BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961227 1 start range1961227 1 173 173 BBa_I715073 1 Ec83 msr/m Ec83 msr/msd region 2009-12-18T12:00:00Z 2015-08-31T04:07:50Z E. coli strain 161 Region of Ec83 retron that codes for a ssDNA/RNA hybrid after reverse transcription, the transcribed sequences acts as its own primer and template false false _120_ 0 1761 9 Not in stock false Part was synthesized by annealing oligonucleotides false Michael Waters BBa_I715075 1 BBa_I715075 pLac promoter upstream of the Ec83 retron msr/msd sequence 2010-05-07T11:00:00Z 2015-08-31T04:07:50Z The Ec86 retron comes from E.coli clinical strain B This part places the Ec86 msr/msd under the transcriptional control of the lac promoter. Transcription of the Ec86 msr/msd sequence results an mRNA strand that forms a secondary structure which allows it to act as a template and primer for reverse transcription. false false _120_ 0 1761 9 Not in stock false The Ec86 msr/msd BioBrick was designed using PCR false Michael Waters component2069007 1 BBa_R0010 component2069014 1 BBa_I715073 annotation2069007 1 BBa_R0010 range2069007 1 1 200 annotation2069014 1 BBa_I715073 range2069014 1 209 365 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_I715075_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagaggctctttagcgttttatggatttaccacctgattggtcaaatctagttgggcgttgcgcaaactctaatttattgattacatttacagttgcggaacaaactttttgagccgcaatccagtaggttgcggatcaaaaagtttgttccgcggcttcaa BBa_I715073_sequence 1 gctctttagcgttttatggatttaccacctgattggtcaaatctagttgggcgttgcgcaaactctaatttattgattacatttacagttgcggaacaaactttttgagccgcaatccagtaggttgcggatcaaaaagtttgttccgcggcttcaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z