BBa_I716016 1 BBa_I716016 (yfbE-rbs-RFP) jp paper style 2007-07-23T11:00:00Z 2015-08-31T04:07:50Z PCR product RFP_no_ATG + I716013 iron promoter yfbE with RBS and ATG on it, with an RFP without ATG added to the end false false _110_ 0 1812 9 Not in stock false <pre> make (yfbE-rbs-ATG) + (RFP_no_ATG) from pcr prod PCR product (RFP_no_ATG), digested BglII/XhoI Digest I716013 (yfbE-rbs-ATF) BamHI/XhoI (2387+9), L Ligate, get I716016 (yfbE-rbs-RFP_jp) or for rfp step, (I716015) 2063+693 get small. </pre> {{BerkiGEM2007-Biobrick2.0Intro}} false Arthur Yu BBa_I716016_sequence 1 gctaacacgtaaactccacctatagacaagcgcaaccagacaattaccgtgaaattgagctacatttctggcgataattcgcagttggtgtaatattaaaaatcctacgatgtcggcaaaatgcctcaaaattttgccaaatgcaaagcctaaataagaaaaaatataaaaatttcaatatttacgtctaatattagtttcttaaggttaagttaatattctatccttaaaatttcgctccaaatggcaaaatatacacaacactctttatagcaaatataagtggacaggtattcaatggcggaaggaaaagcaatgggatctgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z