LOCUS BBa_I715049 3118 bp DNA linear 26-Dec-2024 DEFINITION HPP-A1 + Hin ACCESSION BBa_I715049 VERSION BBa_I715049.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..799 /label=Hin primer_bind 230..232 /label=Fwd primer J31001 misc_feature 800..838 /label=LVA misc_feature 839..841 /label=Stop codon primer_bind 780..841 /label=Rev primer J31001 CDS 230..841 /label=BBa_J31001 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 misc_feature 1004..1004 /label=Transcription Start Site misc_feature 987..997 /label=Polymerase Binding Region misc_feature 998..1009 /label=Initiation Region promoter 987..1009 /label=BBa_R0085 misc_feature 1022..1025 /label=conserved RBS 1018..1029 /label=BBa_B0034 misc_feature 1036..1038 /label=start CDS 1036..1497 /label=Coding sequence CDS 1036..1497 /label=BBa_I715022 misc_feature 1506..1531 /label=BBa_J44000 misc_feature 1540..1540 /label=extra base (avoid frameshift) CDS 1541..1753 /label=Continue coding sequence misc_feature 1754..1758 /label=(two in tandem) CDS 1540..1759 /label=BBa_I715023 misc_feature 1772..1775 /label=conserved RBS 1768..1779 /label=BBa_B0034 misc_feature 1786..1788 /label=Start Codon CDS 1786..2256 /label=Coding Amino Portion GFP (aka GFP1) misc_feature 1786..2256 /label=BBa_I715019 misc_feature 2265..2290 /label=BBa_J44000 misc_feature 2299..2299 /label=extra base (avoid frameshift) CDS 2300..2512 /label=Continue coding sequence misc_feature 2513..2517 /label=(two in tandem) CDS 2299..2518 /label=BBa_I715023 stem_loop 2538..2581 /label=stem_loop misc_feature 2527..2606 /label=BBa_B0010 terminator 2527..2606 /label=BBa_B0010 stem_loop 2622..2641 /label=T7 TE misc_feature 2648..2648 /label=stop polyA_site 2642..2655 /label=polya misc_feature 2615..2655 /label=BBa_B0012 terminator 2615..2655 /label=BBa_B0012 misc_feature 2664..2689 /label=BBa_J44000 misc_feature 2698..2698 /label=extra base to maintain reading frame CDS 2698..2941 /label=Coding Carboxyl half of GFP (aka GFP2) misc_feature 2942..2947 /label=Stop Codons (two in tandem) misc_feature 2698..2947 /label=BBa_I715020 stem_loop 2967..3010 /label=stem_loop misc_feature 2956..3035 /label=BBa_B0010 terminator 2956..3035 /label=BBa_B0010 stem_loop 3051..3070 /label=T7 TE misc_feature 3077..3077 /label=stop polyA_site 3071..3084 /label=polya misc_feature 3044..3084 /label=BBa_B0012 terminator 3044..3084 /label=BBa_B0012 misc_feature 3093..3118 /label=BBa_J44000 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggctactat 241 tgggtatatt cgggtgtcaa caattgacca aaatatcgat ttacagcgta atgcgcttac 301 cagtgcaaat tgtgaccgca tttttgagga ccgtatcagt ggcaagattg caaaccgccc 361 cggcctgaaa cgagcgttaa agtatgtaaa taaaggcgat actcttgtcg tctggaaatt 421 agacagactg ggccgcagcg tgaaaaacct ggtggcgtta atatcagaat tacatgaacg 481 tggagctcac ttccattctt taaccgatag tattgatacc agtagcgcga tggggcgatt 541 cttttttcat gtaatgtcag cactggccga gatggagcga gaattaattg tcgagcgaac 601 ccttgccgga ctggctgccg ccagagcgca aggacgactg ggagggcgcc ctcgggcgat 661 caacagacat gaacaggaac agattagtcg gctattagag aaaggccatc ctcggcagca 721 actagctatt atttttggta ttggcgtatc taccttatac agatattttc cggcaagccg 781 tataaaaaaa cgaatgaata ggcctgctgc aaacgacgaa aactacgctt tagtagctta 841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagagtaat acgactcact atagggagat actagagaaa 1021 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa 1081 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt 1141 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg 1201 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac 1261 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt 1321 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac 1381 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt 1441 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagactac 1501 tagagttatc aaaaaccatg gtttttgata atactagagt ggtgctctga aaggtgaaat 1561 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta 1621 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat 1681 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca 1741 ctccaccggt gcttaataat actagagaaa gaggagaaat actagatgcg taaaggagaa 1801 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac 1861 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa 1921 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt 1981 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag 2041 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac 2101 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta 2161 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat 2221 aactcacaca atgtatacat catggcagac aaacaatact agagttatca aaaaccatgg 2281 tttttgataa tactagagtg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa 2341 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca 2401 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta 2461 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataata 2521 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 2581 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2641 ctttctgcgt ttatatacta gagttatcaa aaaccatggt ttttgataat actagagtaa 2701 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact 2761 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 2821 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat 2881 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa 2941 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3001 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 3061 cgggtgggcc tttctgcgtt tatatactag agttatcaaa aaccatggtt tttgataa // LOCUS BBa_I715050 3118 bp DNA linear 26-Dec-2024 DEFINITION Hin + pT7 -> RFP1 + GFP1 medium initial orientation ACCESSION BBa_I715050 VERSION BBa_I715050.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..799 /label=Hin primer_bind 230..232 /label=Fwd primer J31001 misc_feature 800..838 /label=LVA misc_feature 839..841 /label=Stop codon primer_bind 780..841 /label=Rev primer J31001 CDS 230..841 /label=BBa_J31001 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 misc_feature 1004..1004 /label=Transcription Start Site misc_feature 987..997 /label=Polymerase Binding Region misc_feature 998..1009 /label=Initiation Region promoter 987..1009 /label=BBa_R0085 misc_feature 1022..1025 /label=conserved RBS 1018..1029 /label=BBa_B0034 misc_feature 1036..1038 /label=start CDS 1036..1497 /label=Coding sequence CDS 1036..1497 /label=BBa_I715022 misc_feature 1506..1531 /label=BBa_J44000 misc_feature 1540..1540 /label=extra base (avoid frameshift) CDS 1541..1753 /label=Continue coding sequence misc_feature 1754..1758 /label=(two in tandem) CDS 1540..1759 /label=BBa_I715023 misc_feature 1772..1775 /label=conserved RBS 1768..1779 /label=BBa_B0034 misc_feature 1786..1788 /label=Start Codon CDS 1786..2256 /label=Coding Amino Portion GFP (aka GFP1) misc_feature 1786..2256 /label=BBa_I715019 misc_feature 2265..2290 /label=BBa_J44000 misc_feature 2299..2299 /label=extra base (avoid frameshift) CDS 2300..2512 /label=Continue coding sequence misc_feature 2513..2517 /label=(two in tandem) CDS 2299..2518 /label=BBa_I715023 stem_loop 2538..2581 /label=stem_loop misc_feature 2527..2606 /label=BBa_B0010 terminator 2527..2606 /label=BBa_B0010 stem_loop 2622..2641 /label=T7 TE misc_feature 2648..2648 /label=stop polyA_site 2642..2655 /label=polya misc_feature 2615..2655 /label=BBa_B0012 terminator 2615..2655 /label=BBa_B0012 misc_feature 2664..2689 /label=BBa_J44000 misc_feature 2698..2698 /label=extra base to maintain reading frame CDS 2698..2941 /label=Coding Carboxyl half of GFP (aka GFP2) misc_feature 2942..2947 /label=Stop Codons (two in tandem) misc_feature 2698..2947 /label=BBa_I715020 stem_loop 2967..3010 /label=stem_loop misc_feature 2956..3035 /label=BBa_B0010 terminator 2956..3035 /label=BBa_B0010 stem_loop 3051..3070 /label=T7 TE misc_feature 3077..3077 /label=stop polyA_site 3071..3084 /label=polya misc_feature 3044..3084 /label=BBa_B0012 terminator 3044..3084 /label=BBa_B0012 misc_feature 3093..3118 /label=BBa_J44000 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggctactat 241 tgggtatatt cgggtgtcaa caattgacca aaatatcgat ttacagcgta atgcgcttac 301 cagtgcaaat tgtgaccgca tttttgagga ccgtatcagt ggcaagattg caaaccgccc 361 cggcctgaaa cgagcgttaa agtatgtaaa taaaggcgat actcttgtcg tctggaaatt 421 agacagactg ggccgcagcg tgaaaaacct ggtggcgtta atatcagaat tacatgaacg 481 tggagctcac ttccattctt taaccgatag tattgatacc agtagcgcga tggggcgatt 541 cttttttcat gtaatgtcag cactggccga gatggagcga gaattaattg tcgagcgaac 601 ccttgccgga ctggctgccg ccagagcgca aggacgactg ggagggcgcc ctcgggcgat 661 caacagacat gaacaggaac agattagtcg gctattagag aaaggccatc ctcggcagca 721 actagctatt atttttggta ttggcgtatc taccttatac agatattttc cggcaagccg 781 tataaaaaaa cgaatgaata ggcctgctgc aaacgacgaa aactacgctt tagtagctta 841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagagtaat acgactcact atagggagat actagagaaa 1021 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa 1081 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt 1141 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg 1201 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac 1261 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt 1321 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac 1381 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt 1441 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagactac 1501 tagagttatc aaaaaccatg gtttttgata atactagagt ggtgctctga aaggtgaaat 1561 caaaatgcgt ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta 1621 catggctaaa aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat 1681 cacctcccac aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca 1741 ctccaccggt gcttaataat actagagaaa gaggagaaat actagatgcg taaaggagaa 1801 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac 1861 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa 1921 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt 1981 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag 2041 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac 2101 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta 2161 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat 2221 aactcacaca atgtatacat catggcagac aaacaatact agagttatca aaaaccatgg 2281 tttttgataa tactagagtg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa 2341 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca 2401 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta 2461 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataata 2521 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 2581 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2641 ctttctgcgt ttatatacta gagttatcaa aaaccatggt ttttgataat actagagtaa 2701 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact 2761 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa 2821 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat 2881 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa 2941 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3001 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 3061 cgggtgggcc tttctgcgtt tatatactag agttatcaaa aaccatggtt tttgataa //