BBa_I716303 1 BBa_I716303 otsBA 2007-07-23T11:00:00Z 2015-08-31T04:07:52Z {{BerkiGEM2007-Biobrick2.0Intro}} The operon that codes for Trehalose as it is naturally found on the E.coli genome. false false _110_ 0 2093 9 It's complicated false PCR otsBF/otsBIR on otsBA (485 bp, gp = A) PCR otsBIF/otsAIR on otsBA (720 bp, gp = B) PCR otsAR/otsAIF on otsBA (413 bp, gp = C) ----------------------------------------------- PCR otsBF/otsAR on A+B+C (2200 bp, BglII/XhoI) Digest Pbca9145-Bca1144 (BglII/XhoI, 2054+910, L) Product is Pbca9145- BBa I716303 ----------------------------------------------- otsBF Forward BglII site anneals to otsB cgtaaAGATCTATGACAGAACCGTTAACCGA otsBIF Removes internal BglII site GCAACGTATTACTCAGATtTGGCCACAAATGGCG otsBIR Removes internal BglII site CGCCATTTGTGGCCAaATCTGAGTAATACGTTGC otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC false vincent parker BBa_I716303_sequence 1 gaattccgtaaagatctatgacagaaccgttaaccgaaacccctgaactatccgcgaaatatgcctggttttttgatcttgatggaacgctggcggaaatcaaaccgcatcccgatcaggtcgtcgtgcctgacaatattctgcaaggactacagctactggcaaccgcaagtgatggtgcattggcattgatatcagggcgctcaatggtggagcttgacgcactggcaaaaccttatcgcttcccgttagcgggcgtgcatggggcggagcgccgtgacatcaatggtaaaacacatatcgttcatctgccggatgcgattgcgcgtgatattagcgtgcaactgcatacagtcatcgctcagtatcccggcgcggagctggaggcgaaagggatggcttttgcgctgcattatcgtcgggctccgcagcatgaagacgcattaatgacattagcgcaacgtattactcagatttggccacaaatggcgttacagcagggaaagtgtgttgtcgagatcaaaccgagaggtaccagtaaaggtgaggcaattgcagcttttatgcaggaagctccctttatcgggcgaacgcccgtatttctgggcgatgatttaaccgatgaatctggcttcgcagtcgttaaccgactgggcggaatgtcagtaaaaattggcacaggtgcaactcaggcatcatggcgactggcgggtgtgccggatgtctggagctggcttgaaatgataaccaccgcattacaacaaaaaagagaaaataacaggagtgatgactatgagtcgtttagtcgtagtatctaaccggattgcaccaccagacgagcacgccgccagtgccggtggccttgccgttggcatactgggggcactgaaagccgcaggcggactgtggtttggctggagtggtgaaacagggaatgaggatcagccgctaaaaaaggtgaaaaaaggtaacattacgtgggcctcttttaacctcagcgaacaggaccttgacgaatactacaaccaattctccaatgccgttctctggcccgcttttcattatcggctcgatctggtgcaatttcagcgtcctgcctgggacggctatctacgcgtaaatgcgttgctggcagataaattactgccgctgttgcaagacgatgacattatctggattcacgattatcacctgttgccatttgcgcatgaattacgcaaacggggagtgaataatcgcattggtttctttctgcatattcctttcccgacaccggaaatcttcaacgcgctgccgacatatgacaccttgcttgaacagctttgtgattatgatttgctgggtttccagacagaaaacgatcgtctggcgttcctggattgtctttctaacctgacccgcgtcacgacacgtagcgcaaaaagccatacagcctggggcaaagcatttcgaacagaagtctacccgatcggcattgaaccgaaagaaatagccaaacaggctgccgggccactgccgccaaaactggcgcaacttaaagcggaactgaaaaacgtacaaaatatcttttctgtcgaacggctggattattccaaaggtttgccagagcgttttctcgcctatgaagcgttgctggaaaaatatccgcagcatcatggtaaaattcgttatacccagattgcaccaacgtcgcgtggtgatgtgcaagcctatcaggatattcgtcatcagctcgaaaatgaagctggacgaattaatggtaaatacgggcaattaggctggacgccgctttattatttgaatcagcattttgaccgtaaattactgatgaaaatattccgctactctgacgtgggcttagtgacgccactgcgtgacgggatgaacctggtagcaaaagagtatgttgctgctcaggacccagccaatccgggcgttcttgttctttcgcaatttgcgggagcggcaaacgagttaacgtcggcgttaattgttaacccctacgatcgtgacgaagttgcagctgcgctggatcgtgcattgactatgtcgctggcggaacgtatttcccgtcatgcagaaatgctggacgttatcgtgaaaaacgatattaaccactggcaggagtgcttcattagcgacctaaagcagatagttccgcgaagcgcggaaagccagcagcgcgataaagttgctacctttccaaagcttgcgtaaggatcctaactcgagatacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z