##gff-version 3
##sequence-region BBa_I715031 1 2846
BBa_I715031	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I715031	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I715031	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I715031	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I715031	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I715031	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I715031	.	engineered_region	64	2846	.	+	0	ID=BBa_S03721;Name=BBa_S03721
BBa_I715031	.	engineered_region	64	1629	.	+	0	ID=BBa_S03711;Name=BBa_S03711;Parent=BBa_S03721
BBa_I715031	.	engineered_region	64	897	.	+	0	ID=BBa_S03709;Name=BBa_S03709;Parent=BBa_S03711
BBa_I715031	.	CDS	82	897	.	+	0	ID=BBa_J31003;Name=BBa_J31003;Parent=BBa_S03709
BBa_I715031	.	CDS	82	897	.	+	0	ID=annotation1884996;Name=KanR Gene;Parent=BBa_J31003
BBa_I715031	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03709
BBa_I715031	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715031	.	engineered_region	906	1629	.	+	0	ID=BBa_I13502;Name=BBa_I13502;Parent=BBa_S03711
BBa_I715031	.	ribosome_entry_site	906	917	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_I715031	.	conserved_region	910	913	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715031	.	CDS	924	1604	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_I715031	.	CDS	924	1598	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_I715031	.	engineered_tag	1605	1629	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_I715031	.	engineered_region	1638	2846	.	+	0	ID=BBa_S03710;Name=BBa_S03710;Parent=BBa_S03721
BBa_I715031	.	CDS	1656	2846	.	+	0	ID=BBa_J31007;Name=BBa_J31007;Parent=BBa_S03710
BBa_I715031	.	primer_binding_site	1656	1679	.	+	0	ID=annotation1910571;Name=Fwd primer J31007;Parent=BBa_J31007
BBa_I715031	.	primer_binding_site	2827	2846	.	+	0	ID=annotation1910572;Name=Rev primer J31007;Parent=BBa_J31007
BBa_I715031	.	CDS	1656	2846	.	+	0	ID=annotation1885000;Name=tetA(C) forward;Parent=BBa_J31007
BBa_I715031	.	stop_codon	2844	2846	.	+	0	ID=annotation1910573;Name=stop;Parent=BBa_J31007
BBa_I715031	.	ribosome_entry_site	1638	1649	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03710
BBa_I715031	.	conserved_region	1642	1645	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I715031
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgagccatattcaacgggaaacgtcttgctccaggccgcgattaaattccaacatggatgctgatttatatgggta
taaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttg
tttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaattta
tgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccgggaaaac
agcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttg
cattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaata
acggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataa
gcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaa
ttaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcg
gtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtt
tcatttgatgctcgatgagtttttctaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatca
aagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaagg
tcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctg
tccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcc
cggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgca
agacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaacc
atgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaac
tgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctta
caaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggt
cgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatga
aatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtact
gccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttg
atgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctac
ttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccgg
catcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttc
gggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgc
atgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataa
gggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtc
gccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgagg
accgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagcctt
cgtcactggccccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctac
gtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgc
ccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttac
cagcctaacttcgatcattggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggca
tggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacct
aa