LOCUS       BBa_I715031             2846 bp    DNA     linear       26-Nov-2024
DEFINITION  Hyb pLac-RBS-Kan-RBS-RFP-RBS-Tet
ACCESSION   BBa_I715031
VERSION     BBa_I715031.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..897
                     /label=KanR Gene
     CDS             82..897
                     /label=BBa_J31003
     misc_feature    64..897
                     /label=BBa_S03709
     misc_feature    910..913
                     /label=conserved
     RBS             906..917
                     /label=BBa_B0034
     CDS             924..1598
                     /label=mrfp1
     misc_feature    1605..1629
                     /label=Help:Barcodes
     CDS             924..1604
                     /label=BBa_E1010
     misc_feature    906..1629
                     /label=BBa_I13502
     misc_feature    64..1629
                     /label=BBa_S03711
     misc_feature    1642..1645
                     /label=conserved
     RBS             1638..1649
                     /label=BBa_B0034
     CDS             1656..2846
                     /label=tetA(C) forward
     primer_bind     1656..1679
                     /label=Fwd primer J31007
     primer_bind     2827..2846
                     /label=Rev primer J31007
     misc_feature    2844..2846
                     /label=stop
     CDS             1656..2846
                     /label=BBa_J31007
     misc_feature    1638..2846
                     /label=BBa_S03710
     misc_feature    64..2846
                     /label=BBa_S03721
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgagccat attcaacggg aaacgtcttg ctccaggccg
      121 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      181 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
      241 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
      301 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
      361 gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt agaagaatat
      421 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
      481 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
      541 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
      601 cctgttgaac aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc
      661 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
      721 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
      781 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
      841 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatac
      901 tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc
      961 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
     1021 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc
     1081 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg
     1141 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat
     1201 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc
     1261 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
     1321 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
     1381 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact
     1441 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
     1501 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
     1561 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt
     1621 gtagatcgct actagagaaa gaggagaaat actagatgaa atctaacaat gcgctcatcg
     1681 tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc
     1741 cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc
     1801 tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc
     1861 gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga
     1921 tcatggcgac cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca
     1981 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     2041 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     2101 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
     2161 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     2221 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     2281 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     2341 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
     2401 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggcc
     2461 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
     2521 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
     2581 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
     2641 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
     2701 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
     2761 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
     2821 catggagccg ggccacctcg acctaa
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