LOCUS BBa_I715066 823 bp DNA linear 05-Nov-2024 DEFINITION It's a testing part for RE's effect on GFP. ACCESSION BBa_I715066 VERSION BBa_I715066.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..738 /label=GFP protein CDS 19..738 /label=BBa_E0040 misc_feature 797..802 /label=Former SpeI site misc_feature 797..797 /label=Mutation of SpeI site misc_feature 747..823 /label=RE Sequence misc_feature 775..775 /label=Originally a C misc_feature 823..823 /label=Insertion right before biobrick ends misc_feature 752..767 /label=Proximal Fis Binding Site misc_feature 801..815 /label=Distal Fis Binding Site misc_feature 747..823 /label=BBa_J3101 ORIGIN 1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 721 gaactataca aataataata ctagagttcg ggtgtcaaca attgaccaaa atattgattt 781 acagcgtaat gcgctttcta gtgcaaattg tgaccgcatt ttg //