BBa_I715037
type: ComponentDefinition
persistent identity: BBa I715037
display id: BBa_I715037
version: 1
was generated by: iGEM to SBOL conversion
description: HPP-B1
created: 2007-07-28T11:00:00Z
modified: 2015-08-31T04:07:49Z
mutable provenance: Combine parts BBa_S03763 and BBa_S03764.
owned by: james
owned by: myers
mutable description: This is the first computer that we will build. In its starting orientation there are no phenotypes expressed. There is the possibility of a false positive. There is only one true positive.
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1620
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Alternatively, we may try building it in fewer steps doing a triple ligation.
star: true
creator: Andrew Martens
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I715023
component: BBa_J44000
component: BBa_I715019
component: BBa_B0010
component: BBa_I715020
component: BBa_B0012
component: BBa_I715022
component: BBa_R0085
sequence annotation: BBa_R0085
sequence annotation: BBa_B0034
sequence annotation: BBa_I715022
sequence annotation: BBa_J44000
sequence annotation: BBa_I715020
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I715023
sequence annotation: BBa_I715019
sequence: https://synbiohub.org/public/igem/BBa_I715037_sequence/1

BBa_B0034
type: Component
persistent identity: component1940156
display id: component1940156
version: 1
top level: BBa_I715037
definition: BBa_B0034
access: public

BBa_I715023
type: Component
persistent identity: component1940154
display id: component1940154
version: 1
top level: BBa_I715037
definition: RFP2
access: public

BBa_J44000
type: Component
persistent identity: component1940160
display id: component1940160
version: 1
top level: BBa_I715037
definition: hixC
access: public

BBa_I715019
type: Component
persistent identity: component1940159
display id: component1940159
version: 1
top level: BBa_I715037
definition: GFP1
access: public

BBa_B0010
type: Component
persistent identity: component1940145
display id: component1940145
version: 1
top level: BBa_I715037
definition: BBa_B0010
access: public

BBa_I715020
type: Component
persistent identity: component1940144
display id: component1940144
version: 1
top level: BBa_I715037
definition: GFP2
access: public

BBa_J44000
type: Component
persistent identity: component1940151
display id: component1940151
version: 1
top level: BBa_I715037
definition: hixC
access: public

BBa_B0012
type: Component
persistent identity: component1940147
display id: component1940147
version: 1
top level: BBa_I715037
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1940137
display id: component1940137
version: 1
top level: BBa_I715037
definition: BBa_B0034
access: public

BBa_I715020
type: Component
persistent identity: component1940135
display id: component1940135
version: 1
top level: BBa_I715037
definition: GFP2
access: public

BBa_J44000
type: Component
persistent identity: component1940141
display id: component1940141
version: 1
top level: BBa_I715037
definition: hixC
access: public

BBa_I715022
type: Component
persistent identity: component1940140
display id: component1940140
version: 1
top level: BBa_I715037
definition: RFP1
access: public

BBa_B0034
type: Component
persistent identity: component1940128
display id: component1940128
version: 1
top level: BBa_I715037
definition: BBa_B0034
access: public

BBa_R0085
type: Component
persistent identity: component1940126
display id: component1940126
version: 1
top level: BBa_I715037
definition: BBa_R0085
access: public

BBa_J44000
type: Component
persistent identity: component1940132
display id: component1940132
version: 1
top level: BBa_I715037
definition: hixC
access: public

BBa_I715022
type: Component
persistent identity: component1940131
display id: component1940131
version: 1
top level: BBa_I715037
definition: RFP1
access: public

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1940126
display id: annotation1940126
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940126/range1940126/1
component: BBa_R0085

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type: Range
persistent identity: range1940126
display id: range1940126
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 1
end: 23
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940128
display id: annotation1940128
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940128/range1940128/1
component: BBa_B0034

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type: Range
persistent identity: range1940128
display id: range1940128
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 32
end: 43
orientation: inline

BBa_I715022
type: SequenceAnnotation
persistent identity: annotation1940131
display id: annotation1940131
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940131/range1940131/1
component: BBa_I715022

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type: Range
persistent identity: range1940131
display id: range1940131
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 50
end: 511
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1940132
display id: annotation1940132
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940132/range1940132/1
component: BBa_J44000

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type: Range
persistent identity: range1940132
display id: range1940132
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 520
end: 545
orientation: inline

BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1940135
display id: annotation1940135
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940135/range1940135/1
component: BBa_I715020

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type: Range
persistent identity: range1940135
display id: range1940135
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 554
end: 803
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940137
display id: annotation1940137
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940137/range1940137/1
component: BBa_B0034

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type: Range
persistent identity: range1940137
display id: range1940137
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 812
end: 823
orientation: inline

BBa_I715022
type: SequenceAnnotation
persistent identity: annotation1940140
display id: annotation1940140
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940140/range1940140/1
component: BBa_I715022

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type: Range
persistent identity: range1940140
display id: range1940140
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 830
end: 1291
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1940141
display id: annotation1940141
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940141/range1940141/1
component: BBa_J44000

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type: Range
persistent identity: range1940141
display id: range1940141
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 1300
end: 1325
orientation: inline

BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1940144
display id: annotation1940144
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940144/range1940144/1
component: BBa_I715020

https://synbiohub.org/public/igem/BBa_I715037/annotation1940144/range1940144/1
type: Range
persistent identity: range1940144
display id: range1940144
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 1334
end: 1583
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1940145
display id: annotation1940145
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940145/range1940145/1
component: BBa_B0010

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type: Range
persistent identity: range1940145
display id: range1940145
version: 1
top level: BBa_I715037
direction: direction/forward
start: 1592
end: 1671
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1940147
display id: annotation1940147
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940147/range1940147/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715037/annotation1940147/range1940147/1
type: Range
persistent identity: range1940147
display id: range1940147
version: 1
top level: BBa_I715037
direction: direction/forward
start: 1680
end: 1720
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1940151
display id: annotation1940151
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940151/range1940151/1
component: BBa_J44000

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type: Range
persistent identity: range1940151
display id: range1940151
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 1729
end: 1754
orientation: inline

BBa_I715023
type: SequenceAnnotation
persistent identity: annotation1940154
display id: annotation1940154
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940154/range1940154/1
component: BBa_I715023

https://synbiohub.org/public/igem/BBa_I715037/annotation1940154/range1940154/1
type: Range
persistent identity: range1940154
display id: range1940154
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 1763
end: 1982
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1940156
display id: annotation1940156
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940156/range1940156/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715037/annotation1940156/range1940156/1
type: Range
persistent identity: range1940156
display id: range1940156
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 1991
end: 2002
orientation: inline

BBa_I715019
type: SequenceAnnotation
persistent identity: annotation1940159
display id: annotation1940159
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940159/range1940159/1
component: BBa_I715019

https://synbiohub.org/public/igem/BBa_I715037/annotation1940159/range1940159/1
type: Range
persistent identity: range1940159
display id: range1940159
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 2009
end: 2479
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1940160
display id: annotation1940160
version: 1
top level: BBa_I715037
location: https://synbiohub.org/public/igem/BBa_I715037/annotation1940160/range1940160/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715037/annotation1940160/range1940160/1
type: Range
persistent identity: range1940160
display id: range1940160
version: 1
top level: BBa_I715037
direction: direction/reverse
start: 2488
end: 2513
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

RFP1
type: ComponentDefinition
persistent identity: BBa I715022
display id: BBa_I715022
version: 1
was generated by: iGEM to SBOL conversion
description: Amino Portion of RFP
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:43:58Z
mutable provenance: adf
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: af
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
sample status: In stock
status: status/Available
bookmark: false
mutable notes: afd
star: true
creator: Andrew Martens
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I715022_sequence/1

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1935381
display id: annotation1935381
version: 1
top level: RFP1
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
type: Range
persistent identity: range1935381
display id: range1935381
version: 1
top level: RFP1
direction: direction/forward
start: 1
end: 462
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1935380
display id: annotation1935380
version: 1
top level: RFP1
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
type: Range
persistent identity: range1935380
display id: range1935380
version: 1
top level: RFP1
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP1
type: ComponentDefinition
persistent identity: BBa I715019
display id: BBa_I715019
version: 1
was generated by: iGEM to SBOL conversion
description: Amino Half of GFP (aka GFP1)
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
mutable provenance: A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: A
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: A
star: true
creator: Will DeLoache
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Coding Amino Portion GFP (aka GFP1)
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_I715019_sequence/1

Coding Amino Portion GFP (aka GFP1)
type: SequenceAnnotation
persistent identity: annotation1935357
display id: annotation1935357
version: 1
top level: GFP1
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1
type: Range
persistent identity: range1935357
display id: range1935357
version: 1
top level: GFP1
direction: direction/forward
start: 1
end: 471
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1935356
display id: annotation1935356
version: 1
top level: GFP1
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1
type: Range
persistent identity: range1935356
display id: range1935356
version: 1
top level: GFP1
direction: direction/forward
start: 1
end: 3
orientation: inline

GFP2
type: ComponentDefinition
persistent identity: BBa I715020
display id: BBa_I715020
version: 1
was generated by: iGEM to SBOL conversion
description: Carboxyl Half of GFP (aka GFP2)
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
mutable provenance: A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: A
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: A
star: true
creator: Will DeLoache
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Coding Carboxyl half of GFP (aka GFP2)
sequence annotation: extra base to maintain reading frame
sequence annotation: Stop Codons (two in tandem)
sequence: https://synbiohub.org/public/igem/BBa_I715020_sequence/1

Coding Carboxyl half of GFP (aka GFP2)
type: SequenceAnnotation
persistent identity: annotation1935361
display id: annotation1935361
version: 1
top level: GFP2
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1
type: Range
persistent identity: range1935361
display id: range1935361
version: 1
top level: GFP2
direction: direction/forward
start: 1
end: 244
orientation: inline

extra base to maintain reading frame
type: SequenceAnnotation
persistent identity: annotation1935360
display id: annotation1935360
version: 1
top level: GFP2
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1
type: Range
persistent identity: range1935360
display id: range1935360
version: 1
top level: GFP2
direction: direction/forward
start: 1
end: 1
orientation: inline

Stop Codons (two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935362
display id: annotation1935362
version: 1
top level: GFP2
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1
type: Range
persistent identity: range1935362
display id: range1935362
version: 1
top level: GFP2
direction: direction/forward
start: 245
end: 250
orientation: inline

RFP2
type: ComponentDefinition
persistent identity: BBa I715023
display id: BBa_I715023
version: 1
was generated by: iGEM to SBOL conversion
description: Carboxyl portion of RFP
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:44:49Z
mutable provenance: Sequence from part BBa_E1010.
owned by: james
owned by: myers
mutable description: The second portion of the RFP gene, after the HixC insertion point.
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Used web tool.
star: true
creator: Andrew Martens
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: extra base (avoid frameshift)
sequence annotation: Continue coding sequence
sequence annotation: (two in tandem)
sequence: https://synbiohub.org/public/igem/BBa_I715023_sequence/1

extra base (avoid frameshift)
type: SequenceAnnotation
persistent identity: annotation1935382
display id: annotation1935382
version: 1
top level: RFP2
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1
type: Range
persistent identity: range1935382
display id: range1935382
version: 1
top level: RFP2
direction: direction/forward
start: 1
end: 1
orientation: inline

Continue coding sequence
type: SequenceAnnotation
persistent identity: annotation1935383
display id: annotation1935383
version: 1
top level: RFP2
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1
type: Range
persistent identity: range1935383
display id: range1935383
version: 1
top level: RFP2
direction: direction/forward
start: 2
end: 214
orientation: inline

(two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935384
display id: annotation1935384
version: 1
top level: RFP2
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1
type: Range
persistent identity: range1935384
display id: range1935384
version: 1
top level: RFP2
direction: direction/forward
start: 215
end: 219
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: iGEM to SBOL conversion
description: hixC binding site for Salmonella typhimurium Hin recombinase
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
star: true
creator: Missouri Western and Davidson Groups, Todd Eckdahl
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1

https://synbiohub.org/public/igem/BBa_I715037_sequence/1
type: Sequence
persistent identity: BBa I715037 sequence
display id: BBa_I715037_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagactactagagttatcaaaaaccatggtttttgataatactagagtaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagactactagagttatcaaaaaccatggtttttgataatactagagtaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatcaaaaaccatggtttttgataatactagagtggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagagaaagaggaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715022_sequence/1
type: Sequence
persistent identity: BBa I715022 sequence
display id: BBa_I715022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715019_sequence/1
type: Sequence
persistent identity: BBa I715019 sequence
display id: BBa_I715019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715020_sequence/1
type: Sequence
persistent identity: BBa I715020 sequence
display id: BBa_I715020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715023_sequence/1
type: Sequence
persistent identity: BBa I715023 sequence
display id: BBa_I715023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44000_sequence/1
type: Sequence
persistent identity: BBa J44000 sequence
display id: BBa_J44000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z