BBa_I715050
type: ComponentDefinition
persistent identity: BBa I715050
display id: BBa_I715050
version: 1
was generated by: iGEM to SBOL conversion
description: Hin + pT7 -> RFP1 + GFP1 medium initial orientation
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:50Z
mutable provenance: Ligation of existing parts
owned by: james
owned by: myers
mutable description: Part is composed of a Hin recombinase expression casette driven by pLac and series of flippable units that include halves of GFP and RFP: T7prom-RBS-RFP1-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC-GFP2-TT-hixC. The corresponding part without Hin does not glow. With Hin, most colonies do not glow, but green and red colonies appear. Evidence suggests that this is a result of Hin-mediated recombination.
discontinued: true
dominant: false
experience: experience/None
group u list: _120_
m user id: 0
owner id: 1858
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: None
star: false
is replaced by: BBa_I715049
creator: Amanda Valencia
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_B0030
component: BBa_R0010
component: BBa_B0010
component: BBa_J31001
component: BBa_I715023
component: BBa_J44000
component: BBa_I715019
component: BBa_B0034
component: BBa_R0085
component: BBa_B0012
component: BBa_I715022
component: BBa_I715020
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_J31001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0085
sequence annotation: BBa_B0034
sequence annotation: BBa_I715022
sequence annotation: BBa_J44000
sequence annotation: BBa_I715023
sequence annotation: BBa_I715019
sequence annotation: BBa_I715020
sequence: https://synbiohub.org/public/igem/BBa_I715050_sequence/1

BBa_B0030
type: Component
persistent identity: component1954229
display id: component1954229
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definition: BBa_B0030
access: public

BBa_R0010
type: Component
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display id: component1954221
version: 1
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definition: LacI
access: public

BBa_B0010
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definition: BBa_B0010
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BBa_J31001
type: Component
persistent identity: component1954236
display id: component1954236
version: 1
top level: BBa_I715050
definition: Hin LVA
access: public

BBa_I715023
type: Component
persistent identity: component1954255
display id: component1954255
version: 1
top level: BBa_I715050
definition: RFP2
access: public

BBa_J44000
type: Component
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display id: component1954252
version: 1
top level: BBa_I715050
definition: hixC
access: public

BBa_I715019
type: Component
persistent identity: component1954260
display id: component1954260
version: 1
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definition: GFP1
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BBa_B0034
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BBa_R0085
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BBa_B0012
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BBa_I715022
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display id: component1954251
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BBa_B0034
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BBa_I715020
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version: 1
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definition: GFP2
access: public

BBa_J44000
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definition: hixC
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BBa_J44000
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BBa_B0012
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BBa_B0010
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BBa_I715023
type: Component
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display id: component1954264
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definition: RFP2
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BBa_J44000
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definition: hixC
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BBa_B0012
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BBa_B0010
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BBa_R0010
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component: BBa_R0010

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BBa_B0030
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BBa_J31001
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BBa_B0012
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BBa_R0085
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BBa_B0034
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top level: BBa_I715050
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component: BBa_B0034

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BBa_I715022
type: SequenceAnnotation
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display id: annotation1954251
version: 1
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location: https://synbiohub.org/public/igem/BBa_I715050/annotation1954251/range1954251/1
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BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954252
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version: 1
top level: BBa_I715050
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BBa_I715023
type: SequenceAnnotation
persistent identity: annotation1954255
display id: annotation1954255
version: 1
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BBa_B0034
type: SequenceAnnotation
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display id: annotation1954257
version: 1
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BBa_I715019
type: SequenceAnnotation
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version: 1
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BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954261
display id: annotation1954261
version: 1
top level: BBa_I715050
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component: BBa_J44000

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persistent identity: range1954261
display id: range1954261
version: 1
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BBa_I715023
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display id: annotation1954264
version: 1
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BBa_B0010
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BBa_B0012
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BBa_J44000
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BBa_I715020
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BBa_B0010
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BBa_B0012
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BBa_J44000
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BBa_I715049
type: ComponentDefinition
persistent identity: BBa I715049
display id: BBa_I715049
version: 1
was generated by: iGEM to SBOL conversion
description: HPP-A1 + Hin
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:07:50Z
mutable provenance: Ligation of existing part
owned by: james
owned by: myers
mutable description: Part is composed of a Hin recombinase expression casette driven by pLac and series of flippable units that include halves of GFP and RFP: T7prom-RBS-RFP1-hixC-RFP2-RBS-GFP1-hixC-RFP2-TT-hixC-GFP2-TT-hixC . The corresponding part without Hin glows redas a result of the expression of the hix-interrupted RFP. With Hin, most colonies are red, but green colonies appear. Evidence suggests that this is a result of Hin-mediated recombination.
discontinued: false
dominant: true
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1858
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None
star: false
creator: Amanda Valencia
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_R0010
component: BBa_J31001
component: BBa_B0030
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_R0085
component: BBa_J44000
component: BBa_I715022
component: BBa_I715023
component: BBa_I715019
component: BBa_I715020
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_J31001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0085
sequence annotation: BBa_B0034
sequence annotation: BBa_I715022
sequence annotation: BBa_J44000
sequence annotation: BBa_I715023
sequence annotation: BBa_I715019
sequence annotation: BBa_I715020
sequence: https://synbiohub.org/public/igem/BBa_I715049_sequence/1

BBa_R0010
type: Component
persistent identity: component1954160
display id: component1954160
version: 1
top level: BBa_I715049
definition: LacI
access: public

BBa_J31001
type: Component
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display id: component1954175
version: 1
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definition: Hin LVA
access: public

BBa_B0030
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display id: component1954168
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definition: BBa_B0030
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BBa_B0012
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BBa_B0010
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BBa_B0034
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BBa_R0085
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BBa_J44000
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definition: hixC
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BBa_I715022
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definition: RFP1
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BBa_I715023
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BBa_J44000
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definition: hixC
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BBa_I715019
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definition: GFP1
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definition: RFP2
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definition: GFP2
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BBa_R0010
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location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954175/range1954175/1
component: BBa_J31001

https://synbiohub.org/public/igem/BBa_I715049/annotation1954175/range1954175/1
type: Range
persistent identity: range1954175
display id: range1954175
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 230
end: 841
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1954176
display id: annotation1954176
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954176/range1954176/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715049/annotation1954176/range1954176/1
type: Range
persistent identity: range1954176
display id: range1954176
version: 1
top level: BBa_I715049
direction: direction/forward
start: 850
end: 929
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1954178
display id: annotation1954178
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954178/range1954178/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715049/annotation1954178/range1954178/1
type: Range
persistent identity: range1954178
display id: range1954178
version: 1
top level: BBa_I715049
direction: direction/forward
start: 938
end: 978
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1954185
display id: annotation1954185
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954185/range1954185/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_I715049/annotation1954185/range1954185/1
type: Range
persistent identity: range1954185
display id: range1954185
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 987
end: 1009
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1954187
display id: annotation1954187
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954187/range1954187/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715049/annotation1954187/range1954187/1
type: Range
persistent identity: range1954187
display id: range1954187
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 1018
end: 1029
orientation: inline

BBa_I715022
type: SequenceAnnotation
persistent identity: annotation1954190
display id: annotation1954190
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954190/range1954190/1
component: BBa_I715022

https://synbiohub.org/public/igem/BBa_I715049/annotation1954190/range1954190/1
type: Range
persistent identity: range1954190
display id: range1954190
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 1036
end: 1497
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954191
display id: annotation1954191
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954191/range1954191/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715049/annotation1954191/range1954191/1
type: Range
persistent identity: range1954191
display id: range1954191
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 1506
end: 1531
orientation: inline

BBa_I715023
type: SequenceAnnotation
persistent identity: annotation1954194
display id: annotation1954194
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954194/range1954194/1
component: BBa_I715023

https://synbiohub.org/public/igem/BBa_I715049/annotation1954194/range1954194/1
type: Range
persistent identity: range1954194
display id: range1954194
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 1540
end: 1759
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1954196
display id: annotation1954196
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954196/range1954196/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715049/annotation1954196/range1954196/1
type: Range
persistent identity: range1954196
display id: range1954196
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 1768
end: 1779
orientation: inline

BBa_I715019
type: SequenceAnnotation
persistent identity: annotation1954199
display id: annotation1954199
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954199/range1954199/1
component: BBa_I715019

https://synbiohub.org/public/igem/BBa_I715049/annotation1954199/range1954199/1
type: Range
persistent identity: range1954199
display id: range1954199
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 1786
end: 2256
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954200
display id: annotation1954200
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954200/range1954200/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715049/annotation1954200/range1954200/1
type: Range
persistent identity: range1954200
display id: range1954200
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 2265
end: 2290
orientation: inline

BBa_I715023
type: SequenceAnnotation
persistent identity: annotation1954203
display id: annotation1954203
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954203/range1954203/1
component: BBa_I715023

https://synbiohub.org/public/igem/BBa_I715049/annotation1954203/range1954203/1
type: Range
persistent identity: range1954203
display id: range1954203
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 2299
end: 2518
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1954204
display id: annotation1954204
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954204/range1954204/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715049/annotation1954204/range1954204/1
type: Range
persistent identity: range1954204
display id: range1954204
version: 1
top level: BBa_I715049
direction: direction/forward
start: 2527
end: 2606
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1954206
display id: annotation1954206
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954206/range1954206/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715049/annotation1954206/range1954206/1
type: Range
persistent identity: range1954206
display id: range1954206
version: 1
top level: BBa_I715049
direction: direction/forward
start: 2615
end: 2655
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954210
display id: annotation1954210
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954210/range1954210/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715049/annotation1954210/range1954210/1
type: Range
persistent identity: range1954210
display id: range1954210
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 2664
end: 2689
orientation: inline

BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1954213
display id: annotation1954213
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954213/range1954213/1
component: BBa_I715020

https://synbiohub.org/public/igem/BBa_I715049/annotation1954213/range1954213/1
type: Range
persistent identity: range1954213
display id: range1954213
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 2698
end: 2947
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1954214
display id: annotation1954214
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954214/range1954214/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715049/annotation1954214/range1954214/1
type: Range
persistent identity: range1954214
display id: range1954214
version: 1
top level: BBa_I715049
direction: direction/forward
start: 2956
end: 3035
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1954216
display id: annotation1954216
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954216/range1954216/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715049/annotation1954216/range1954216/1
type: Range
persistent identity: range1954216
display id: range1954216
version: 1
top level: BBa_I715049
direction: direction/forward
start: 3044
end: 3084
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1954220
display id: annotation1954220
version: 1
top level: BBa_I715049
location: https://synbiohub.org/public/igem/BBa_I715049/annotation1954220/range1954220/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715049/annotation1954220/range1954220/1
type: Range
persistent identity: range1954220
display id: range1954220
version: 1
top level: BBa_I715049
direction: direction/reverse
start: 3093
end: 3118
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

RFP1
type: ComponentDefinition
persistent identity: BBa I715022
display id: BBa_I715022
version: 1
was generated by: iGEM to SBOL conversion
description: Amino Portion of RFP
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:43:58Z
mutable provenance: adf
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: af
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
sample status: In stock
status: status/Available
bookmark: false
mutable notes: afd
star: true
creator: Andrew Martens
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I715022_sequence/1

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1935381
display id: annotation1935381
version: 1
top level: RFP1
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
type: Range
persistent identity: range1935381
display id: range1935381
version: 1
top level: RFP1
direction: direction/forward
start: 1
end: 462
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1935380
display id: annotation1935380
version: 1
top level: RFP1
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
type: Range
persistent identity: range1935380
display id: range1935380
version: 1
top level: RFP1
direction: direction/forward
start: 1
end: 3
orientation: inline

RFP2
type: ComponentDefinition
persistent identity: BBa I715023
display id: BBa_I715023
version: 1
was generated by: iGEM to SBOL conversion
description: Carboxyl portion of RFP
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:44:49Z
mutable provenance: Sequence from part BBa_E1010.
owned by: james
owned by: myers
mutable description: The second portion of the RFP gene, after the HixC insertion point.
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Used web tool.
star: true
creator: Andrew Martens
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: extra base (avoid frameshift)
sequence annotation: Continue coding sequence
sequence annotation: (two in tandem)
sequence: https://synbiohub.org/public/igem/BBa_I715023_sequence/1

extra base (avoid frameshift)
type: SequenceAnnotation
persistent identity: annotation1935382
display id: annotation1935382
version: 1
top level: RFP2
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1
type: Range
persistent identity: range1935382
display id: range1935382
version: 1
top level: RFP2
direction: direction/forward
start: 1
end: 1
orientation: inline

Continue coding sequence
type: SequenceAnnotation
persistent identity: annotation1935383
display id: annotation1935383
version: 1
top level: RFP2
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1
type: Range
persistent identity: range1935383
display id: range1935383
version: 1
top level: RFP2
direction: direction/forward
start: 2
end: 214
orientation: inline

(two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935384
display id: annotation1935384
version: 1
top level: RFP2
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1
type: Range
persistent identity: range1935384
display id: range1935384
version: 1
top level: RFP2
direction: direction/forward
start: 215
end: 219
orientation: inline

Hin LVA
type: ComponentDefinition
persistent identity: BBa J31001
display id: BBa_J31001
version: 1
was generated by: iGEM to SBOL conversion
description: DNA invertase Hin tagged with LVA
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: Salmonella typhimurium and the the Hin part without LVA.
owned by: james
owned by: myers
mutable description: This part produces the Hin protein with an LVA degredation tag. We are currently looking into this as a way to ensure no Hin is floating around once we turn off the Hin promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None really
star: false
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Fwd primer J31001
sequence annotation: Hin
sequence annotation: Rev primer J31001
sequence annotation: LVA
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_J31001_sequence/1

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910565
display id: annotation1910565
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1
type: Range
persistent identity: range1910565
display id: range1910565
version: 1
top level: Hin LVA
direction: direction/forward
start: 1
end: 3
orientation: inline

Hin
type: SequenceAnnotation
persistent identity: annotation1910564
display id: annotation1910564
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1
type: Range
persistent identity: range1910564
display id: range1910564
version: 1
top level: Hin LVA
direction: direction/forward
start: 1
end: 570
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910570
display id: annotation1910570
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1
type: Range
persistent identity: range1910570
display id: range1910570
version: 1
top level: Hin LVA
direction: direction/reverse
start: 551
end: 612
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1910566
display id: annotation1910566
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1
type: Range
persistent identity: range1910566
display id: range1910566
version: 1
top level: Hin LVA
direction: direction/forward
start: 571
end: 609
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation1910567
display id: annotation1910567
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1
type: Range
persistent identity: range1910567
display id: range1910567
version: 1
top level: Hin LVA
direction: direction/forward
start: 610
end: 612
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: iGEM to SBOL conversion
description: hixC binding site for Salmonella typhimurium Hin recombinase
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
star: true
creator: Missouri Western and Davidson Groups, Todd Eckdahl
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

GFP1
type: ComponentDefinition
persistent identity: BBa I715019
display id: BBa_I715019
version: 1
was generated by: iGEM to SBOL conversion
description: Amino Half of GFP (aka GFP1)
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
mutable provenance: A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: A
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: A
star: true
creator: Will DeLoache
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Coding Amino Portion GFP (aka GFP1)
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_I715019_sequence/1

Coding Amino Portion GFP (aka GFP1)
type: SequenceAnnotation
persistent identity: annotation1935357
display id: annotation1935357
version: 1
top level: GFP1
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1
type: Range
persistent identity: range1935357
display id: range1935357
version: 1
top level: GFP1
direction: direction/forward
start: 1
end: 471
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1935356
display id: annotation1935356
version: 1
top level: GFP1
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1
type: Range
persistent identity: range1935356
display id: range1935356
version: 1
top level: GFP1
direction: direction/forward
start: 1
end: 3
orientation: inline

GFP2
type: ComponentDefinition
persistent identity: BBa I715020
display id: BBa_I715020
version: 1
was generated by: iGEM to SBOL conversion
description: Carboxyl Half of GFP (aka GFP2)
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
mutable provenance: A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: A
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: A
star: true
creator: Will DeLoache
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Coding Carboxyl half of GFP (aka GFP2)
sequence annotation: extra base to maintain reading frame
sequence annotation: Stop Codons (two in tandem)
sequence: https://synbiohub.org/public/igem/BBa_I715020_sequence/1

Coding Carboxyl half of GFP (aka GFP2)
type: SequenceAnnotation
persistent identity: annotation1935361
display id: annotation1935361
version: 1
top level: GFP2
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1
type: Range
persistent identity: range1935361
display id: range1935361
version: 1
top level: GFP2
direction: direction/forward
start: 1
end: 244
orientation: inline

extra base to maintain reading frame
type: SequenceAnnotation
persistent identity: annotation1935360
display id: annotation1935360
version: 1
top level: GFP2
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1
type: Range
persistent identity: range1935360
display id: range1935360
version: 1
top level: GFP2
direction: direction/forward
start: 1
end: 1
orientation: inline

Stop Codons (two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935362
display id: annotation1935362
version: 1
top level: GFP2
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1
type: Range
persistent identity: range1935362
display id: range1935362
version: 1
top level: GFP2
direction: direction/forward
start: 245
end: 250
orientation: inline

https://synbiohub.org/public/igem/BBa_I715050_sequence/1
type: Sequence
persistent identity: BBa I715050 sequence
display id: BBa_I715050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagactactagagttatcaaaaaccatggtttttgataatactagagtggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715049_sequence/1
type: Sequence
persistent identity: BBa I715049 sequence
display id: BBa_I715049_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagactactagagttatcaaaaaccatggtttttgataatactagagtggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715022_sequence/1
type: Sequence
persistent identity: BBa I715022 sequence
display id: BBa_I715022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715023_sequence/1
type: Sequence
persistent identity: BBa I715023 sequence
display id: BBa_I715023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31001_sequence/1
type: Sequence
persistent identity: BBa J31001 sequence
display id: BBa_J31001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44000_sequence/1
type: Sequence
persistent identity: BBa J44000 sequence
display id: BBa_J44000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715019_sequence/1
type: Sequence
persistent identity: BBa I715019 sequence
display id: BBa_I715019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715020_sequence/1
type: Sequence
persistent identity: BBa I715020 sequence
display id: BBa_I715020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z