BBa_I716071 1 BBa_I716071 pTrc-(NO SCAR)-rbs lib-beta-rbs lib-alpha 2007-06-26T11:00:00Z 2015-08-31T04:07:51Z Beta portion pcr CA1154F/G00101 on 9145-I716054 (575 bp) Product is ADtemp1 Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI) Product is ADtemp2 pcr CA1099F/D58 on ADtemp2 (3465 bp) Product is ADtemp3 _______________________________________________________ Alpha portion Pcr CA1150F/CA1099R on I716070 (1727 bp) Product is ADtemp5 ____________________________________________________________ Digest ADtemp3 (BsaI/BamHI) Digest ADtemp5 (BsaI/BglII) Ligate, product is: I716071 pTrc-(NO SCAR)-rbs lib-beta-rbs lib-alpha false false _110_ 0 1286 95 No part sequence false {{BerkiGEM2007-Biobrick2.0Intro}} false Austin Day igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z