LOCUS       BBa_I715044             2132 bp    DNA     linear       08-Jun-2024
DEFINITION  HPP-A2
ACCESSION   BBa_I715044
VERSION     BBa_I715044.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     CDS             50..511
                     /label=Coding sequence
     CDS             50..511
                     /label=BBa_I715022
     misc_feature    520..545
                     /label=BBa_J44000
     misc_feature    554..554
                     /label=extra base to maintain reading frame
     CDS             554..797
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    798..803
                     /label=Stop Codons (two in tandem)
     misc_feature    554..803
                     /label=BBa_I715020
     stem_loop       823..866
                     /label=stem_loop
     misc_feature    812..891
                     /label=BBa_B0010
     terminator      812..891
                     /label=BBa_B0010
     stem_loop       907..926
                     /label=T7 TE
     misc_feature    933..933
                     /label=stop
     polyA_site      927..940
                     /label=polya
     misc_feature    900..940
                     /label=BBa_B0012
     terminator      900..940
                     /label=BBa_B0012
     misc_feature    949..974
                     /label=BBa_J44000
     misc_feature    983..983
                     /label=extra base (avoid frameshift)
     CDS             984..1196
                     /label=Continue coding sequence
     misc_feature    1197..1201
                     /label=(two in tandem)
     CDS             983..1202
                     /label=BBa_I715023
     misc_feature    1215..1218
                     /label=conserved
     RBS             1211..1222
                     /label=BBa_B0034
     misc_feature    1229..1231
                     /label=Start Codon
     CDS             1229..1699
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    1229..1699
                     /label=BBa_I715019
     misc_feature    1708..1733
                     /label=BBa_J44000
     misc_feature    1742..1742
                     /label=extra base (avoid frameshift)
     CDS             1743..1955
                     /label=Continue coding sequence
     misc_feature    1956..1960
                     /label=(two in tandem)
     CDS             1742..1961
                     /label=BBa_I715023
     stem_loop       1981..2024
                     /label=stem_loop
     misc_feature    1970..2049
                     /label=BBa_B0010
     terminator      1970..2049
                     /label=BBa_B0010
     stem_loop       2065..2084
                     /label=T7 TE
     misc_feature    2091..2091
                     /label=stop
     polyA_site      2085..2098
                     /label=polya
     misc_feature    2058..2098
                     /label=BBa_B0012
     terminator      2058..2098
                     /label=BBa_B0012
     misc_feature    2107..2132
                     /label=BBa_J44000
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga ctactagagt tatcaaaaac catggttttt
      541 gataatacta gagtaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag
      601 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg
      661 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg
      721 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca
      781 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag
      841 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      901 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt atcaaaaacc
      961 atggtttttg ataatactag agtggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
     1021 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
     1081 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
     1141 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
     1201 aatactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt
     1261 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg
     1321 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg
     1381 aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt
     1441 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg
     1501 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga
     1561 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa
     1621 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata
     1681 catcatggca gacaaacaat actagagtta tcaaaaacca tggtttttga taatactaga
     1741 gtggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
     1801 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
     1861 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
     1921 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata atactagagc caggcatcaa
     1981 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     2041 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     2101 ctagagttat caaaaaccat ggtttttgat aa
//