LOCUS       BBa_I715057              782 bp    DNA     linear       22-Dec-2024
DEFINITION  T7-RBS-GFP1-hixC-RFP2
ACCESSION   BBa_I715057
VERSION     BBa_I715057.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     misc_feature    50..52
                     /label=Start Codon
     CDS             50..520
                     /label=Coding Amino Portion GFP (aka GFP1)
     misc_feature    50..520
                     /label=BBa_I715019
     misc_feature    529..554
                     /label=BBa_J44000
     misc_feature    563..563
                     /label=extra base (avoid frameshift)
     CDS             564..776
                     /label=Continue coding sequence
     misc_feature    777..781
                     /label=(two in tandem)
     CDS             563..782
                     /label=BBa_I715023
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tgcgtaaagg
       61 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      121 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      181 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      241 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt
      301 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
      361 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      421 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa
      481 ctataactca cacaatgtat acatcatggc agacaaacaa tactagagtt atcaaaaacc
      541 atggtttttg ataatactag agtggtgctc tgaaaggtga aatcaaaatg cgtctgaaac
      601 tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg
      661 ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag
      721 actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat
      781 aa
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