BBa_I715065
type: ComponentDefinition
persistent identity: BBa I715065
display id: BBa_I715065
version: 1
was generated by: iGEM to SBOL conversion
description: Burnt Pancake Tester with RE and backwards Tet resistance
created: 2009-05-28T11:00:00Z
modified: 2015-08-31T04:07:50Z
mutable provenance: It comes from subparts in the registry.
owned by: james
owned by: myers
mutable description: This has backwards tet resistance (Tet_rev+RBS_rev) for testing backwards transcription from the promoter pLac. It also has a Hin-flippable promoter pLac.
discontinued: false
dominant: false
group u list: _120_
m user id: 0
owner id: 201
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: With the RE, recombination enhancer, the flipping goes faster. This is not the construct we used in our Burnt Pancake paper.
star: false
creator: Malcolm Campbell
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J44000
component: BBa_J44001
component: BBa_R0010
component: BBa_E0040
component: BBa_B0034
component: BBa_J3101
component: BBa_J31006
sequence annotation: BBa_J31006
sequence annotation: BBa_J44001
sequence annotation: BBa_J44000
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_J3101
sequence: https://synbiohub.org/public/igem/BBa_I715065_sequence/1

BBa_J44000
type: Component
persistent identity: component2003687
display id: component2003687
version: 1
top level: BBa_I715065
definition: hixC
access: public

BBa_J44001
type: Component
persistent identity: component2003686
display id: component2003686
version: 1
top level: BBa_I715065
definition: BBa_J44001
access: public

BBa_J44000
type: Component
persistent identity: component2003695
display id: component2003695
version: 1
top level: BBa_I715065
definition: hixC
access: public

BBa_R0010
type: Component
persistent identity: component2003688
display id: component2003688
version: 1
top level: BBa_I715065
definition: LacI
access: public

BBa_E0040
type: Component
persistent identity: component2003699
display id: component2003699
version: 1
top level: BBa_I715065
definition: GFP
access: public

BBa_B0034
type: Component
persistent identity: component2003697
display id: component2003697
version: 1
top level: BBa_I715065
definition: BBa_B0034
access: public

BBa_J3101
type: Component
persistent identity: component2003706
display id: component2003706
version: 1
top level: BBa_I715065
definition: RE
access: public

BBa_J31006
type: Component
persistent identity: component2003684
display id: component2003684
version: 1
top level: BBa_I715065
definition: tetA(C)b
access: public

BBa_J31006
type: SequenceAnnotation
persistent identity: annotation2003684
display id: annotation2003684
version: 1
top level: BBa_I715065
location: https://synbiohub.org/public/igem/BBa_I715065/annotation2003684/range2003684/1
component: BBa_J31006

https://synbiohub.org/public/igem/BBa_I715065/annotation2003684/range2003684/1
type: Range
persistent identity: range2003684
display id: range2003684
version: 1
top level: BBa_I715065
direction: direction/reverse
start: 1
end: 1191
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2003686
display id: annotation2003686
version: 1
top level: BBa_I715065
location: https://synbiohub.org/public/igem/BBa_I715065/annotation2003686/range2003686/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_I715065/annotation2003686/range2003686/1
type: Range
persistent identity: range2003686
display id: range2003686
version: 1
top level: BBa_I715065
direction: direction/reverse
start: 1200
end: 1214
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation2003687
display id: annotation2003687
version: 1
top level: BBa_I715065
location: https://synbiohub.org/public/igem/BBa_I715065/annotation2003687/range2003687/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715065/annotation2003687/range2003687/1
type: Range
persistent identity: range2003687
display id: range2003687
version: 1
top level: BBa_I715065
direction: direction/reverse
start: 1223
end: 1248
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2003688
display id: annotation2003688
version: 1
top level: BBa_I715065
location: https://synbiohub.org/public/igem/BBa_I715065/annotation2003688/range2003688/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I715065/annotation2003688/range2003688/1
type: Range
persistent identity: range2003688
display id: range2003688
version: 1
top level: BBa_I715065
direction: direction/forward
start: 1257
end: 1456
orientation: inline

BBa_J44000
type: SequenceAnnotation
persistent identity: annotation2003695
display id: annotation2003695
version: 1
top level: BBa_I715065
location: https://synbiohub.org/public/igem/BBa_I715065/annotation2003695/range2003695/1
component: BBa_J44000

https://synbiohub.org/public/igem/BBa_I715065/annotation2003695/range2003695/1
type: Range
persistent identity: range2003695
display id: range2003695
version: 1
top level: BBa_I715065
direction: direction/reverse
start: 1465
end: 1490
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2003697
display id: annotation2003697
version: 1
top level: BBa_I715065
location: https://synbiohub.org/public/igem/BBa_I715065/annotation2003697/range2003697/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I715065/annotation2003697/range2003697/1
type: Range
persistent identity: range2003697
display id: range2003697
version: 1
top level: BBa_I715065
direction: direction/reverse
start: 1499
end: 1510
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2003699
display id: annotation2003699
version: 1
top level: BBa_I715065
location: https://synbiohub.org/public/igem/BBa_I715065/annotation2003699/range2003699/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_I715065/annotation2003699/range2003699/1
type: Range
persistent identity: range2003699
display id: range2003699
version: 1
top level: BBa_I715065
direction: direction/reverse
start: 1517
end: 2236
orientation: inline

BBa_J3101
type: SequenceAnnotation
persistent identity: annotation2003706
display id: annotation2003706
version: 1
top level: BBa_I715065
location: https://synbiohub.org/public/igem/BBa_I715065/annotation2003706/range2003706/1
component: BBa_J3101

https://synbiohub.org/public/igem/BBa_I715065/annotation2003706/range2003706/1
type: Range
persistent identity: range2003706
display id: range2003706
version: 1
top level: BBa_I715065
direction: direction/reverse
start: 2245
end: 2321
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

hixC
type: ComponentDefinition
persistent identity: BBa J44000
display id: BBa_J44000
version: 1
was generated by: iGEM to SBOL conversion
description: hixC binding site for Salmonella typhimurium Hin recombinase
created: 2006-06-05T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [http://www.genetics.org/cgi/content/abstract/149/4/1649]
owned by: james
owned by: myers
mutable description: A 26 bp sequence of DNA composed of 12 bp inverted repeats and a 2 bp core that operates in Salmonella paired with a hixR binding site to recombine DNA. A second hix site is required for recombination to occur. The two sites bind Hin recombinase in the formation of an invertasome.
discontinued: false
dominant: true
experience: experience/Works
group u list: _71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Standard BioBrick prefix and suffix were added to the 26 bp sequence.
star: true
creator: Missouri Western and Davidson Groups, Todd Eckdahl
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_J44000_sequence/1

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse version of RBS BBa_B0030
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Repeats in oligos caused unusual products during cloning
star: true
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
top level: BBa_J44001
direction: direction/reverse
start: 1
end: 15
orientation: inline

tetA(C)b
type: ComponentDefinition
persistent identity: BBa J31006
display id: BBa_J31006
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
created: 2006-07-11T11:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: PsB1AT3
owned by: james
owned by: myers
mutable description: This is the coding region for Tetracyline resistance in the reverse orientation. If a promoter adn RBS are placed downstream and the entire region, from promoter to stop codon is flipped the cell will express tetracycline resistance.
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 919
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It was cloned into PsB1A2 plasmids.
star: true
creator: Sabriya Rosemond, Erin Zwack
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: stop
sequence annotation: tetA(C) backwards
sequence annotation: Rev primer J31006
sequence annotation: Fwd primer J31006
sequence: https://synbiohub.org/public/igem/BBa_J31006_sequence/1

stop
type: SequenceAnnotation
persistent identity: annotation1910574
display id: annotation1910574
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J31006/annotation1910574/range1910574/1
type: Range
persistent identity: range1910574
display id: range1910574
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 3
orientation: inline

tetA(C) backwards
type: SequenceAnnotation
persistent identity: annotation1884998
display id: annotation1884998
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31006/annotation1884998/range1884998/1
type: Range
persistent identity: range1884998
display id: range1884998
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1
end: 1191
orientation: inline

Rev primer J31006
type: SequenceAnnotation
persistent identity: annotation1910575
display id: annotation1910575
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31006/annotation1910575/range1910575/1
type: Range
persistent identity: range1910575
display id: range1910575
version: 1
top level: tetA(C)b
direction: direction/forward
start: 1
end: 20
orientation: inline

Fwd primer J31006
type: SequenceAnnotation
persistent identity: annotation1910576
display id: annotation1910576
version: 1
top level: tetA(C)b
location: https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31006/annotation1910576/range1910576/1
type: Range
persistent identity: range1910576
display id: range1910576
version: 1
top level: tetA(C)b
direction: direction/reverse
start: 1168
end: 1191
orientation: inline

RE
type: ComponentDefinition
persistent identity: BBa J3101
display id: BBa_J3101
version: 1
was generated by: iGEM to SBOL conversion
description: Recombinational Enhancer (RE) for Hin/Hix inverting
created: 2006-06-01T11:00:00Z
modified: 2015-08-31T04:08:45Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 918
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Erin Zwack, Sabriya Rosemond
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: RE Sequence
sequence annotation: Proximal Fis Binding Site
sequence annotation: Originally a C
sequence annotation: Former SpeI site
sequence annotation: Mutation of SpeI site
sequence annotation: Distal Fis Binding Site
sequence annotation: Insertion right before biobrick ends
sequence: https://synbiohub.org/public/igem/BBa_J3101_sequence/1

RE Sequence
type: SequenceAnnotation
persistent identity: annotation1884988
display id: annotation1884988
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884988/range1884988/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J3101/annotation1884988/range1884988/1
type: Range
persistent identity: range1884988
display id: range1884988
version: 1
top level: RE
direction: direction/forward
start: 1
end: 77
orientation: inline

Proximal Fis Binding Site
type: SequenceAnnotation
persistent identity: annotation1884991
display id: annotation1884991
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884991/range1884991/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J3101/annotation1884991/range1884991/1
type: Range
persistent identity: range1884991
display id: range1884991
version: 1
top level: RE
direction: direction/forward
start: 6
end: 21
orientation: inline

Originally a C
type: SequenceAnnotation
persistent identity: annotation1884989
display id: annotation1884989
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884989/range1884989/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J3101/annotation1884989/range1884989/1
type: Range
persistent identity: range1884989
display id: range1884989
version: 1
top level: RE
direction: direction/forward
start: 29
end: 29
orientation: inline

Former SpeI site
type: SequenceAnnotation
persistent identity: annotation1880471
display id: annotation1880471
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1880471/range1880471/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J3101/annotation1880471/range1880471/1
type: Range
persistent identity: range1880471
display id: range1880471
version: 1
top level: RE
direction: direction/forward
start: 51
end: 56
orientation: inline

Mutation of SpeI site
type: SequenceAnnotation
persistent identity: annotation1880472
display id: annotation1880472
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1880472/range1880472/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J3101/annotation1880472/range1880472/1
type: Range
persistent identity: range1880472
display id: range1880472
version: 1
top level: RE
direction: direction/forward
start: 51
end: 51
orientation: inline

Distal Fis Binding Site
type: SequenceAnnotation
persistent identity: annotation1884992
display id: annotation1884992
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884992/range1884992/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_J3101/annotation1884992/range1884992/1
type: Range
persistent identity: range1884992
display id: range1884992
version: 1
top level: RE
direction: direction/forward
start: 55
end: 69
orientation: inline

Insertion right before biobrick ends
type: SequenceAnnotation
persistent identity: annotation1884990
display id: annotation1884990
version: 1
top level: RE
location: https://synbiohub.org/public/igem/BBa_J3101/annotation1884990/range1884990/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J3101/annotation1884990/range1884990/1
type: Range
persistent identity: range1884990
display id: range1884990
version: 1
top level: RE
direction: direction/forward
start: 77
end: 77
orientation: inline

https://synbiohub.org/public/igem/BBa_I715065_sequence/1
type: Sequence
persistent identity: BBa I715065 sequence
display id: BBa_I715065_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcattactagagtttctcctctttaattactagagttatcaaaaaccatggtttttgataatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagttatcaaaaaccatggtttttgataatactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44000_sequence/1
type: Sequence
persistent identity: BBa J44000 sequence
display id: BBa_J44000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatcaaaaaccatggtttttgataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44001_sequence/1
type: Sequence
persistent identity: BBa J44001 sequence
display id: BBa_J44001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31006_sequence/1
type: Sequence
persistent identity: BBa J31006 sequence
display id: BBa_J31006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtcgaggtggcccggctccatgcaccgcgacgcaacgcggggaggcagacaaggtatagggcggcgcctacaatccatgccaacccgttccatgtgctcgccgaggcggcataaatcgccgtgacgatcagcggtccaatgatcgaagttaggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcggggccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgacgctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggcgcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcatgagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagcaaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatccacaggacgggtgtggtcgccatgatcgcgtagtcgatagtggctccaagtagcgaagcgagcaggactgggcggcggccaaagcggtcggacagtgctccgagaacgggtgcgcatagaaattgcatcaacgcatatagcgctagcagcacgccatagtgactggcgatgctgtcggaatggacgatatcccgcaagaggcccggcagtaccggcataaccaagcctatgcctacagcatccagggtgacggtgccgaggatgacgatgagcgcattgttagatttcat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J3101_sequence/1
type: Sequence
persistent identity: BBa J3101 sequence
display id: BBa_J3101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcgggtgtcaacaattgaccaaaatattgatttacagcgtaatgcgctttctagtgcaaattgtgaccgcattttg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z