LOCUS       BBa_I716101             3165 bp    DNA     linear       25-Dec-2024
DEFINITION  Low Copy Ampicillin Resistance Plasmid
ACCESSION   BBa_I716101
VERSION     BBa_I716101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..15
                     /label=Berkeley suffix
     misc_feature    3151..3165
                     /label=Berkeley prefix
     CDS             2053..2913
                     /label=beta-lactamase ampicillin resistance gene
     promoter        2955..2983
                     /label=beta-lactamase promoter
     CDS             217..1167
                     /label=repA
     misc_feature    1549..1916
                     /label=par region
     misc_feature    1901..1914
                     /label=inverted repeat
     misc_feature    1872..1888
                     /label=inverted repeat
     misc_feature    1838..1861
                     /label=inverted repeat
     misc_feature    1815..1832
                     /label=inverted repeat
     misc_feature    1336..1418
                     /label=AT rich region
     misc_feature    1318..1337
                     /label=direct repeat
     misc_feature    1297..1316
                     /label=direct repeat
     misc_feature    1265..1284
                     /label=direct repeat
     misc_feature    1194..1215
                     /label=inverted repeat
     promoter        1220..1225
                     /label=-35
     promoter        1198..1203
                     /label=-10
     promoter        1188..1207
                     /label=promoter region
     RBS             1171..1183
                     /label=RBS
     RBS             1169..1174
                     /label=RBS
     polyA_site      91..106
                     /label=putative polyA
     promoter        2957..2962
                     /label=-35
     promoter        2931..2936
                     /label=-10
     RBS             2918..2922
                     /label=RBS
     primer_bind     3033..3052
                     /label=VF2 primer binding site
ORIGIN
        1 ggatcctaac tcgaggttac attgtcgatc tgttcatggt gaacagcttt aaatgcacca
       61 aaaactcgta aaagctctga tgtatctatc ttttttacac cgttttcatc tgtgcatatg
      121 gacagttttc cctttgatat ctaacggtga acagttgttc tacttttgtt tgttagtctt
      181 gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt atttagccag
      241 tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc
      301 atgcttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc
      361 agttaaagca tcgtgtagtg tttttcttag tccgttacgt aggtaggaat ctgatgtaat
      421 ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag
      481 atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt
      541 atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac
      601 ccattggtta agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa
      661 ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa
      721 taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt
      781 atattttatg aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa
      841 ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt
      901 aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa
      961 tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa
     1021 cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca
     1081 gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt
     1141 tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact
     1201 ataccaattg agatgggcta gtcaatgata attacatgtc cttttccttt gagttgtggg
     1261 tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt
     1321 aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag
     1381 aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact
     1441 actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca
     1501 aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct
     1561 gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc
     1621 agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt
     1681 tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag
     1741 ggcgttttat ggcgggtctg ctatgtggtg ctatctgact ttttgctgtt cagcagttcc
     1801 tgccctctga ttttccagtc tgaccacttc ggattatccc gtgacaggtc attcagactg
     1861 gctaatgcac ccagtaaggc agcggtatca tcaacaggct tacccgtctt actgtcccta
     1921 gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     1981 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgtaaac
     2041 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2101 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2161 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2221 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2281 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2341 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2401 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2461 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2521 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2581 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2641 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2701 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2761 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2821 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2881 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2941 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     3001 acaaataggg gttccgcgca catttcccat ggtgccacct gacgtctaag aaaccattat
     3061 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
     3121 atccttagct ttcgctaagg atgatttctg gaattcatga gatct
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