LOCUS BBa_I715029 2899 bp DNA linear 26-Nov-2024 DEFINITION OmpC-RBS-Kan-RBS-RFP-RBS-Tet ACCESSION BBa_I715029 VERSION BBa_I715029.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 CDS 135..950 /label=KanR Gene CDS 135..950 /label=BBa_J31003 misc_feature 117..950 /label=BBa_S03709 misc_feature 963..966 /label=conserved RBS 959..970 /label=BBa_B0034 CDS 977..1651 /label=mrfp1 misc_feature 1658..1682 /label=Help:Barcodes CDS 977..1657 /label=BBa_E1010 misc_feature 959..1682 /label=BBa_I13502 misc_feature 117..1682 /label=BBa_S03711 misc_feature 1695..1698 /label=conserved RBS 1691..1702 /label=BBa_B0034 CDS 1709..2899 /label=tetA(C) forward primer_bind 1709..1732 /label=Fwd primer J31007 primer_bind 2880..2899 /label=Rev primer J31007 misc_feature 2897..2899 /label=stop CDS 1709..2899 /label=BBa_J31007 misc_feature 1691..2899 /label=BBa_S03710 misc_feature 117..2899 /label=BBa_S03721 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag 121 aggagaaata ctagatgagc catattcaac gggaaacgtc ttgctccagg ccgcgattaa 181 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 241 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac 301 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 361 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 421 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt 481 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 541 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 601 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 661 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc 721 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 781 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 841 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 901 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tactagagaa 961 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa 1021 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg 1081 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc 1141 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca 1201 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg 1261 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga 1321 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt 1381 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg 1441 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc 1501 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac 1561 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga 1621 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc 1681 gctactagag aaagaggaga aatactagat gaaatctaac aatgcgctca tcgtcatcct 1741 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct 1801 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct 1861 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg 1921 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc 1981 gaccacaccc gtcctgtgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc 2041 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg 2101 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg 2161 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg 2221 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc 2281 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 2341 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 2401 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc 2461 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gccccgccac 2521 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta 2581 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc 2641 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga 2701 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg 2761 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg 2821 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag 2881 ccgggccacc tcgacctaa //