LOCUS       BBa_I715037             2513 bp    DNA     linear       29-Nov-2024
DEFINITION  HPP-B1
ACCESSION   BBa_I715037
VERSION     BBa_I715037.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     CDS             50..511
                     /label=Coding sequence
     CDS             50..511
                     /label=BBa_I715022
     misc_feature    520..545
                     /label=BBa_J44000
     misc_feature    554..554
                     /label=extra base to maintain reading frame
     CDS             554..797
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    798..803
                     /label=Stop Codons (two in tandem)
     misc_feature    554..803
                     /label=BBa_I715020
     misc_feature    816..819
                     /label=conserved
     RBS             812..823
                     /label=BBa_B0034
     misc_feature    830..832
                     /label=start
     CDS             830..1291
                     /label=Coding sequence
     CDS             830..1291
                     /label=BBa_I715022
     misc_feature    1300..1325
                     /label=BBa_J44000
     misc_feature    1334..1334
                     /label=extra base to maintain reading frame
     CDS             1334..1577
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    1578..1583
                     /label=Stop Codons (two in tandem)
     misc_feature    1334..1583
                     /label=BBa_I715020
     stem_loop       1603..1646
                     /label=stem_loop
     misc_feature    1592..1671
                     /label=BBa_B0010
     terminator      1592..1671
                     /label=BBa_B0010
     stem_loop       1687..1706
                     /label=T7 TE
     misc_feature    1713..1713
                     /label=stop
     polyA_site      1707..1720
                     /label=polya
     misc_feature    1680..1720
                     /label=BBa_B0012
     terminator      1680..1720
                     /label=BBa_B0012
     misc_feature    1729..1754
                     /label=BBa_J44000
     misc_feature    1763..1763
                     /label=extra base (avoid frameshift)
     CDS             1764..1976
                     /label=Continue coding sequence
     misc_feature    1977..1981
                     /label=(two in tandem)
     CDS             1763..1982
                     /label=BBa_I715023
     misc_feature    1995..1998
                     /label=conserved
     RBS             1991..2002
                     /label=BBa_B0034
     misc_feature    2009..2011
                     /label=Start Codon
     CDS             2009..2479
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    2009..2479
                     /label=BBa_I715019
     misc_feature    2488..2513
                     /label=BBa_J44000
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga ctactagagt tatcaaaaac catggttttt
      541 gataatacta gagtaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      781 tggatgaact atacaaataa taatactaga gaaagaggag aaatactaga tggcttcctc
      841 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      901 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      961 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
     1021 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
     1081 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
     1141 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
     1201 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
     1261 agcttccacc gaacgtatgt acccggaaga ctactagagt tatcaaaaac catggttttt
     1321 gataatacta gagtaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
     1381 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
     1441 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
     1501 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
     1561 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
     1621 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1681 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt atcaaaaacc
     1741 atggtttttg ataatactag agtggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1801 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1861 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1921 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1981 aatactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt
     2041 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
     2101 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
     2161 aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt
     2221 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg
     2281 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
     2341 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
     2401 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
     2461 catcatggca gacaaacaat actagagtta tcaaaaacca tggtttttga taa
//