LOCUS       BBa_I715040             1681 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac-RBS-RFP-RBS-GFP
ACCESSION   BBa_I715040
VERSION     BBa_I715040.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..901
                     /label=mrfp1
     misc_feature    908..932
                     /label=Help:Barcodes
     CDS             227..907
                     /label=BBa_E1010
     misc_feature    209..932
                     /label=BBa_I13502
     misc_feature    1..932
                     /label=BBa_I715039
     RBS             941..955
                     /label=RBS-1\Strong
     RBS             948..952
                     /label=RBS
     misc_feature    941..955
                     /label=BBa_B0030
     RBS             941..955
                     /label=BBa_B0030
     CDS             962..1681
                     /label=GFP protein
     CDS             962..1681
                     /label=BBa_E0040
     misc_feature    941..1681
                     /label=BBa_E5500
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gctactagag attaaagagg agaaatacta
      961 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
     1021 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     1081 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
     1141 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
     1201 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     1261 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
     1321 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
     1381 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
     1441 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
     1501 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     1561 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
     1621 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
     1681 a
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