LOCUS       BBa_I715030             2840 bp    DNA     linear       26-Nov-2024
DEFINITION  lambda lambda mod-RBS-Kan-RBS-RFP-RBS-Tet
ACCESSION   BBa_I715030
VERSION     BBa_I715030.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..891
                     /label=KanR Gene
     CDS             76..891
                     /label=BBa_J31003
     misc_feature    58..891
                     /label=BBa_S03709
     misc_feature    904..907
                     /label=conserved
     RBS             900..911
                     /label=BBa_B0034
     CDS             918..1592
                     /label=mrfp1
     misc_feature    1599..1623
                     /label=Help:Barcodes
     CDS             918..1598
                     /label=BBa_E1010
     misc_feature    900..1623
                     /label=BBa_I13502
     misc_feature    58..1623
                     /label=BBa_S03711
     misc_feature    1636..1639
                     /label=conserved
     RBS             1632..1643
                     /label=BBa_B0034
     CDS             1650..2840
                     /label=tetA(C) forward
     primer_bind     1650..1673
                     /label=Fwd primer J31007
     primer_bind     2821..2840
                     /label=Rev primer J31007
     misc_feature    2838..2840
                     /label=stop
     CDS             1650..2840
                     /label=BBa_J31007
     misc_feature    1632..2840
                     /label=BBa_S03710
     misc_feature    58..2840
                     /label=BBa_S03721
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatgag ccatattcaa cgggaaacgt cttgctccag gccgcgatta
      121 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
      181 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
      241 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
      301 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
      361 ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga atatcctgat
      421 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
      481 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
      541 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
      601 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
      661 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
      721 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
      781 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
      841 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atactagaga
      901 aagaggagaa atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca
      961 aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag
     1021 gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc
     1081 cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac
     1141 acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac
     1201 gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag
     1261 acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg
     1321 ttatgcagaa aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg
     1381 gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg
     1441 ctgaagttaa aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa
     1501 ccgacatcaa actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg
     1561 aacgtgctga aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat
     1621 cgctactaga gaaagaggag aaatactaga tgaaatctaa caatgcgctc atcgtcatcc
     1681 tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc
     1741 tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc
     1801 tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg
     1861 gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg
     1921 cgaccacacc cgtcctgtgg atcctctacg ccggacgcat cgtggccggc atcaccggcg
     1981 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     2041 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     2101 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     2161 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac
     2221 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     2281 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     2341 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
     2401 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggccccgcca
     2461 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
     2521 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
     2581 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
     2641 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg
     2701 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
     2761 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
     2821 gccgggccac ctcgacctaa
//