LOCUS       BBa_I715033             2137 bp    DNA     linear       02-May-2025
DEFINITION  lacIq reverse-RBS reverse-pLac-RBS-HinLVA-TT
ACCESSION   BBa_I715033
VERSION     BBa_I715033.1  
FEATURES             Location/Qualifiers
     CDS             1..1128
                     /label=BBa_I715032
     RBS             1137..1151
                     /label=Reverse RBS
     RBS             1137..1151
                     /label=BBa_J44001
     CDS             1160..1247
                     /label=end of LacI coding region (inactive)
     misc_feature    1160..1359
                     /label=BBa_R0010
     misc_feature    1248..1285
                     /label=CAP binding site
     promoter        1296..1301
                     /label=-35
     promoter        1320..1325
                     /label=-10
     misc_feature    1325..1359
                     /label=LacI binding site
     misc_feature    1332..1332
                     /label=start
     promoter        1160..1359
                     /label=BBa_R0010
     RBS             1368..1382
                     /label=RBS-1\Strong
     RBS             1375..1379
                     /label=RBS
     misc_feature    1368..1382
                     /label=BBa_B0030
     RBS             1368..1382
                     /label=BBa_B0030
     CDS             1389..1958
                     /label=Hin
     primer_bind     1389..1391
                     /label=Fwd primer J31001
     misc_feature    1959..1997
                     /label=LVA
     misc_feature    1998..2000
                     /label=Stop codon
     primer_bind     1939..2000
                     /label=Rev primer J31001
     CDS             1389..2000
                     /label=BBa_J31001
     stem_loop       2020..2063
                     /label=stem_loop
     misc_feature    2009..2088
                     /label=BBa_B0010
     terminator      2009..2088
                     /label=BBa_B0010
     stem_loop       2104..2123
                     /label=T7 TE
     misc_feature    2130..2130
                     /label=stop
     polyA_site      2124..2137
                     /label=polya
     misc_feature    2097..2137
                     /label=BBa_B0012
     terminator      2097..2137
                     /label=BBa_B0012
ORIGIN
        1 ttattaagct actaaagcgt agttttcgtc gtttgcagcc tgcccgcttt ccagtcggga
       61 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      121 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
      181 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
      241 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
      301 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
      361 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
      421 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
      481 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
      541 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
      601 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
      661 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
      721 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
      781 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
      841 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
      901 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
      961 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     1021 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     1081 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccatta ctagagtttc
     1141 tcctctttaa ttactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1201 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     1261 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     1321 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagatt aaagaggaga
     1381 aatactagat ggctactatt gggtatattc gggtgtcaac aattgaccaa aatatcgatt
     1441 tacagcgtaa tgcgcttacc agtgcaaatt gtgaccgcat ttttgaggac cgtatcagtg
     1501 gcaagattgc aaaccgcccc ggcctgaaac gagcgttaaa gtatgtaaat aaaggcgata
     1561 ctcttgtcgt ctggaaatta gacagactgg gccgcagcgt gaaaaacctg gtggcgttaa
     1621 tatcagaatt acatgaacgt ggagctcact tccattcttt aaccgatagt attgatacca
     1681 gtagcgcgat ggggcgattc ttttttcatg taatgtcagc actggccgag atggagcgag
     1741 aattaattgt cgagcgaacc cttgccggac tggctgccgc cagagcgcaa ggacgactgg
     1801 gagggcgccc tcgggcgatc aacagacatg aacaggaaca gattagtcgg ctattagaga
     1861 aaggccatcc tcggcagcaa ctagctatta tttttggtat tggcgtatct accttataca
     1921 gatattttcc ggcaagccgt ataaaaaaac gaatgaatag gcctgctgca aacgacgaaa
     1981 actacgcttt agtagcttaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2041 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2101 actggctcac cttcgggtgg gcctttctgc gtttata
//