BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_I715058
1
BBa_I715058
HPP - B1 (DBA) + Hin
2007-11-25T12:00:00Z
2015-08-31T04:07:50Z
See subparts.
This part has our DBA construct downtream of the Hin expression cassette.
false
false
_120_
0
1491
9
Not in stock
false
None.
false
Will DeLoache
component1958804
1
BBa_I715019
component1958789
1
BBa_I715020
component1958792
1
BBa_B0012
component1958799
1
BBa_I715023
component1958786
1
BBa_J44000
component1958771
1
BBa_R0085
component1958776
1
BBa_I715022
component1958746
1
BBa_R0010
component1958777
1
BBa_J44000
component1958773
1
BBa_B0034
component1958790
1
BBa_B0010
component1958785
1
BBa_I715022
component1958761
1
BBa_J31001
component1958782
1
BBa_B0034
component1958801
1
BBa_B0034
component1958754
1
BBa_B0030
component1958764
1
BBa_B0012
component1958762
1
BBa_B0010
component1958796
1
BBa_J44000
component1958805
1
BBa_J44000
component1958780
1
BBa_I715020
annotation1958805
1
BBa_J44000
range1958805
1
3474
3499
annotation1958792
1
BBa_B0012
range1958792
1
2666
2706
annotation1958796
1
BBa_J44000
range1958796
1
2715
2740
annotation1958790
1
BBa_B0010
range1958790
1
2578
2657
annotation1958782
1
BBa_B0034
range1958782
1
1798
1809
annotation1958780
1
BBa_I715020
range1958780
1
1540
1789
annotation1958773
1
BBa_B0034
range1958773
1
1018
1029
annotation1958764
1
BBa_B0012
range1958764
1
938
978
annotation1958799
1
BBa_I715023
range1958799
1
2749
2968
annotation1958801
1
BBa_B0034
range1958801
1
2977
2988
annotation1958761
1
BBa_J31001
range1958761
1
230
841
annotation1958746
1
BBa_R0010
range1958746
1
1
200
annotation1958776
1
BBa_I715022
range1958776
1
1036
1497
annotation1958771
1
BBa_R0085
range1958771
1
987
1009
annotation1958789
1
BBa_I715020
range1958789
1
2320
2569
annotation1958785
1
BBa_I715022
range1958785
1
1816
2277
annotation1958804
1
BBa_I715019
range1958804
1
2995
3465
annotation1958762
1
BBa_B0010
range1958762
1
850
929
annotation1958786
1
BBa_J44000
range1958786
1
2286
2311
annotation1958754
1
BBa_B0030
range1958754
1
209
223
annotation1958777
1
BBa_J44000
range1958777
1
1506
1531
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0085
1
BBa_R0085
T7 Consensus Promoter Sequence
2005-02-21T12:00:00Z
2015-05-08T01:14:15Z
Sequence obtained from Sri Kosuri
Released HQ 2013
The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
false
false
_11_6_
0
135
7
In stock
false
false
Barry Canton
annotation1721522
1
Initiation Region
range1721522
1
12
23
annotation1721521
1
Polymerase Binding Region
range1721521
1
1
11
annotation1721520
1
Transcription Start Site
range1721520
1
18
18
BBa_J31001
1
Hin LVA
DNA invertase Hin tagged with LVA
2006-07-11T11:00:00Z
2015-08-31T04:08:45Z
Salmonella typhimurium and the the Hin part without LVA.
This part produces the Hin protein with an LVA degredation tag. We are currently looking into this as a way to ensure no Hin is floating around once we turn off the Hin promoter.
false
false
_61_
0
918
61
In stock
true
None really
false
Erin Zwack, Sabriya Rosemond
annotation1910564
1
Hin
range1910564
1
1
570
annotation1910565
1
Fwd primer J31001
range1910565
1
1
3
annotation1910570
1
Rev primer J31001
range1910570
1
551
612
annotation1910567
1
Stop codon
range1910567
1
610
612
annotation1910566
1
LVA
range1910566
1
571
609
BBa_I715023
1
RFP2
Carboxyl portion of RFP
2007-06-24T11:00:00Z
2016-01-25T04:44:49Z
Sequence from part BBa_E1010.
The second portion of the RFP gene, after the HixC insertion point.
false
false
_120_
4206
201
61
In stock
false
Used web tool.
true
Andrew Martens
annotation1935384
1
(two in tandem)
range1935384
1
215
219
annotation1935383
1
Continue coding sequence
range1935383
1
2
214
annotation1935382
1
extra base (avoid frameshift)
range1935382
1
1
1
BBa_I715022
1
RFP1
Amino Portion of RFP
2007-06-24T11:00:00Z
2016-01-25T04:43:58Z
adf
Released HQ 2013
af
false
false
_120_
4206
201
61
In stock
false
afd
true
Andrew Martens
annotation1935381
1
Coding sequence
range1935381
1
1
462
annotation1935380
1
start
range1935380
1
1
3
BBa_J44000
1
hixC
hixC binding site for Salmonella typhimurium Hin recombinase
2006-06-05T11:00:00Z
2015-08-31T04:08:48Z
Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase
[http://www.genetics.org/cgi/content/abstract/149/4/1649]
A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
false
true
_71_
0
606
61
In stock
true
Standard BioBrick prefix and suffix were added to the 26 bp sequence.
true
Missouri Western and Davidson Groups, Todd Eckdahl
BBa_I715019
1
GFP1
Amino Half of GFP (aka GFP1)
2007-06-21T11:00:00Z
2015-08-31T04:07:49Z
A
Released HQ 2013
A
false
false
_120_
0
1491
9
In stock
false
A
true
Will DeLoache
annotation1935357
1
Coding Amino Portion GFP (aka GFP1)
range1935357
1
1
471
annotation1935356
1
Start Codon
range1935356
1
1
3
BBa_I715020
1
GFP2
Carboxyl Half of GFP (aka GFP2)
2007-06-21T11:00:00Z
2015-08-31T04:07:49Z
A
Released HQ 2013
A
false
false
_120_
0
1491
9
In stock
false
A
true
Will DeLoache
annotation1935361
1
Coding Carboxyl half of GFP (aka GFP2)
range1935361
1
1
244
annotation1935362
1
Stop Codons (two in tandem)
range1935362
1
245
250
annotation1935360
1
extra base to maintain reading frame
range1935360
1
1
1
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961227
1
start
range1961227
1
173
173
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_I715058_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagactactagagttatcaaaaaccatggtttttgataatactagagtaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagactactagagttatcaaaaaccatggtttttgataatactagagtaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatcaaaaaccatggtttttgataatactagagtggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaatactagagttatcaaaaaccatggtttttgataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0085_sequence
1
taatacgactcactatagggaga
BBa_I715023_sequence
1
tggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_I715019_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
BBa_J44000_sequence
1
ttatcaaaaaccatggtttttgataa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_I715020_sequence
1
taagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_J31001_sequence
1
atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I715022_sequence
1
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z