BBa_I716253 1 BBa_I716253 HPI, katG 2007-06-05T11:00:00Z 2015-08-31T04:07:51Z Salmonella typhimurium LT2 A basic part required for the chaperone/toxicity device false false _110_ 0 1696 9 It's complicated false {{BerkiGEM2007-Biobrick2.0Intro}} Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the HPI, katG open reading frame from Salmonella typhimurium LT2 PCR VU03F.katG /VU06R.katG on HPI, katG (1316 bp, gp = A) PCR VU05F.katG/VU04R.katG on HPI, katG (924 bp, gp = B) --------------------------------------------------- PCR VU03F/VU04R on A+B (2212 bp, BglII/XhoI) Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L) Product is pBca9145-VU2 --------------------------------------------------- VU03F.katG (External oligo) ccaaaAGATCTatgagcacgaccgacgataccc VU04R.katG (External oligo) gctagCTCGAGttaGGATCCttattgcagatcgaaacggtcc VU05F.katG (Internal oligo) gccgaaagaagaCctgatctggcag VU06R.katG (Internal oligo) ctgccagatcagGtcttctttcggc false Vaibhavi Umesh BBa_I716253_sequence 1 atgagcacgaccgacgatacccataacacgttatccactggaaaatgtcctttccatcagggggggcatgaccgaagcgcaggcgcagggactgccagccgcgactggtggccgaaccagcttcgcgtggatcttttgaatcaacattccaaccgttctaacccgctgggtgaagactttgactaccgcaaagagtttagcaagttagactactccgccctgaaaggggatctcaaggcgctgctgaccgattcacaaccgtggtggcccgctgactggggcagctatgtcggtttgtttattcgcatggcctggcatggcgctggcacctaccgttctattgatggtcgtggcggcgcgggtcgtggtcaacagcgttttgcgccgcttaactcctggccggataacgtcagcctggataaggcgcgtcgtttgttgtggccgattaagcagaaatatggccagaaaatttcctgggccgacctgtttattctggcgggtaacgtggcgctggaaaactccggcttccgtaccttcggtttcggcgccgggcgtgaagatgtctgggaaccggatctggatgtgaactggggcgatgaaaaagcctggttgactcaccgacaccctgaagcgctggcaaaagcgccgctgggcgcgaccgagatgggccttatctacgttaacccggaagggccggatcacagcggcgaaccactttctgccgccgccgctattcgcgctacctttggcaatatggggatgaacgacgaagagaccgtggcgttgatcgctggcgggcataccctcggtaaaacccacggcgcggcagcggcatcccatgtaggggccgatccggaagccgcgccgattgaagcgcagggcttaggttgggccagcagctatggtagtggcgttggcgcggatgctatcacctccgggctggaagtggtctggacgcagacgccgacccagtggagcaactatttcttcgagaacctgttcaaatatgagtgggtacaaacccgtagtccggctggcgctatccagtttgaagcggtagacgcgccggacatcatcccggacccgttcgatccgtcgaaaaaacgtaagccaaccatgctggtcaccgacctgacgctgcgttttgatccggagttcgagaagatttcccgtcgtttccttaacgatccgcaggcctttaatgaagcctttgctcgtgcctggttcaaactgacgcacagagatatgggaccaaaagcgcgttacatcggaccggaagtgccgaaagaagatctgatctggcaggacccgttgccgcaaccgctctatcagccaacgcaggaagacattatcaacctgaaagcggcgatcgctgcatccgggctttctattagcgagatggtttcggttgcctgggcatccgcgtctactttccgcggcggcgataagcgtggcggcgctaacggcgcgcgtctggcattagcgcctcagcgcgactgggatgtcaacgccgttgcggctcgcgttctgccggtattagaagagatccagaaaacgacgaataaagcctcgctggccgatattattgtgctggcgggcgtggtcggtatcgagcaggcggccgctgctgcgggtgtcagcatcagcgtaccttttgcgccgggccgggtggatgcgcgtcaggatcagaccgacatcgagatgttctcgctgcttgaaccgattgctgatggattccgtaactatcgtgcgcgtctggatgtgtcgacgaccgaatcgctgttgattgataaagcgcagcagttaacgttgaccgcgccggaaatgacggtactggttggcgggatgcgtgtgctgggaaccaactttgacggcagccagaacggtgtctttaccgacagaccgggcgtgctcagcactgacttcttcgctaatctgctggatatgcgttacgagtggaagcccaccgacgacgctaatgagctgttcgaaggccgggatcgtctgactggcgaggtaaaatacacggcgacccgcgccgatctggtgtttggttccaactccgtactgcgcgcgctggcggaagtttacgcgtgtagcgatgcgcacgagaagtttgtgaaggacttcgtcgcggcatgggtgaaagtgatgaacctggaccgtttcgatctgcaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z