LOCUS       BBa_I715067             1487 bp    DNA     linear       22-Dec-2024
DEFINITION  It is a flippable Lac Promoter.
ACCESSION   BBa_I715067
VERSION     BBa_I715067.1  
FEATURES             Location/Qualifiers
     CDS             1..1191
                     /label=tetA(C) backwards
     misc_feature    1..3
                     /label=stop
     primer_bind     1..20
                     /label=Rev primer J31006
     primer_bind     1168..1191
                     /label=Fwd primer J31006
     CDS             1..1191
                     /label=BBa_J31006
     misc_feature    1204..1207
                     /label=conserved
     RBS             1200..1211
                     /label=BBa_B0034
     misc_feature    1220..1245
                     /label=BBa_J44000
     CDS             1254..1341
                     /label=end of LacI coding region (inactive)
     misc_feature    1254..1453
                     /label=BBa_R0010
     misc_feature    1342..1379
                     /label=CAP binding site
     promoter        1390..1395
                     /label=-35
     promoter        1414..1419
                     /label=-10
     misc_feature    1419..1453
                     /label=LacI binding site
     misc_feature    1426..1426
                     /label=start
     promoter        1254..1453
                     /label=BBa_R0010
     misc_feature    1462..1487
                     /label=BBa_J44000
ORIGIN
        1 ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
       61 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
      121 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
      181 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
      241 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
      301 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
      361 cttctcgccg aaacgtttgg tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa
      421 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
      481 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
      541 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
      601 gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca
      661 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
      721 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
      781 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
      841 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
      901 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
      961 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     1021 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     1081 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     1141 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca ttactagaga
     1201 aagaggagaa atactagagt tatcaaaaac catggttttt gataatacta gagcaatacg
     1261 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     1321 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     1381 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     1441 acaatttcac acatactaga gttatcaaaa accatggttt ttgataa
//