##gff-version 3
##sequence-region BBa_I715058 1 3499
BBa_I715058	.	promoter	987	1009	.	+	0	ID=BBa_R0085;Name=BBa_R0085
BBa_I715058	.	sequence_feature	998	1009	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
BBa_I715058	.	non_covalent_binding_site	987	997	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_I715058	.	sequence_feature	1004	1004	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
BBa_I715058	.	engineered_region	2320	2569	.	+	0	ID=BBa_I715020;Name=BBa_I715020
BBa_I715058	.	sequence_feature	2320	2320	.	+	0	ID=annotation1935360;Name=extra base to maintain reading frame;Parent=BBa_I715020
BBa_I715058	.	CDS	2320	2563	.	+	0	ID=annotation1935361;Name=Coding Carboxyl half of GFP (aka GFP2);Parent=BBa_I715020
BBa_I715058	.	stop_codon	2564	2569	.	+	0	ID=annotation1935362;Name=Stop Codons (two in tandem);Parent=BBa_I715020
BBa_I715058	.	engineered_region	2995	3465	.	+	0	ID=BBa_I715019;Name=BBa_I715019
BBa_I715058	.	CDS	2995	3465	.	+	0	ID=annotation1935357;Name=Coding Amino Portion GFP (aka GFP1);Parent=BBa_I715019
BBa_I715058	.	start_codon	2995	2997	.	+	0	ID=annotation1935356;Name=Start Codon;Parent=BBa_I715019
BBa_I715058	.	ribosome_entry_site	1018	1029	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I715058	.	conserved_region	1022	1025	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715058	.	CDS	1816	2277	.	+	0	ID=BBa_I715022;Name=BBa_I715022
BBa_I715058	.	CDS	1816	2277	.	+	0	ID=annotation1935381;Name=Coding sequence;Parent=BBa_I715022
BBa_I715058	.	start_codon	1816	1818	.	+	0	ID=annotation1935380;Name=start;Parent=BBa_I715022
BBa_I715058	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I715058	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I715058	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I715058	.	ribosome_entry_site	1798	1809	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I715058	.	conserved_region	1802	1805	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715058	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I715058	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I715058	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I715058	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I715058	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I715058	.	CDS	2749	2968	.	+	0	ID=BBa_I715023;Name=BBa_I715023
BBa_I715058	.	CDS	2750	2962	.	+	0	ID=annotation1935383;Name=Continue coding sequence;Parent=BBa_I715023
BBa_I715058	.	stop_codon	2963	2967	.	+	0	ID=annotation1935384;Name=(two in tandem);Parent=BBa_I715023
BBa_I715058	.	sequence_feature	2749	2749	.	+	0	ID=annotation1935382;Name=extra base (avoid frameshift);Parent=BBa_I715023
BBa_I715058	.	CDS	1036	1497	.	+	0	ID=BBa_I715022;Name=BBa_I715022
BBa_I715058	.	CDS	1036	1497	.	+	0	ID=annotation1935381;Name=Coding sequence;Parent=BBa_I715022
BBa_I715058	.	start_codon	1036	1038	.	+	0	ID=annotation1935380;Name=start;Parent=BBa_I715022
BBa_I715058	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I715058	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I715058	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I715058	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I715058	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I715058	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I715058	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I715058	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I715058	.	terminator	2666	2706	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I715058	.	polyA_site	2693	2706	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I715058	.	stem_loop	2673	2692	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I715058	.	stop_codon	2699	2699	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I715058	.	engineered_region	2666	2706	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I715058	.	sequence_feature	2715	2740	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715058	.	ribosome_entry_site	2977	2988	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I715058	.	conserved_region	2981	2984	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715058	.	CDS	230	841	.	+	0	ID=BBa_J31001;Name=BBa_J31001
BBa_I715058	.	tag	800	838	.	+	0	ID=annotation1910566;Name=LVA;Parent=BBa_J31001
BBa_I715058	.	primer_binding_site	780	841	.	+	0	ID=annotation1910570;Name=Rev primer J31001;Parent=BBa_J31001
BBa_I715058	.	stop_codon	839	841	.	+	0	ID=annotation1910567;Name=Stop codon;Parent=BBa_J31001
BBa_I715058	.	CDS	230	799	.	+	0	ID=annotation1910564;Name=Hin;Parent=BBa_J31001
BBa_I715058	.	primer_binding_site	230	232	.	+	0	ID=annotation1910565;Name=Fwd primer J31001;Parent=BBa_J31001
BBa_I715058	.	sequence_feature	1506	1531	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715058	.	sequence_feature	3474	3499	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715058	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I715058	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I715058	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I715058	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I715058	.	sequence_feature	2286	2311	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715058	.	engineered_region	1540	1789	.	+	0	ID=BBa_I715020;Name=BBa_I715020
BBa_I715058	.	sequence_feature	1540	1540	.	+	0	ID=annotation1935360;Name=extra base to maintain reading frame;Parent=BBa_I715020
BBa_I715058	.	CDS	1540	1783	.	+	0	ID=annotation1935361;Name=Coding Carboxyl half of GFP (aka GFP2);Parent=BBa_I715020
BBa_I715058	.	stop_codon	1784	1789	.	+	0	ID=annotation1935362;Name=Stop Codons (two in tandem);Parent=BBa_I715020
BBa_I715058	.	terminator	2578	2657	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I715058	.	stem_loop	2589	2632	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I715058	.	engineered_region	2578	2657	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I715058
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctac
tattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgac
cgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaag
gcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattaca
tgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgta
atgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaag
gacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcc
tcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaa
cgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagaaagaggaga
aatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacgg
tcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaa
ggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccgg
ctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtgg
tgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttc
ccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagactact
agagttatcaaaaaccatggtttttgataatactagagtaagaatggaatcaaagttaacttcaaaattagacacaaca
ttgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccaga
caaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagttt
gtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagat
ggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttc
gaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgc
tgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatccc
ggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttacc
gttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacg
gtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagactactagagttatca
aaaaccatggtttttgataatactagagtaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatgg
aagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattac
ctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctg
ctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagttatcaaaaaccatggtttttgataatactagagtggtgctctgaaaggtg
aaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaacc
ggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaa
cagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagagaaagaggagaaatactagatgcgtaa
aggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtc
agtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttc
catggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatga
ctttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagaca
cgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaa
acattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaatactagagtta
tcaaaaaccatggtttttgataa