##gff-version 3
##sequence-region BBa_I715060 1 1219
BBa_I715060	.	engineered_region	139	1219	.	+	0	ID=BBa_S03913;Name=BBa_S03913
BBa_I715060	.	engineered_region	306	1219	.	+	0	ID=BBa_S03903;Name=BBa_S03903;Parent=BBa_S03913
BBa_I715060	.	terminator	1091	1170	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03903
BBa_I715060	.	stem_loop	1102	1145	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I715060	.	engineered_region	1091	1170	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I715060	.	engineered_region	324	1082	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_S03903
BBa_I715060	.	engineered_region	324	1043	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_I715060	.	tag	1038	1076	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_I715060	.	stop_codon	1077	1082	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_I715060	.	start_codon	324	326	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_I715060	.	terminator	1179	1219	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03903
BBa_I715060	.	engineered_region	1179	1219	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I715060	.	stop_codon	1212	1212	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I715060	.	stem_loop	1186	1205	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I715060	.	polyA_site	1206	1219	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I715060	.	ribosome_entry_site	306	317	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03903
BBa_I715060	.	conserved_region	310	313	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715060	.	promoter	139	297	.	+	0	ID=BBa_I715053;Name=BBa_I715053;Parent=BBa_S03913
BBa_I715060	.	ribosome_entry_site	143	151	.	+	0	ID=annotation1958526;Name=predicted RBS;Parent=BBa_I715053
BBa_I715060	.	sequence_feature	290	297	.	+	0	ID=annotation1958530;Name=Poly T;Parent=BBa_I715053
BBa_I715060	.	stop_codon	200	202	.	+	0	ID=annotation1958529;Name=stop;Parent=BBa_I715053
BBa_I715060	.	conserved_region	185	190	.	+	0	ID=annotation1958528;Name=tandem trp codons;Parent=BBa_I715053
BBa_I715060	.	start_codon	158	160	.	+	0	ID=annotation1958525;Name=start;Parent=BBa_I715053
BBa_I715060	.	sequence_feature	229	254	.	+	0	ID=annotation1958531;Name=hixC;Parent=BBa_I715053
BBa_I715060	.	CDS	158	202	.	+	0	ID=annotation1958527;Name=leader peptide;Parent=BBa_I715053
BBa_I715060	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
>BBa_I715060
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcgtaaaaagggtatcgacaa
tgaaagcaattttcgtactgaaaggttggtggcgcacttcctgaaacgggcagtgtattcaccatgcgtattatcaaaa
accatggtttttgataaagcaatcagatacccagcccgcctaatgagcgggctttttttttactagagaaagaggagaa
atactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatggg
cacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactg
gaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatca
tatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgac
gggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatt
ttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaa
acaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattat
caacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcga
aagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatga
actatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata