LOCUS       BBa_I715056              812 bp    DNA     linear       22-Nov-2024
DEFINITION  T7-RBS-GFP1-hixC-GFP2
ACCESSION   BBa_I715056
VERSION     BBa_I715056.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=Start Codon
     CDS             50..520
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    50..520
                     /label=BBa_I715019
     misc_feature    529..554
                     /label=BBa_J44000
     misc_feature    563..563
                     /label=extra base to maintain reading frame
     CDS             563..806
                     /label=Coding Carboxyl half of GFP (aka GFP2)
     misc_feature    807..812
                     /label=Stop Codons (two in tandem)
     misc_feature    563..812
                     /label=BBa_I715020
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgcgtaaagg
       61 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      121 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      181 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      241 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      301 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      361 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      421 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      481 ctataactca cacaatgtat acatcatggc agacaaacaa tactagagtt atcaaaaacc
      541 atggtttttg ataatactag agtaagaatg gaatcaaagt taacttcaaa attagacaca
      601 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
      661 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
      721 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
      781 cacatggcat ggatgaacta tacaaataat aa
//