##gff-version 3
##sequence-region BBa_I715065 1 2321
BBa_I715065	.	ribosome_entry_site	1200	1214	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_I715065	.	ribosome_entry_site	1200	1214	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_I715065	.	promoter	1257	1456	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I715065	.	non_covalent_binding_site	1345	1382	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I715065	.	engineered_region	1257	1456	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I715065	.	promoter	1393	1398	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I715065	.	promoter	1417	1422	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I715065	.	start_codon	1429	1429	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I715065	.	non_covalent_binding_site	1422	1456	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I715065	.	CDS	1257	1344	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I715065	.	sequence_feature	1223	1248	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715065	.	sequence_feature	2245	2321	.	+	0	ID=BBa_J3101;Name=BBa_J3101
BBa_I715065	.	non_covalent_binding_site	2250	2265	.	+	0	ID=annotation1884991;Name=Proximal Fis Binding Site;Parent=BBa_J3101
BBa_I715065	.	non_covalent_binding_site	2299	2313	.	+	0	ID=annotation1884992;Name=Distal Fis Binding Site;Parent=BBa_J3101
BBa_I715065	.	sequence_feature	2295	2300	.	+	0	ID=annotation1880471;Name=Former SpeI site;Parent=BBa_J3101
BBa_I715065	.	sequence_feature	2245	2321	.	+	0	ID=annotation1884988;Name=RE Sequence;Parent=BBa_J3101
BBa_I715065	.	sequence_feature	2321	2321	.	+	0	ID=annotation1884990;Name=Insertion right before biobrick ends;Parent=BBa_J3101
BBa_I715065	.	sequence_feature	2273	2273	.	+	0	ID=annotation1884989;Name=Originally a C;Parent=BBa_J3101
BBa_I715065	.	sequence_feature	2295	2295	.	+	0	ID=annotation1880472;Name=Mutation of SpeI site;Parent=BBa_J3101
BBa_I715065	.	CDS	1	1191	.	+	0	ID=BBa_J31006;Name=BBa_J31006
BBa_I715065	.	CDS	1	1191	.	+	0	ID=annotation1884998;Name=tetA(C) backwards;Parent=BBa_J31006
BBa_I715065	.	primer_binding_site	1168	1191	.	+	0	ID=annotation1910576;Name=Fwd primer J31006;Parent=BBa_J31006
BBa_I715065	.	stop_codon	1	3	.	+	0	ID=annotation1910574;Name=stop;Parent=BBa_J31006
BBa_I715065	.	primer_binding_site	1	20	.	+	0	ID=annotation1910575;Name=Rev primer J31006;Parent=BBa_J31006
BBa_I715065	.	ribosome_entry_site	1499	1510	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I715065	.	conserved_region	1503	1506	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I715065	.	sequence_feature	1465	1490	.	+	0	ID=BBa_J44000;Name=BBa_J44000
BBa_I715065	.	CDS	1517	2236	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_I715065	.	CDS	1517	2236	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_I715065
ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatc
catgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggc
tggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgc
gggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaag
acgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtga
cgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcg
gtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcg
gcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctc
ccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgc
atgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagc
ccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtga
tgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctcc
aagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgc
atcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccg
gcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttaga
tttcattactagagtttctcctctttaattactagagttatcaaaaaccatggtttttgataatactagagcaatacgc
aaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtg
agcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtt
gtgtggaattgtgagcggataacaatttcacacatactagagttatcaaaaaccatggtttttgataatactagagaaa
gaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatg
ttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttg
cactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatac
ccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttca
aagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaagg
tattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatg
gcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcag
accattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgc
cctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggc
atggatgaactatacaaataataatactagagttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcg
ctttctagtgcaaattgtgaccgcattttg