LOCUS       BBa_I715063             1155 bp    DNA     linear       22-Nov-2024
DEFINITION  PBAD-Trp-RBS-GFP-TT
ACCESSION   BBa_I715063
VERSION     BBa_I715063.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     RBS             143..151
                     /label=Predicted RBS
     misc_feature    158..160
                     /label=start
     misc_feature    185..190
                     /label=tandem trp codons
     misc_feature    200..202
                     /label=stop
     CDS             158..202
                     /label=leader peptide
     misc_feature    265..272
                     /label=Poly T
     promoter        139..272
                     /label=BBa_I715052
     misc_feature    285..288
                     /label=conserved
     RBS             281..292
                     /label=BBa_B0034
     CDS             299..1018
                     /label=GFP protein
     CDS             299..1018
                     /label=BBa_E0040
     stem_loop       1038..1081
                     /label=stem_loop
     misc_feature    1027..1106
                     /label=BBa_B0010
     terminator      1027..1106
                     /label=BBa_B0010
     stem_loop       1122..1141
                     /label=T7 TE
     misc_feature    1148..1148
                     /label=stop
     polyA_site      1142..1155
                     /label=polya
     misc_feature    1115..1155
                     /label=BBa_B0012
     terminator      1115..1155
                     /label=BBa_B0012
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagcg taaaaagggt atcgacaatg aaagcaattt tcgtactgaa
      181 aggttggtgg cgcacttcct gaaacgggca gtgtattcac catgcgtaaa gcaatcagat
      241 acccagcccg cctaatgagc gggctttttt tttactagag aaagaggaga aatactagat
      301 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      361 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      421 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
      481 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
      541 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
      601 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
      661 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
      721 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
      781 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
      841 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
      901 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
      961 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata
     1021 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1081 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1141 ctttctgcgt ttata
//