LOCUS       BBa_I715046             1733 bp    DNA     linear       22-Nov-2024
DEFINITION  T7pro-RBS-RFP1-HixC-RFP2-RBS-GFP1-HixC-GFP2-TT-HixC
ACCESSION   BBa_I715046
VERSION     BBa_I715046.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=start
     CDS             50..511
                     /label=Coding sequence
     CDS             50..511
                     /label=BBa_I715022
     misc_feature    520..545
                     /label=BBa_J44000
     misc_feature    554..554
                     /label=extra base (avoid frameshift)
     CDS             555..767
                     /label=Continue coding sequence
     misc_feature    768..772
                     /label=(two in tandem)
     CDS             554..773
                     /label=BBa_I715023
     misc_feature    786..789
                     /label=conserved
     RBS             782..793
                     /label=BBa_B0034
     misc_feature    800..802
                     /label=Start Codon
     CDS             800..1270
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    800..1270
                     /label=BBa_I715019
     misc_feature    1279..1304
                     /label=BBa_J44000
     misc_feature    1313..1313
                     /label=extra base to maintain reading frame
     CDS             1313..1556
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    1557..1562
                     /label=Stop Codons (two in tandem)
     misc_feature    1313..1562
                     /label=BBa_I715020
     stem_loop       1582..1625
                     /label=stem_loop
     misc_feature    1571..1650
                     /label=BBa_B0010
     terminator      1571..1650
                     /label=BBa_B0010
     stem_loop       1666..1685
                     /label=T7 TE
     misc_feature    1692..1692
                     /label=stop
     polyA_site      1686..1699
                     /label=polya
     misc_feature    1659..1699
                     /label=BBa_B0012
     terminator      1659..1699
                     /label=BBa_B0012
     misc_feature    1708..1733
                     /label=BBa_J44000
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga ctactagagt tatcaaaaac catggttttt
      541 gataatacta gagtggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
      601 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
      661 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
      721 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taatactaga
      781 gaaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
      841 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      901 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      961 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
     1021 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1081 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1141 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1201 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1261 agacaaacaa tactagagtt atcaaaaacc atggtttttg ataatactag agtaagaatg
     1321 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     1381 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1441 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1501 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1561 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1621 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1681 gggcctttct gcgtttatat actagagtta tcaaaaacca tggtttttga taa
//