BBa_I715055
type: ComponentDefinition
persistent identity: BBa I715055
display id: BBa_I715055
version: 1
was generated by: iGEM to SBOL conversion
description: Test Trp anti-terminator/terminator with hixC insertion
created: 2007-11-07T12:00:00Z
modified: 2015-08-31T04:07:50Z
mutable provenance: More to come later
owned by: james
owned by: myers
mutable description: More to come later
discontinued: false
dominant: false
group u list: _120_
m user id: 0
owner id: 1620
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: More to come later
star: false
creator: Andrew Martens
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_R0010
component: BBa_S03903
component: BBa_I715053
sequence annotation: BBa_R0010
sequence annotation: BBa_I715053
sequence annotation: BBa_S03903
sequence: https://synbiohub.org/public/igem/BBa_I715055_sequence/1

BBa_R0010
type: Component
persistent identity: component2223961
display id: component2223961
version: 1
top level: BBa_I715055
definition: LacI
access: public

BBa_S03903
type: Component
persistent identity: component2223989
display id: component2223989
version: 1
top level: BBa_I715055
definition: BBa_S03903
access: public

BBa_I715053
type: Component
persistent identity: component2223975
display id: component2223975
version: 1
top level: BBa_I715055
definition: BBa_I715053
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2223961
display id: annotation2223961
version: 1
top level: BBa_I715055
location: https://synbiohub.org/public/igem/BBa_I715055/annotation2223961/range2223961/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I715055/annotation2223961/range2223961/1
type: Range
persistent identity: range2223961
display id: range2223961
version: 1
top level: BBa_I715055
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_I715053
type: SequenceAnnotation
persistent identity: annotation2223975
display id: annotation2223975
version: 1
top level: BBa_I715055
location: https://synbiohub.org/public/igem/BBa_I715055/annotation2223975/range2223975/1
component: BBa_I715053

https://synbiohub.org/public/igem/BBa_I715055/annotation2223975/range2223975/1
type: Range
persistent identity: range2223975
display id: range2223975
version: 1
top level: BBa_I715055
direction: direction/reverse
start: 209
end: 367
orientation: inline

BBa_S03903
type: SequenceAnnotation
persistent identity: annotation2223989
display id: annotation2223989
version: 1
top level: BBa_I715055
location: https://synbiohub.org/public/igem/BBa_I715055/annotation2223989/range2223989/1
component: BBa_S03903

https://synbiohub.org/public/igem/BBa_I715055/annotation2223989/range2223989/1
type: Range
persistent identity: range2223989
display id: range2223989
version: 1
top level: BBa_I715055
direction: direction/reverse
start: 376
end: 1289
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_S03903
type: ComponentDefinition
persistent identity: BBa S03903
display id: BBa_S03903
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:J04631
created: 2007-11-07T12:00:00Z
modified: 2015-05-08T01:14:32Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
experience: experience/None
group u list: _9_
m user id: 0
owner id: 1620
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Andrew Martens
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_J04031
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_J04031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_S03903_sequence/1

BBa_B0034
type: Component
persistent identity: component1958507
display id: component1958507
version: 1
top level: BBa_S03903
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1958513
display id: component1958513
version: 1
top level: BBa_S03903
definition: BBa_B0010
access: public

BBa_J04031
type: Component
persistent identity: component1958512
display id: component1958512
version: 1
top level: BBa_S03903
definition: BBa_J04031
access: public

BBa_B0012
type: Component
persistent identity: component1958515
display id: component1958515
version: 1
top level: BBa_S03903
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1958507
display id: annotation1958507
version: 1
top level: BBa_S03903
location: https://synbiohub.org/public/igem/BBa_S03903/annotation1958507/range1958507/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S03903/annotation1958507/range1958507/1
type: Range
persistent identity: range1958507
display id: range1958507
version: 1
top level: BBa_S03903
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation1958512
display id: annotation1958512
version: 1
top level: BBa_S03903
location: https://synbiohub.org/public/igem/BBa_S03903/annotation1958512/range1958512/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_S03903/annotation1958512/range1958512/1
type: Range
persistent identity: range1958512
display id: range1958512
version: 1
top level: BBa_S03903
direction: direction/reverse
start: 19
end: 777
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1958513
display id: annotation1958513
version: 1
top level: BBa_S03903
location: https://synbiohub.org/public/igem/BBa_S03903/annotation1958513/range1958513/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S03903/annotation1958513/range1958513/1
type: Range
persistent identity: range1958513
display id: range1958513
version: 1
top level: BBa_S03903
direction: direction/forward
start: 786
end: 865
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1958515
display id: annotation1958515
version: 1
top level: BBa_S03903
location: https://synbiohub.org/public/igem/BBa_S03903/annotation1958515/range1958515/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S03903/annotation1958515/range1958515/1
type: Range
persistent identity: range1958515
display id: range1958515
version: 1
top level: BBa_S03903
direction: direction/forward
start: 874
end: 914
orientation: inline

BBa_I715053
type: ComponentDefinition
persistent identity: BBa I715053
display id: BBa_I715053
version: 1
was generated by: iGEM to SBOL conversion
description: Trp Leader Peptide and anti-terminator/terminator with hixC insertion
created: 2007-11-07T12:00:00Z
modified: 2015-08-31T04:07:50Z
mutable provenance: ibid
owned by: james
owned by: myers
mutable description: More info later
discontinued: false
dominant: false
group u list: _120_
m user id: 0
owner id: 1620
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ibid
star: false
creator: Andrew Martens
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: predicted RBS
sequence annotation: leader peptide
sequence annotation: start
sequence annotation: tandem trp codons
sequence annotation: stop
sequence annotation: hixC
sequence annotation: Poly T
sequence: https://synbiohub.org/public/igem/BBa_I715053_sequence/1

predicted RBS
type: SequenceAnnotation
persistent identity: annotation1958526
display id: annotation1958526
version: 1
top level: BBa_I715053
location: https://synbiohub.org/public/igem/BBa_I715053/annotation1958526/range1958526/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I715053/annotation1958526/range1958526/1
type: Range
persistent identity: range1958526
display id: range1958526
version: 1
top level: BBa_I715053
direction: direction/forward
start: 5
end: 13
orientation: inline

leader peptide
type: SequenceAnnotation
persistent identity: annotation1958527
display id: annotation1958527
version: 1
top level: BBa_I715053
location: https://synbiohub.org/public/igem/BBa_I715053/annotation1958527/range1958527/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715053/annotation1958527/range1958527/1
type: Range
persistent identity: range1958527
display id: range1958527
version: 1
top level: BBa_I715053
direction: direction/forward
start: 20
end: 64
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1958525
display id: annotation1958525
version: 1
top level: BBa_I715053
location: https://synbiohub.org/public/igem/BBa_I715053/annotation1958525/range1958525/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I715053/annotation1958525/range1958525/1
type: Range
persistent identity: range1958525
display id: range1958525
version: 1
top level: BBa_I715053
direction: direction/forward
start: 20
end: 22
orientation: inline

tandem trp codons
type: SequenceAnnotation
persistent identity: annotation1958528
display id: annotation1958528
version: 1
top level: BBa_I715053
location: https://synbiohub.org/public/igem/BBa_I715053/annotation1958528/range1958528/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_I715053/annotation1958528/range1958528/1
type: Range
persistent identity: range1958528
display id: range1958528
version: 1
top level: BBa_I715053
direction: direction/forward
start: 47
end: 52
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1958529
display id: annotation1958529
version: 1
top level: BBa_I715053
location: https://synbiohub.org/public/igem/BBa_I715053/annotation1958529/range1958529/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I715053/annotation1958529/range1958529/1
type: Range
persistent identity: range1958529
display id: range1958529
version: 1
top level: BBa_I715053
direction: direction/forward
start: 62
end: 64
orientation: inline

hixC
type: SequenceAnnotation
persistent identity: annotation1958531
display id: annotation1958531
version: 1
top level: BBa_I715053
location: https://synbiohub.org/public/igem/BBa_I715053/annotation1958531/range1958531/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_I715053/annotation1958531/range1958531/1
type: Range
persistent identity: range1958531
display id: range1958531
version: 1
top level: BBa_I715053
direction: direction/forward
start: 91
end: 116
orientation: inline

Poly T
type: SequenceAnnotation
persistent identity: annotation1958530
display id: annotation1958530
version: 1
top level: BBa_I715053
location: https://synbiohub.org/public/igem/BBa_I715053/annotation1958530/range1958530/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I715053/annotation1958530/range1958530/1
type: Range
persistent identity: range1958530
display id: range1958530
version: 1
top level: BBa_I715053
direction: direction/forward
start: 152
end: 159
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J04031
type: ComponentDefinition
persistent identity: BBa J04031
display id: BBa_J04031
version: 1
was generated by: iGEM to SBOL conversion
description: GFP w/LVA tag
created: 2005-06-07T11:00:00Z
modified: 2015-08-31T04:08:13Z
mutable provenance: Davidson Synth Aces
owned by: james
owned by: myers
mutable description: BBa_E0040 with an LVA tag added via PCR. BBa_E0040 is GFP(mut3b).
discontinued: false
dominant: true
experience: experience/None
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Start
sequence annotation: BBa_E0040
sequence annotation: LVA
sequence annotation: Double Stop
sequence: https://synbiohub.org/public/igem/BBa_J04031_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation1507246
display id: annotation1507246
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507246/range1507246/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J04031/annotation1507246/range1507246/1
type: Range
persistent identity: range1507246
display id: range1507246
version: 1
top level: BBa_J04031
direction: direction/forward
start: 1
end: 3
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1507202
display id: annotation1507202
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507202/range1507202/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_J04031/annotation1507202/range1507202/1
type: Range
persistent identity: range1507202
display id: range1507202
version: 1
top level: BBa_J04031
direction: direction/forward
start: 1
end: 720
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1507235
display id: annotation1507235
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507235/range1507235/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_J04031/annotation1507235/range1507235/1
type: Range
persistent identity: range1507235
display id: range1507235
version: 1
top level: BBa_J04031
direction: direction/forward
start: 715
end: 753
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation1507242
display id: annotation1507242
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507242/range1507242/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J04031/annotation1507242/range1507242/1
type: Range
persistent identity: range1507242
display id: range1507242
version: 1
top level: BBa_J04031
direction: direction/forward
start: 754
end: 759
orientation: inline

https://synbiohub.org/public/igem/BBa_I715055_sequence/1
type: Sequence
persistent identity: BBa I715055 sequence
display id: BBa_I715055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagcgtaaaaagggtatcgacaatgaaagcaattttcgtactgaaaggttggtggcgcacttcctgaaacgggcagtgtattcaccatgcgtattatcaaaaaccatggtttttgataaagcaatcagatacccagcccgcctaatgagcgggctttttttttactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S03903_sequence/1
type: Sequence
persistent identity: BBa S03903 sequence
display id: BBa_S03903_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715053_sequence/1
type: Sequence
persistent identity: BBa I715053 sequence
display id: BBa_I715053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaaagggtatcgacaatgaaagcaattttcgtactgaaaggttggtggcgcacttcctgaaacgggcagtgtattcaccatgcgtattatcaaaaaccatggtttttgataaagcaatcagatacccagcccgcctaatgagcgggctttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04031_sequence/1
type: Sequence
persistent identity: BBa J04031 sequence
display id: BBa_J04031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z