LOCUS BBa_I715058 3499 bp DNA linear 22-Dec-2024 DEFINITION HPP - B1 (DBA) + Hin ACCESSION BBa_I715058 VERSION BBa_I715058.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..799 /label=Hin primer_bind 230..232 /label=Fwd primer J31001 misc_feature 800..838 /label=LVA misc_feature 839..841 /label=Stop codon primer_bind 780..841 /label=Rev primer J31001 CDS 230..841 /label=BBa_J31001 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 misc_feature 1004..1004 /label=Transcription Start Site misc_feature 987..997 /label=Polymerase Binding Region misc_feature 998..1009 /label=Initiation Region promoter 987..1009 /label=BBa_R0085 misc_feature 1022..1025 /label=conserved RBS 1018..1029 /label=BBa_B0034 misc_feature 1036..1038 /label=start CDS 1036..1497 /label=Coding sequence CDS 1036..1497 /label=BBa_I715022 misc_feature 1506..1531 /label=BBa_J44000 misc_feature 1540..1540 /label=extra base to maintain reading frame CDS 1540..1783 /label=Coding Carboxyl half of GFP (aka GFP2) misc_feature 1784..1789 /label=Stop Codons (two in tandem) misc_feature 1540..1789 /label=BBa_I715020 misc_feature 1802..1805 /label=conserved RBS 1798..1809 /label=BBa_B0034 misc_feature 1816..1818 /label=start CDS 1816..2277 /label=Coding sequence CDS 1816..2277 /label=BBa_I715022 misc_feature 2286..2311 /label=BBa_J44000 misc_feature 2320..2320 /label=extra base to maintain reading frame CDS 2320..2563 /label=Coding Carboxyl half of GFP (aka GFP2) misc_feature 2564..2569 /label=Stop Codons (two in tandem) misc_feature 2320..2569 /label=BBa_I715020 stem_loop 2589..2632 /label=stem_loop misc_feature 2578..2657 /label=BBa_B0010 terminator 2578..2657 /label=BBa_B0010 stem_loop 2673..2692 /label=T7 TE misc_feature 2699..2699 /label=stop polyA_site 2693..2706 /label=polya misc_feature 2666..2706 /label=BBa_B0012 terminator 2666..2706 /label=BBa_B0012 misc_feature 2715..2740 /label=BBa_J44000 misc_feature 2749..2749 /label=extra base (avoid frameshift) CDS 2750..2962 /label=Continue coding sequence misc_feature 2963..2967 /label=(two in tandem) CDS 2749..2968 /label=BBa_I715023 misc_feature 2981..2984 /label=conserved RBS 2977..2988 /label=BBa_B0034 misc_feature 2995..2997 /label=Start Codon CDS 2995..3465 /label=Coding Amino Portion GFP (aka GFP1) misc_feature 2995..3465 /label=BBa_I715019 misc_feature 3474..3499 /label=BBa_J44000 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggctactat 241 tgggtatatt cgggtgtcaa caattgacca aaatatcgat ttacagcgta atgcgcttac 301 cagtgcaaat tgtgaccgca tttttgagga ccgtatcagt ggcaagattg caaaccgccc 361 cggcctgaaa cgagcgttaa agtatgtaaa taaaggcgat actcttgtcg tctggaaatt 421 agacagactg ggccgcagcg tgaaaaacct ggtggcgtta atatcagaat tacatgaacg 481 tggagctcac ttccattctt taaccgatag tattgatacc agtagcgcga tggggcgatt 541 cttttttcat gtaatgtcag cactggccga gatggagcga gaattaattg tcgagcgaac 601 ccttgccgga ctggctgccg ccagagcgca aggacgactg ggagggcgcc ctcgggcgat 661 caacagacat gaacaggaac agattagtcg gctattagag aaaggccatc ctcggcagca 721 actagctatt atttttggta ttggcgtatc taccttatac agatattttc cggcaagccg 781 tataaaaaaa cgaatgaata ggcctgctgc aaacgacgaa aactacgctt tagtagctta 841 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagagtaat acgactcact atagggagat actagagaaa 1021 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa 1081 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt 1141 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg 1201 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac 1261 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt 1321 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac 1381 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt 1441 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagactac 1501 tagagttatc aaaaaccatg gtttttgata atactagagt aagaatggaa tcaaagttaa 1561 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 1621 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 1681 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 1741 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagaaa 1801 gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa 1861 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt 1921 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg 1981 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac 2041 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt 2101 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac 2161 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt 2221 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagactac 2281 tagagttatc aaaaaccatg gtttttgata atactagagt aagaatggaa tcaaagttaa 2341 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 2401 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 2461 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 2521 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca 2581 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2641 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2701 tttatatact agagttatca aaaaccatgg tttttgataa tactagagtg gtgctctgaa 2761 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa 2821 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa 2881 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga 2941 aggtcgtcac tccaccggtg cttaataata ctagagaaag aggagaaata ctagatgcgt 3001 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 3061 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt 3121 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 3181 actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac 3241 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat 3301 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga 3361 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa 3421 tacaactata actcacacaa tgtatacatc atggcagaca aacaatacta gagttatcaa 3481 aaaccatggt ttttgataa //