LOCUS BBa_I716115 2980 bp DNA linear 26-Nov-2024 DEFINITION Iron controlled T7 RNA Polymerase ACCESSION BBa_I716115 VERSION BBa_I716115.1 FEATURES Location/Qualifiers promoter 20..283 /label=Iron Promoter (yfbE) misc_feature 329..2980 /label=T7rnap GTG Start RBS 309..322 /label=1106H RBS ORIGIN 1 gctaacacgt aaactccacc tatagacaag cgcaaccaga caattaccgt gaaattgagc 61 tacatttctg gcgataattc gcagttggtg taatattaaa aatcctacga tgtcggcaaa 121 atgcctcaaa attttgccaa atgcaaagcc taaataagaa aaaatataaa aatttcaata 181 tttacgtcta atattagttt cttaaggtta agttaatatt ctatccttaa aatttcgctc 241 caaatggcaa aatatacaca acactcttta tagcaaatat aagtggacag gtattcaatg 301 gcggatctgg ctaacatcgg tgggatctgt gaacacgatt aacatcgcta agaacgactt 361 ctctgacatc gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg 421 tttagctcgc gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt 481 ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa 541 gcctctcatc actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga 601 agtgaaagct aagcgcggca agcgcccgac agccttccag ttcctgcaag aaatcaagcc 661 ggaagccgta gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa 721 tacaaccgtt caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt 781 cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa 841 caagcgcgta gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct 901 ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca 961 tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg 1021 ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc 1081 tgaggctatc gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg 1141 cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg 1201 tcgtcctctg gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt 1261 ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa 1321 caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga 1381 catccctgcg attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc 1441 tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg 1501 caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca 1561 taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat 1621 gttcaacccg caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc 1681 aatcggtaag gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga 1741 taaggttccg ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc 1801 ttgcgctaag tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt 1861 ccttgcgttc tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc 1921 ccttccgctg gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg 1981 agatgaggta ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta 2041 cgggattgtt gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga 2101 taacgaagta gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct 2161 gggcactaag gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa 2221 gcgttcagtc atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct 2281 ggaagatacc attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa 2341 tcaggctgct ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc 2401 tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa 2461 agataagaag actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga 2521 tggtttccct gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt 2581 cctcggtcag ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc 2641 acacaaacag gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct 2701 tcgtaagact gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca 2761 cgactccttc ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac 2821 tatggttgac acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga 2881 ccagttgcac gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa 2941 cctccgtgac atcttagagt cggacttcgc gttcgcgtaa //