##gff-version 3
##sequence-region BBa_I715033 1 2137
BBa_I715033	.	terminator	2009	2088	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I715033	.	stem_loop	2020	2063	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I715033	.	engineered_region	2009	2088	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I715033	.	CDS	1	1128	.	+	0	ID=BBa_I715032;Name=BBa_I715032
BBa_I715033	.	ribosome_entry_site	1368	1382	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I715033	.	engineered_region	1368	1382	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I715033	.	ribosome_entry_site	1375	1379	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I715033	.	ribosome_entry_site	1368	1382	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I715033	.	promoter	1160	1359	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I715033	.	non_covalent_binding_site	1325	1359	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I715033	.	promoter	1296	1301	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I715033	.	CDS	1160	1247	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I715033	.	start_codon	1332	1332	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I715033	.	non_covalent_binding_site	1248	1285	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I715033	.	promoter	1320	1325	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I715033	.	engineered_region	1160	1359	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I715033	.	terminator	2097	2137	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I715033	.	polyA_site	2124	2137	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I715033	.	stem_loop	2104	2123	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I715033	.	stop_codon	2130	2130	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I715033	.	engineered_region	2097	2137	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I715033	.	CDS	1389	2000	.	+	0	ID=BBa_J31001;Name=BBa_J31001
BBa_I715033	.	primer_binding_site	1939	2000	.	+	0	ID=annotation1910570;Name=Rev primer J31001;Parent=BBa_J31001
BBa_I715033	.	CDS	1389	1958	.	+	0	ID=annotation1910564;Name=Hin;Parent=BBa_J31001
BBa_I715033	.	primer_binding_site	1389	1391	.	+	0	ID=annotation1910565;Name=Fwd primer J31001;Parent=BBa_J31001
BBa_I715033	.	stop_codon	1998	2000	.	+	0	ID=annotation1910567;Name=Stop codon;Parent=BBa_J31001
BBa_I715033	.	tag	1959	1997	.	+	0	ID=annotation1910566;Name=LVA;Parent=BBa_J31001
BBa_I715033	.	ribosome_entry_site	1137	1151	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_I715033	.	ribosome_entry_site	1137	1151	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
>BBa_I715033
ttattaagctactaaagcgtagttttcgtcgtttgcagcctgcccgctttccagtcgggaaacctgtcgtgccagctgc
attaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgaga
cgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcag
gcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgag
atatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagca
tcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccg
ttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaactt
aatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcat
gggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttc
cacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcacc
gccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatt
taatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcc
cgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttttcgca
gaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacg
ttactggtttcacattcaccattactagagtttctcctctttaattactagagcaatacgcaaaccgcctctccccgcg
cgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatg
tgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcgg
ataacaatttcacacatactagagattaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaa
ttgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtgg
caagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaatta
gacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctt
taaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcg
agaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcg
atcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttg
gtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacga
cgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata