LOCUS       BBa_I715043             2132 bp    DNA     linear       23-Dec-2024
DEFINITION  HPP-A1
ACCESSION   BBa_I715043
VERSION     BBa_I715043.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     CDS             50..511
                     /label=Coding sequence
     CDS             50..511
                     /label=BBa_I715022
     misc_feature    520..545
                     /label=BBa_J44000
     misc_feature    554..554
                     /label=extra base (avoid frameshift)
     CDS             555..767
                     /label=Continue coding sequence
     misc_feature    768..772
                     /label=(two in tandem)
     CDS             554..773
                     /label=BBa_I715023
     misc_feature    786..789
                     /label=conserved
     RBS             782..793
                     /label=BBa_B0034
     misc_feature    800..802
                     /label=Start Codon
     CDS             800..1270
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    800..1270
                     /label=BBa_I715019
     misc_feature    1279..1304
                     /label=BBa_J44000
     misc_feature    1313..1313
                     /label=extra base (avoid frameshift)
     CDS             1314..1526
                     /label=Continue coding sequence
     misc_feature    1527..1531
                     /label=(two in tandem)
     CDS             1313..1532
                     /label=BBa_I715023
     stem_loop       1552..1595
                     /label=stem_loop
     misc_feature    1541..1620
                     /label=BBa_B0010
     terminator      1541..1620
                     /label=BBa_B0010
     stem_loop       1636..1655
                     /label=T7 TE
     misc_feature    1662..1662
                     /label=stop
     polyA_site      1656..1669
                     /label=polya
     misc_feature    1629..1669
                     /label=BBa_B0012
     terminator      1629..1669
                     /label=BBa_B0012
     misc_feature    1678..1703
                     /label=BBa_J44000
     misc_feature    1712..1712
                     /label=extra base to maintain reading frame
     CDS             1712..1955
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    1956..1961
                     /label=Stop Codons (two in tandem)
     misc_feature    1712..1961
                     /label=BBa_I715020
     stem_loop       1981..2024
                     /label=stem_loop
     misc_feature    1970..2049
                     /label=BBa_B0010
     terminator      1970..2049
                     /label=BBa_B0010
     stem_loop       2065..2084
                     /label=T7 TE
     misc_feature    2091..2091
                     /label=stop
     polyA_site      2085..2098
                     /label=polya
     misc_feature    2058..2098
                     /label=BBa_B0012
     terminator      2058..2098
                     /label=BBa_B0012
     misc_feature    2107..2132
                     /label=BBa_J44000
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga ctactagagt tatcaaaaac catggttttt
      541 gataatacta gagtggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
      601 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
      661 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
      721 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taatactaga
      781 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      841 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      901 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      961 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
     1021 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1081 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1141 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1201 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1261 agacaaacaa tactagagtt atcaaaaacc atggtttttg ataatactag agtggtgctc
     1321 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag
     1381 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca
     1441 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg
     1501 ctgaaggtcg tcactccacc ggtgcttaat aatactagag ccaggcatca aataaaacga
     1561 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1621 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtta
     1681 tcaaaaacca tggtttttga taatactaga gtaagaatgg aatcaaagtt aacttcaaaa
     1741 ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc
     1801 caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc
     1861 tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg
     1921 ctgggattac acatggcatg gatgaactat acaaataata atactagagc caggcatcaa
     1981 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2041 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     2101 ctagagttat caaaaaccat ggtttttgat aa
//