##gff-version 3
##sequence-region BBa_I715059 1 1194
BBa_I715059	.	promoter	139	272	.	+	0	ID=BBa_I715052;Name=BBa_I715052
BBa_I715059	.	CDS	158	202	.	+	0	ID=annotation1958523;Name=leader peptide;Parent=BBa_I715052
BBa_I715059	.	conserved_region	185	190	.	+	0	ID=annotation1958521;Name=tandem trp codons;Parent=BBa_I715052
BBa_I715059	.	sequence_feature	265	272	.	+	0	ID=annotation1958524;Name=Poly T;Parent=BBa_I715052
BBa_I715059	.	ribosome_entry_site	143	151	.	+	0	ID=annotation1958519;Name=Predicted RBS;Parent=BBa_I715052
BBa_I715059	.	start_codon	158	160	.	+	0	ID=annotation1958520;Name=start;Parent=BBa_I715052
BBa_I715059	.	stop_codon	200	202	.	+	0	ID=annotation1958522;Name=stop;Parent=BBa_I715052
BBa_I715059	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
BBa_I715059	.	terminator	1066	1145	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I715059	.	stem_loop	1077	1120	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I715059	.	engineered_region	1066	1145	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I715059	.	engineered_region	299	1057	.	+	0	ID=BBa_J04031;Name=BBa_J04031
BBa_I715059	.	engineered_region	299	1018	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_I715059	.	tag	1013	1051	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_I715059	.	stop_codon	1052	1057	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_I715059	.	start_codon	299	301	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_I715059	.	terminator	1154	1194	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I715059	.	engineered_region	1154	1194	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I715059	.	stop_codon	1187	1187	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I715059	.	stem_loop	1161	1180	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I715059	.	polyA_site	1181	1194	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I715059	.	ribosome_entry_site	281	292	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I715059	.	conserved_region	285	288	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_I715059
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcgtaaaaagggtatcgacaa
tgaaagcaattttcgtactgaaaggttggtggcgcacttcctgaaacgggcagtgtattcaccatgcgtaaagcaatca
gatacccagcccgcctaatgagcgggctttttttttactagagaaagaggagaaatactagatgcgtaaaggagaagaa
cttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagg
gtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaac
acttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaag
tcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgg
acacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaac
ttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatg
gccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagacca
catggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaac
gacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttata