##gff-version 3
##sequence-region BBa_I715054 1 1264
BBa_I715054	.	terminator	1136	1215	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I715054	.	stem_loop	1147	1190	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I715054	.	engineered_region	1136	1215	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I715054	.	terminator	1224	1264	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I715054	.	polyA_site	1251	1264	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I715054	.	stop_codon	1257	1257	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I715054	.	engineered_region	1224	1264	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I715054	.	stem_loop	1231	1250	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I715054	.	engineered_region	369	1127	.	+	0	ID=BBa_J04031;Name=BBa_J04031
BBa_I715054	.	start_codon	369	371	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_I715054	.	tag	1083	1121	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_I715054	.	engineered_region	369	1088	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_I715054	.	stop_codon	1122	1127	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_I715054	.	ribosome_entry_site	351	362	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I715054	.	conserved_region	355	358	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715054	.	promoter	209	342	.	+	0	ID=BBa_I715052;Name=BBa_I715052
BBa_I715054	.	ribosome_entry_site	213	221	.	+	0	ID=annotation1958519;Name=Predicted RBS;Parent=BBa_I715052
BBa_I715054	.	start_codon	228	230	.	+	0	ID=annotation1958520;Name=start;Parent=BBa_I715052
BBa_I715054	.	CDS	228	272	.	+	0	ID=annotation1958523;Name=leader peptide;Parent=BBa_I715052
BBa_I715054	.	conserved_region	255	260	.	+	0	ID=annotation1958521;Name=tandem trp codons;Parent=BBa_I715052
BBa_I715054	.	stop_codon	270	272	.	+	0	ID=annotation1958522;Name=stop;Parent=BBa_I715052
BBa_I715054	.	sequence_feature	335	342	.	+	0	ID=annotation1958524;Name=Poly T;Parent=BBa_I715052
BBa_I715054	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I715054	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I715054	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I715054	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I715054	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I715054	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I715054	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I715054	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
>BBa_I715054
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagcgtaaaaagggtatcgacaatgaaagcaa
ttttcgtactgaaaggttggtggcgcacttcctgaaacgggcagtgtattcaccatgcgtaaagcaatcagatacccag
cccgcctaatgagcgggctttttttttactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcact
ggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtg
atgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcac
tactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatg
cccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttg
aaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaatt
ggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatt
agacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcc
ttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcct
tcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaac
tacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata