LOCUS       BBa_I715065             2321 bp    DNA     linear       22-Dec-2024
DEFINITION  Burnt Pancake Tester with RE and backwards Tet resistance
ACCESSION   BBa_I715065
VERSION     BBa_I715065.1  
FEATURES             Location/Qualifiers
     CDS             1..1191
                     /label=tetA(C) backwards
     misc_feature    1..3
                     /label=stop
     primer_bind     1..20
                     /label=Rev primer J31006
     primer_bind     1168..1191
                     /label=Fwd primer J31006
     CDS             1..1191
                     /label=BBa_J31006
     RBS             1200..1214
                     /label=Reverse RBS
     RBS             1200..1214
                     /label=BBa_J44001
     misc_feature    1223..1248
                     /label=BBa_J44000
     CDS             1257..1344
                     /label=end of LacI coding region (inactive)
     misc_feature    1257..1456
                     /label=BBa_R0010
     misc_feature    1345..1382
                     /label=CAP binding site
     promoter        1393..1398
                     /label=-35
     promoter        1417..1422
                     /label=-10
     misc_feature    1422..1456
                     /label=LacI binding site
     misc_feature    1429..1429
                     /label=start
     promoter        1257..1456
                     /label=BBa_R0010
     misc_feature    1465..1490
                     /label=BBa_J44000
     misc_feature    1503..1506
                     /label=conserved
     RBS             1499..1510
                     /label=BBa_B0034
     CDS             1517..2236
                     /label=GFP protein
     CDS             1517..2236
                     /label=BBa_E0040
     misc_feature    2295..2300
                     /label=Former SpeI site
     misc_feature    2295..2295
                     /label=Mutation of SpeI site
     misc_feature    2245..2321
                     /label=RE Sequence
     misc_feature    2273..2273
                     /label=Originally a C
     misc_feature    2321..2321
                     /label=Insertion right before biobrick ends
     misc_feature    2250..2265
                     /label=Proximal Fis Binding Site
     misc_feature    2299..2313
                     /label=Distal Fis Binding Site
     misc_feature    2245..2321
                     /label=BBa_J3101
ORIGIN
        1 ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
       61 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
      121 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
      181 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
      241 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
      301 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
      361 cttctcgccg aaacgtttgg tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa
      421 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
      481 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
      541 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
      601 gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca
      661 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
      721 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
      781 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
      841 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
      901 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
      961 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     1021 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     1081 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     1141 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca ttactagagt
     1201 ttctcctctt taattactag agttatcaaa aaccatggtt tttgataata ctagagcaat
     1261 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1321 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1381 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1441 ataacaattt cacacatact agagttatca aaaaccatgg tttttgataa tactagagaa
     1501 agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg tcccaattct
     1561 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     1621 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     1681 tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg cgagataccc
     1741 agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     1801 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     1861 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     1921 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     1981 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     2041 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     2101 accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag
     2161 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     2221 actatacaaa taataatact agagttcggg tgtcaacaat tgaccaaaat attgatttac
     2281 agcgtaatgc gctttctagt gcaaattgtg accgcatttt g
//