LOCUS BBa_I715060 1219 bp DNA linear 26-Dec-2024 DEFINITION pBad-trp/hixC-RBS-GFPlva-TT ACCESSION BBa_I715060 VERSION BBa_I715060.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 158..160 /label=start RBS 143..151 /label=predicted RBS CDS 158..202 /label=leader peptide misc_feature 185..190 /label=tandem trp codons misc_feature 200..202 /label=stop misc_feature 290..297 /label=Poly T misc_feature 229..254 /label=hixC promoter 139..297 /label=BBa_I715053 misc_feature 310..313 /label=conserved RBS 306..317 /label=BBa_B0034 misc_feature 324..1043 /label=BBa_E0040 misc_feature 1038..1076 /label=LVA misc_feature 1077..1082 /label=Double Stop misc_feature 324..326 /label=Start misc_feature 324..1082 /label=BBa_J04031 stem_loop 1102..1145 /label=stem_loop misc_feature 1091..1170 /label=BBa_B0010 terminator 1091..1170 /label=BBa_B0010 stem_loop 1186..1205 /label=T7 TE misc_feature 1212..1212 /label=stop polyA_site 1206..1219 /label=polya misc_feature 1179..1219 /label=BBa_B0012 terminator 1179..1219 /label=BBa_B0012 misc_feature 306..1219 /label=BBa_S03903 misc_feature 139..1219 /label=BBa_S03913 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagcg taaaaagggt atcgacaatg aaagcaattt tcgtactgaa 181 aggttggtgg cgcacttcct gaaacgggca gtgtattcac catgcgtatt atcaaaaacc 241 atggtttttg ataaagcaat cagataccca gcccgcctaa tgagcgggct ttttttttac 301 tagagaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc 361 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg 421 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac 481 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga 541 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg 601 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca 661 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag 721 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca 781 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 841 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 901 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 961 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 1021 tggatgaact atacaaaagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat 1081 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 1141 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 1201 gggcctttct gcgtttata //