LOCUS       BBa_I715061             1225 bp    DNA     linear       22-Dec-2024
DEFINITION  PLAC-Trp-RBS-GFP-TT
ACCESSION   BBa_I715061
VERSION     BBa_I715061.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             213..221
                     /label=Predicted RBS
     misc_feature    228..230
                     /label=start
     misc_feature    255..260
                     /label=tandem trp codons
     misc_feature    270..272
                     /label=stop
     CDS             228..272
                     /label=leader peptide
     misc_feature    335..342
                     /label=Poly T
     promoter        209..342
                     /label=BBa_I715052
     misc_feature    355..358
                     /label=conserved
     RBS             351..362
                     /label=BBa_B0034
     CDS             369..1088
                     /label=GFP protein
     CDS             369..1088
                     /label=BBa_E0040
     stem_loop       1108..1151
                     /label=stem_loop
     misc_feature    1097..1176
                     /label=BBa_B0010
     terminator      1097..1176
                     /label=BBa_B0010
     stem_loop       1192..1211
                     /label=T7 TE
     misc_feature    1218..1218
                     /label=stop
     polyA_site      1212..1225
                     /label=polya
     misc_feature    1185..1225
                     /label=BBa_B0012
     terminator      1185..1225
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagcg taaaaagggt atcgacaatg aaagcaattt
      241 tcgtactgaa aggttggtgg cgcacttcct gaaacgggca gtgtattcac catgcgtaaa
      301 gcaatcagat acccagcccg cctaatgagc gggctttttt tttactagag aaagaggaga
      361 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      421 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      481 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      541 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
      601 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      661 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
      721 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
      781 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
      841 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
      901 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
      961 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1021 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1081 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1141 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1201 tcgggtgggc ctttctgcgt ttata
//