##gff-version 3
##sequence-region BBa_I715030 1 2840
BBa_I715030	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_I715030	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_I715030	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_I715030	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_I715030	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_I715030	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_I715030	.	engineered_region	58	2840	.	+	0	ID=BBa_S03721;Name=BBa_S03721
BBa_I715030	.	engineered_region	58	1623	.	+	0	ID=BBa_S03711;Name=BBa_S03711;Parent=BBa_S03721
BBa_I715030	.	engineered_region	900	1623	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_S03711
BBa_I715030	.	ribosome_entry_site	900	911	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_I715030	.	conserved_region	904	907	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715030	.	CDS	918	1598	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_I715030	.	engineered_tag	1599	1623	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I715030	.	CDS	918	1592	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I715030	.	engineered_region	58	891	.	+	0	ID=BBa_S03709;Name=BBa_S03709;Parent=BBa_S03711
BBa_I715030	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03709
BBa_I715030	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715030	.	CDS	76	891	.	+	0	ID=BBa_J31003;Name=BBa_J31003;Parent=BBa_S03709
BBa_I715030	.	CDS	76	891	.	+	0	ID=annotation1884996;Name=KanR Gene;Parent=BBa_J31003
BBa_I715030	.	engineered_region	1632	2840	.	+	0	ID=BBa_S03710;Name=BBa_S03710;Parent=BBa_S03721
BBa_I715030	.	ribosome_entry_site	1632	1643	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03710
BBa_I715030	.	conserved_region	1636	1639	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715030	.	CDS	1650	2840	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_S03710
BBa_I715030	.	primer_binding_site	1650	1673	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_I715030	.	primer_binding_site	2821	2840	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_I715030	.	CDS	1650	2840	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_I715030	.	stop_codon	2838	2840	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
>BBa_I715030
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatga
gccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggtataaatg
ggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctg
aaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctc
ttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaacagcatt
ccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcg
attcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtt
tggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagctttt
gccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaata
ggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagt
tttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcattt
gatgctcgatgagtttttctaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagt
tcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtcc
gtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccg
cagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaag
gtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacgg
tgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggt
tgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaag
acggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaac
cgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcac
tccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgaaatcta
acaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccggg
cctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaa
tttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggag
ccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcac
cggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctc
atgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcac
cattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggaga
gcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgca
cttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgct
ttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcac
tggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttg
ctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgt
tgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcct
aacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggatt
gtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaa