LOCUS       BBa_I715035              962 bp    DNA     linear       10-Dec-2025
DEFINITION  pLAC-RBS-GFP1-HixC-RFP2
ACCESSION   BBa_I715035
VERSION     BBa_I715035.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     misc_feature    230..232
                     /label=Start Codon
     CDS             230..700
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    230..700
                     /label=BBa_I715019
     misc_feature    709..734
                     /label=BBa_J44000
     misc_feature    743..743
                     /label=extra base (avoid frameshift)
     CDS             744..956
                     /label=Continue coding sequence
     misc_feature    957..961
                     /label=(two in tandem)
     CDS             743..962
                     /label=BBa_I715023
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcgtaaagg
      241 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      301 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      361 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      421 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      481 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      541 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      601 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      661 ctataactca cacaatgtat acatcatggc agacaaacaa tactagagtt atcaaaaacc
      721 atggtttttg ataatactag agtggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
      781 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
      841 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
      901 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
      961 aa
//