BBa_I715058
type: ComponentDefinition
persistent identity: BBa I715058
display id: BBa_I715058
version: 1
was generated by: iGEM to SBOL conversion
description: HPP - B1 (DBA) + Hin
created: 2007-11-25T12:00:00Z
modified: 2015-08-31T04:07:50Z
mutable provenance: See subparts.
owned by: james
owned by: myers
mutable description: This part has our DBA construct downtream of the Hin expression cassette.
discontinued: false
dominant: false
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Will DeLoache
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J44000
component: BBa_I715022
component: BBa_B0034
component: BBa_I715020
component: BBa_B0012
component: BBa_B0010
component: BBa_I715019
component: BBa_I715023
component: BBa_R0010
component: BBa_J31001
component: BBa_B0030
component: BBa_R0085
sequence annotation: BBa_R0010
sequence annotation: BBa_B0030
sequence annotation: BBa_J31001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0085
sequence annotation: BBa_B0034
sequence annotation: BBa_I715022
sequence annotation: BBa_J44000
sequence annotation: BBa_I715020
sequence annotation: BBa_I715023
sequence annotation: BBa_I715019
sequence: https://synbiohub.org/public/igem/BBa_I715058_sequence/1

BBa_J44000
type: Component
persistent identity: component1958786
display id: component1958786
version: 1
top level: BBa_I715058
definition: hixC
access: public

BBa_I715022
type: Component
persistent identity: component1958785
display id: component1958785
version: 1
top level: BBa_I715058
definition: RFP1
access: public

BBa_B0034
type: Component
persistent identity: component1958782
display id: component1958782
version: 1
top level: BBa_I715058
definition: BBa_B0034
access: public

BBa_I715020
type: Component
persistent identity: component1958780
display id: component1958780
version: 1
top level: BBa_I715058
definition: GFP2
access: public

BBa_J44000
type: Component
persistent identity: component1958796
display id: component1958796
version: 1
top level: BBa_I715058
definition: hixC
access: public

BBa_B0012
type: Component
persistent identity: component1958792
display id: component1958792
version: 1
top level: BBa_I715058
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1958790
display id: component1958790
version: 1
top level: BBa_I715058
definition: BBa_B0010
access: public

BBa_I715020
type: Component
persistent identity: component1958789
display id: component1958789
version: 1
top level: BBa_I715058
definition: GFP2
access: public

BBa_J44000
type: Component
persistent identity: component1958805
display id: component1958805
version: 1
top level: BBa_I715058
definition: hixC
access: public

BBa_I715019
type: Component
persistent identity: component1958804
display id: component1958804
version: 1
top level: BBa_I715058
definition: GFP1
access: public

BBa_B0034
type: Component
persistent identity: component1958801
display id: component1958801
version: 1
top level: BBa_I715058
definition: BBa_B0034
access: public

BBa_I715023
type: Component
persistent identity: component1958799
display id: component1958799
version: 1
top level: BBa_I715058
definition: RFP2
access: public

BBa_R0010
type: Component
persistent identity: component1958746
display id: component1958746
version: 1
top level: BBa_I715058
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1958764
display id: component1958764
version: 1
top level: BBa_I715058
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1958762
display id: component1958762
version: 1
top level: BBa_I715058
definition: BBa_B0010
access: public

BBa_J31001
type: Component
persistent identity: component1958761
display id: component1958761
version: 1
top level: BBa_I715058
definition: Hin LVA
access: public

BBa_B0030
type: Component
persistent identity: component1958754
display id: component1958754
version: 1
top level: BBa_I715058
definition: BBa_B0030
access: public

BBa_J44000
type: Component
persistent identity: component1958777
display id: component1958777
version: 1
top level: BBa_I715058
definition: hixC
access: public

BBa_I715022
type: Component
persistent identity: component1958776
display id: component1958776
version: 1
top level: BBa_I715058
definition: RFP1
access: public

BBa_B0034
type: Component
persistent identity: component1958773
display id: component1958773
version: 1
top level: BBa_I715058
definition: BBa_B0034
access: public

BBa_R0085
type: Component
persistent identity: component1958771
display id: component1958771
version: 1
top level: BBa_I715058
definition: BBa_R0085
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1958746
display id: annotation1958746
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958746/range1958746/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I715058/annotation1958746/range1958746/1
type: Range
persistent identity: range1958746
display id: range1958746
version: 1
top level: BBa_I715058
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1958754
display id: annotation1958754
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958754/range1958754/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_I715058/annotation1958754/range1958754/1
type: Range
persistent identity: range1958754
display id: range1958754
version: 1
top level: BBa_I715058
direction: direction/forward
start: 209
end: 223
orientation: inline

BBa_J31001
type: SequenceAnnotation
persistent identity: annotation1958761
display id: annotation1958761
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958761/range1958761/1
component: BBa_J31001

https://synbiohub.org/public/igem/BBa_I715058/annotation1958761/range1958761/1
type: Range
persistent identity: range1958761
display id: range1958761
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 230
end: 841
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1958762
display id: annotation1958762
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958762/range1958762/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715058/annotation1958762/range1958762/1
type: Range
persistent identity: range1958762
display id: range1958762
version: 1
top level: BBa_I715058
direction: direction/forward
start: 850
end: 929
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1958764
display id: annotation1958764
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958764/range1958764/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715058/annotation1958764/range1958764/1
type: Range
persistent identity: range1958764
display id: range1958764
version: 1
top level: BBa_I715058
direction: direction/forward
start: 938
end: 978
orientation: inline

BBa_R0085
type: SequenceAnnotation
persistent identity: annotation1958771
display id: annotation1958771
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958771/range1958771/1
component: BBa_R0085

https://synbiohub.org/public/igem/BBa_I715058/annotation1958771/range1958771/1
type: Range
persistent identity: range1958771
display id: range1958771
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 987
end: 1009
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1958773
display id: annotation1958773
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958773/range1958773/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715058/annotation1958773/range1958773/1
type: Range
persistent identity: range1958773
display id: range1958773
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 1018
end: 1029
orientation: inline

BBa_I715022
type: SequenceAnnotation
persistent identity: annotation1958776
display id: annotation1958776
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958776/range1958776/1
component: BBa_I715022

https://synbiohub.org/public/igem/BBa_I715058/annotation1958776/range1958776/1
type: Range
persistent identity: range1958776
display id: range1958776
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 1036
end: 1497
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1958777
display id: annotation1958777
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958777/range1958777/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715058/annotation1958777/range1958777/1
type: Range
persistent identity: range1958777
display id: range1958777
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 1506
end: 1531
orientation: inline

BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1958780
display id: annotation1958780
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958780/range1958780/1
component: BBa_I715020

https://synbiohub.org/public/igem/BBa_I715058/annotation1958780/range1958780/1
type: Range
persistent identity: range1958780
display id: range1958780
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 1540
end: 1789
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1958782
display id: annotation1958782
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958782/range1958782/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715058/annotation1958782/range1958782/1
type: Range
persistent identity: range1958782
display id: range1958782
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 1798
end: 1809
orientation: inline

BBa_I715022
type: SequenceAnnotation
persistent identity: annotation1958785
display id: annotation1958785
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958785/range1958785/1
component: BBa_I715022

https://synbiohub.org/public/igem/BBa_I715058/annotation1958785/range1958785/1
type: Range
persistent identity: range1958785
display id: range1958785
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 1816
end: 2277
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1958786
display id: annotation1958786
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958786/range1958786/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715058/annotation1958786/range1958786/1
type: Range
persistent identity: range1958786
display id: range1958786
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 2286
end: 2311
orientation: inline

BBa_I715020
type: SequenceAnnotation
persistent identity: annotation1958789
display id: annotation1958789
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958789/range1958789/1
component: BBa_I715020

https://synbiohub.org/public/igem/BBa_I715058/annotation1958789/range1958789/1
type: Range
persistent identity: range1958789
display id: range1958789
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 2320
end: 2569
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1958790
display id: annotation1958790
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958790/range1958790/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I715058/annotation1958790/range1958790/1
type: Range
persistent identity: range1958790
display id: range1958790
version: 1
top level: BBa_I715058
direction: direction/forward
start: 2578
end: 2657
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1958792
display id: annotation1958792
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958792/range1958792/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I715058/annotation1958792/range1958792/1
type: Range
persistent identity: range1958792
display id: range1958792
version: 1
top level: BBa_I715058
direction: direction/forward
start: 2666
end: 2706
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1958796
display id: annotation1958796
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958796/range1958796/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715058/annotation1958796/range1958796/1
type: Range
persistent identity: range1958796
display id: range1958796
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 2715
end: 2740
orientation: inline

BBa_I715023
type: SequenceAnnotation
persistent identity: annotation1958799
display id: annotation1958799
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958799/range1958799/1
component: BBa_I715023

https://synbiohub.org/public/igem/BBa_I715058/annotation1958799/range1958799/1
type: Range
persistent identity: range1958799
display id: range1958799
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 2749
end: 2968
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1958801
display id: annotation1958801
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958801/range1958801/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715058/annotation1958801/range1958801/1
type: Range
persistent identity: range1958801
display id: range1958801
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 2977
end: 2988
orientation: inline

BBa_I715019
type: SequenceAnnotation
persistent identity: annotation1958804
display id: annotation1958804
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958804/range1958804/1
component: BBa_I715019

https://synbiohub.org/public/igem/BBa_I715058/annotation1958804/range1958804/1
type: Range
persistent identity: range1958804
display id: range1958804
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 2995
end: 3465
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation1958805
display id: annotation1958805
version: 1
top level: BBa_I715058
location: https://synbiohub.org/public/igem/BBa_I715058/annotation1958805/range1958805/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715058/annotation1958805/range1958805/1
type: Range
persistent identity: range1958805
display id: range1958805
version: 1
top level: BBa_I715058
direction: direction/reverse
start: 3474
end: 3499
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP1
type: ComponentDefinition
persistent identity: BBa I715019
display id: BBa_I715019
version: 1
was generated by: iGEM to SBOL conversion
description: Amino Half of GFP (aka GFP1)
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
mutable provenance: A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: A
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: A
star: true
creator: Will DeLoache
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: Coding Amino Portion GFP (aka GFP1)
sequence annotation: Start Codon
sequence: https://synbiohub.org/public/igem/BBa_I715019_sequence/1

Coding Amino Portion GFP (aka GFP1)
type: SequenceAnnotation
persistent identity: annotation1935357
display id: annotation1935357
version: 1
top level: GFP1
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715019/annotation1935357/range1935357/1
type: Range
persistent identity: range1935357
display id: range1935357
version: 1
top level: GFP1
direction: direction/forward
start: 1
end: 471
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation1935356
display id: annotation1935356
version: 1
top level: GFP1
location: https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I715019/annotation1935356/range1935356/1
type: Range
persistent identity: range1935356
display id: range1935356
version: 1
top level: GFP1
direction: direction/forward
start: 1
end: 3
orientation: inline

GFP2
type: ComponentDefinition
persistent identity: BBa I715020
display id: BBa_I715020
version: 1
was generated by: iGEM to SBOL conversion
description: Carboxyl Half of GFP (aka GFP2)
created: 2007-06-21T11:00:00Z
modified: 2015-08-31T04:07:49Z
mutable provenance: A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: A
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 0
owner id: 1491
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: A
star: true
creator: Will DeLoache
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Coding Carboxyl half of GFP (aka GFP2)
sequence annotation: extra base to maintain reading frame
sequence annotation: Stop Codons (two in tandem)
sequence: https://synbiohub.org/public/igem/BBa_I715020_sequence/1

Coding Carboxyl half of GFP (aka GFP2)
type: SequenceAnnotation
persistent identity: annotation1935361
display id: annotation1935361
version: 1
top level: GFP2
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715020/annotation1935361/range1935361/1
type: Range
persistent identity: range1935361
display id: range1935361
version: 1
top level: GFP2
direction: direction/forward
start: 1
end: 244
orientation: inline

extra base to maintain reading frame
type: SequenceAnnotation
persistent identity: annotation1935360
display id: annotation1935360
version: 1
top level: GFP2
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I715020/annotation1935360/range1935360/1
type: Range
persistent identity: range1935360
display id: range1935360
version: 1
top level: GFP2
direction: direction/forward
start: 1
end: 1
orientation: inline

Stop Codons (two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935362
display id: annotation1935362
version: 1
top level: GFP2
location: https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I715020/annotation1935362/range1935362/1
type: Range
persistent identity: range1935362
display id: range1935362
version: 1
top level: GFP2
direction: direction/forward
start: 245
end: 250
orientation: inline

RFP1
type: ComponentDefinition
persistent identity: BBa I715022
display id: BBa_I715022
version: 1
was generated by: iGEM to SBOL conversion
description: Amino Portion of RFP
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:43:58Z
mutable provenance: adf
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: af
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
sample status: In stock
status: status/Available
bookmark: false
mutable notes: afd
star: true
creator: Andrew Martens
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding sequence
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_I715022_sequence/1

Coding sequence
type: SequenceAnnotation
persistent identity: annotation1935381
display id: annotation1935381
version: 1
top level: RFP1
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715022/annotation1935381/range1935381/1
type: Range
persistent identity: range1935381
display id: range1935381
version: 1
top level: RFP1
direction: direction/forward
start: 1
end: 462
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1935380
display id: annotation1935380
version: 1
top level: RFP1
location: https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I715022/annotation1935380/range1935380/1
type: Range
persistent identity: range1935380
display id: range1935380
version: 1
top level: RFP1
direction: direction/forward
start: 1
end: 3
orientation: inline

RFP2
type: ComponentDefinition
persistent identity: BBa I715023
display id: BBa_I715023
version: 1
was generated by: iGEM to SBOL conversion
description: Carboxyl portion of RFP
created: 2007-06-24T11:00:00Z
modified: 2016-01-25T04:44:49Z
mutable provenance: Sequence from part BBa_E1010.
owned by: james
owned by: myers
mutable description: The second portion of the RFP gene, after the HixC insertion point.
discontinued: false
dominant: false
experience: experience/Works
group u list: _120_
m user id: 4206
owner id: 201
owning group id: 61
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Used web tool.
star: true
creator: Andrew Martens
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: extra base (avoid frameshift)
sequence annotation: Continue coding sequence
sequence annotation: (two in tandem)
sequence: https://synbiohub.org/public/igem/BBa_I715023_sequence/1

extra base (avoid frameshift)
type: SequenceAnnotation
persistent identity: annotation1935382
display id: annotation1935382
version: 1
top level: RFP2
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I715023/annotation1935382/range1935382/1
type: Range
persistent identity: range1935382
display id: range1935382
version: 1
top level: RFP2
direction: direction/forward
start: 1
end: 1
orientation: inline

Continue coding sequence
type: SequenceAnnotation
persistent identity: annotation1935383
display id: annotation1935383
version: 1
top level: RFP2
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I715023/annotation1935383/range1935383/1
type: Range
persistent identity: range1935383
display id: range1935383
version: 1
top level: RFP2
direction: direction/forward
start: 2
end: 214
orientation: inline

(two in tandem)
type: SequenceAnnotation
persistent identity: annotation1935384
display id: annotation1935384
version: 1
top level: RFP2
location: https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I715023/annotation1935384/range1935384/1
type: Range
persistent identity: range1935384
display id: range1935384
version: 1
top level: RFP2
direction: direction/forward
start: 215
end: 219
orientation: inline

Hin LVA
type: ComponentDefinition
persistent identity: BBa J31001
display id: BBa_J31001
version: 1
was generated by: iGEM to SBOL conversion
description: DNA invertase Hin tagged with LVA
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: Salmonella typhimurium and the the Hin part without LVA.
owned by: james
owned by: myers
mutable description: This part produces the Hin protein with an LVA degredation tag. We are currently looking into this as a way to ensure no Hin is floating around once we turn off the Hin promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: None really
star: false
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Fwd primer J31001
sequence annotation: Hin
sequence annotation: Rev primer J31001
sequence annotation: LVA
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_J31001_sequence/1

Fwd primer J31001
type: SequenceAnnotation
persistent identity: annotation1910565
display id: annotation1910565
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31001/annotation1910565/range1910565/1
type: Range
persistent identity: range1910565
display id: range1910565
version: 1
top level: Hin LVA
direction: direction/forward
start: 1
end: 3
orientation: inline

Hin
type: SequenceAnnotation
persistent identity: annotation1910564
display id: annotation1910564
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31001/annotation1910564/range1910564/1
type: Range
persistent identity: range1910564
display id: range1910564
version: 1
top level: Hin LVA
direction: direction/forward
start: 1
end: 570
orientation: inline

Rev primer J31001
type: SequenceAnnotation
persistent identity: annotation1910570
display id: annotation1910570
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31001/annotation1910570/range1910570/1
type: Range
persistent identity: range1910570
display id: range1910570
version: 1
top level: Hin LVA
direction: direction/reverse
start: 551
end: 612
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1910566
display id: annotation1910566
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J31001/annotation1910566/range1910566/1
type: Range
persistent identity: range1910566
display id: range1910566
version: 1
top level: Hin LVA
direction: direction/forward
start: 571
end: 609
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation1910567
display id: annotation1910567
version: 1
top level: Hin LVA
location: https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J31001/annotation1910567/range1910567/1
type: Range
persistent identity: range1910567
display id: range1910567
version: 1
top level: Hin LVA
direction: direction/forward
start: 610
end: 612
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: iGEM to SBOL conversion
description: hixC binding site for Salmonella typhimurium Hin recombinase
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
star: true
creator: Missouri Western and Davidson Groups, Todd Eckdahl
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1

BBa_R0085
type: ComponentDefinition
persistent identity: BBa R0085
display id: BBa_R0085
version: 1
was generated by: iGEM to SBOL conversion
description: T7 Consensus Promoter Sequence
created: 2005-02-21T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Sequence obtained from Sri Kosuri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The T7 promoter should only produce PoPS when the T7 polymerase is also being expressed.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Polymerase Binding Region
sequence annotation: Initiation Region
sequence annotation: Transcription Start Site
sequence: https://synbiohub.org/public/igem/BBa_R0085_sequence/1

Polymerase Binding Region
type: SequenceAnnotation
persistent identity: annotation1721521
display id: annotation1721521
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0085/annotation1721521/range1721521/1
type: Range
persistent identity: range1721521
display id: range1721521
version: 1
top level: BBa_R0085
direction: direction/forward
start: 1
end: 11
orientation: inline

Initiation Region
type: SequenceAnnotation
persistent identity: annotation1721522
display id: annotation1721522
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0085/annotation1721522/range1721522/1
type: Range
persistent identity: range1721522
display id: range1721522
version: 1
top level: BBa_R0085
direction: direction/forward
start: 12
end: 23
orientation: inline

Transcription Start Site
type: SequenceAnnotation
persistent identity: annotation1721520
display id: annotation1721520
version: 1
top level: BBa_R0085
location: https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R0085/annotation1721520/range1721520/1
type: Range
persistent identity: range1721520
display id: range1721520
version: 1
top level: BBa_R0085
direction: direction/forward
start: 18
end: 18
orientation: inline

https://synbiohub.org/public/igem/BBa_I715058_sequence/1
type: Sequence
persistent identity: BBa I715058 sequence
display id: BBa_I715058_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagactactagagttatcaaaaaccatggtttttgataatactagagtaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715019_sequence/1
type: Sequence
persistent identity: BBa I715019 sequence
display id: BBa_I715019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715020_sequence/1
type: Sequence
persistent identity: BBa I715020 sequence
display id: BBa_I715020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715022_sequence/1
type: Sequence
persistent identity: BBa I715022 sequence
display id: BBa_I715022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I715023_sequence/1
type: Sequence
persistent identity: BBa I715023 sequence
display id: BBa_I715023_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31001_sequence/1
type: Sequence
persistent identity: BBa J31001 sequence
display id: BBa_J31001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttaccagtgcaaattgtgaccgcatttttgaggaccgtatcagtggcaagattgcaaaccgccccggcctgaaacgagcgttaaagtatgtaaataaaggcgatactcttgtcgtctggaaattagacagactgggccgcagcgtgaaaaacctggtggcgttaatatcagaattacatgaacgtggagctcacttccattctttaaccgatagtattgataccagtagcgcgatggggcgattcttttttcatgtaatgtcagcactggccgagatggagcgagaattaattgtcgagcgaacccttgccggactggctgccgccagagcgcaaggacgactgggagggcgccctcgggcgatcaacagacatgaacaggaacagattagtcggctattagagaaaggccatcctcggcagcaactagctattatttttggtattggcgtatctaccttatacagatattttccggcaagccgtataaaaaaacgaatgaataggcctgctgcaaacgacgaaaactacgctttagtagcttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44000_sequence/1
type: Sequence
persistent identity: BBa J44000 sequence
display id: BBa_J44000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0085_sequence/1
type: Sequence
persistent identity: BBa R0085 sequence
display id: BBa_R0085_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z