821 gcggctctta ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc
     2881 gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     2941 tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgaccta a
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