LOCUS       BBa_I715070             2076 bp    DNA     linear       25-Nov-2024
DEFINITION  Flippable lac promoter with GFP
ACCESSION   BBa_I715070
VERSION     BBa_I715070.1  
FEATURES             Location/Qualifiers
     CDS             1..1191
                     /label=tetA(C) backwards
     misc_feature    1..3
                     /label=stop
     primer_bind     1..20
                     /label=Rev primer J31006
     primer_bind     1168..1191
                     /label=Fwd primer J31006
     CDS             1..1191
                     /label=BBa_J31006
     misc_feature    1204..1207
                     /label=conserved
     RBS             1200..1211
                     /label=BBa_B0034
     misc_feature    1220..1245
                     /label=BBa_J44000
     misc_feature    1258..1261
                     /label=conserved
     RBS             1254..1265
                     /label=BBa_B0034
     CDS             1272..1991
                     /label=GFP protein
     CDS             1272..1991
                     /label=BBa_E0040
     misc_feature    2050..2055
                     /label=Former SpeI site
     misc_feature    2050..2050
                     /label=Mutation of SpeI site
     misc_feature    2000..2076
                     /label=RE Sequence
     misc_feature    2028..2028
                     /label=Originally a C
     misc_feature    2076..2076
                     /label=Insertion right before biobrick ends
     misc_feature    2005..2020
                     /label=Proximal Fis Binding Site
     misc_feature    2054..2068
                     /label=Distal Fis Binding Site
     misc_feature    2000..2076
                     /label=BBa_J3101
ORIGIN
        1 ttaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta
       61 tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat
      121 cgccgtgacg atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc
      181 ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg
      241 catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt
      301 cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg
      361 cttctcgccg aaacgtttgg tggcggggcc agtgacgaag gcttgagcga gggcgtgcaa
      421 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
      481 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac
      541 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
      601 gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca
      661 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
      721 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
      781 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
      841 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
      901 ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc
      961 gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag
     1021 aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga
     1081 atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc
     1141 atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca ttactagaga
     1201 aagaggagaa atactagagt tatcaaaaac catggttttt gataatacta gagaaagagg
     1261 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1321 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     1381 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1441 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
     1501 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1561 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1621 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1681 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     1741 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     1801 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     1861 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     1921 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1981 acaaataata atactagagt tcgggtgtca acaattgacc aaaatattga tttacagcgt
     2041 aatgcgcttt ctagtgcaaa ttgtgaccgc attttg
//